##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccI AclI AgeI AhdI
AscI AseI AsnI AspEI AspI
AviII AvrII BalI BclI BglII
BlnI BsaBI BsiWI Bsp1407I BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
FbaI FseI FspI MamI MfeI
MluI MluNI MscI MunI NcoI
NdeI NotI NruI NspI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI SalI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
Acc65I 3730 GGTACC G^GTACC
AccIII 775 TCCGGA T^CCGGA
AcyI 1970 GGCGCC G[GA]^CG[CT]C
AflII 1677 CTTAAG C^TTAAG
AflIII 3579 ACACGT A^C[GA][CT]GT
Alw44I 489 GTGCAC G^TGCAC
AocI 2013 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2631 AGCGCT AGC^GCT
ApaI 66 GGGCCC GGGCC^C
ApaLI 489 GTGCAC G^TGCAC
Asp718I 3730 GGTACC G^GTACC
BamHI 2819 GGATCC G^GATCC
BbeI 1970 GGCGCC GGCGC^C
BbrPI 3580 CACGTG CAC^GTG
BcgI 94 CGAGGCACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2672 GTATCC GTATCC(6/5)
BfrI 1677 CTTAAG C^TTAAG
BsaAI 3580 CACGTG [CT]AC^GT[GA]
BsaHI 1970 GGCGCC G[GA]^CG[CT]C
BseAI 775 TCCGGA T^CCGGA
BsgI 1923 GTGCAG GTGCAG(16/14)
BsiEI 3097 CGGTCG CG[GA][CT]^CG
BspDI 225 ATCGAT AT^CGAT
BspEI 775 TCCGGA T^CCGGA
BsrBI 1471 CCGCTC CCGCTC(-3/-3)
BsrDI 1151 CATTGC GCAATG(2/0)
BssSI 3691 CTCGTG CACGAG(-5/-1)
BstYI 2819 GGATCC [GA]^GATC[CT]
Bsu36I 2013 CCTCAGG CC^T[ACGT]AGG
ClaI 225 ATCGAT AT^CGAT
CpoI 115 CGGTCCG CG^G[AT]CCG
DraIII 3997 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2631 AGCGCT AGC^GCT
Eco81I 2013 CCTCAGG CC^T[ACGT]AGG
EcoRI 1903 GAATTC G^AATTC
EcoT22I 1203 ATGCAT ATGCA^T
Hin1I 1970 GGCGCC G[GA]^CG[CT]C
HincII 4096 GTTAAC GT[CT]^[GA]AC
HindII 4096 GTTAAC GT[CT]^[GA]AC
HindIII 2933 AAGCTT A^AGCTT
HpaI 4096 GTTAAC GTT^AAC
KasI 1970 GGCGCC G^GCGCC
KpnI 3730 GGTACC GGTAC^C
KspI 2187 CCGCGG CCGC^GG
MflI 2819 GGATCC [GA]^GATC[CT]
MroI 775 TCCGGA T^CCGGA
NaeI 1973 GCCGGC GCC^GGC
NarI 1970 GGCGCC GG^CGCC
NgoMIV 1973 GCCGGC G^CCGGC
NheI 2001 GCTAGC G^CTAGC
NsiI 1203 ATGCAT ATGCA^T
PaeR7I 3116 CTCGAG C^TCGAG
PmaCI 3580 CACGTG CAC^GTG
PmlI 3580 CACGTG CAC^GTG
PshAI 3159 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 115 CGGTCCG CG^G[AT]CCG
SacII 2187 CCGCGG CCGC^GG
SapI 3675 GCTCTTC GCTCTTC(1/4)
SfoI 1970 GGCGCC GGC^GCC
SgrAI 1972 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2497 CCCGGG CCC^GGG
SspI 4069 AATATT AAT^ATT
XbaI 1907 TCTAGA T^CTAGA
XhoI 3116 CTCGAG C^TCGAG
XhoII 2819 GGATCC [GA]^GATC[CT]
XmaCI 2497 CCCGGG C^CCGGG
XmaI 2497 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Asp700I 1571 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3148 GAAGACATTC
BglI 1479 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1755 GCCATGCAGGC
BlpI 1155 GCTGAGC GC^T[ACGT]AGC
3277 GCTGAGC
Bpu1102I 1155 GCTGAGC GC^T[ACGT]AGC
3277 GCTGAGC
BsmBI 2045 CGTCTC CGTCTC(1/5)
1514 GAGACG
BstAPI 968 GCAGGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2201 GCAGCAGCTGC
BstXI 1540 CCAATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2975 CCACCCAACTGG
CelII 1155 GCTGAGC GC^T[ACGT]AGC
3277 GCTGAGC
EaeI 407 TGGCCG [CT]^GGCC[GA]
1127 TGGCCG
MaeII 1258 ACGT A^CGT
3581 ACGT
PflMI 1278 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3996 CCACCATGTGG
PpuMI 1645 GGGACCT [GA]G^G[AT]CC[CT]
1993 AGGACCT
StuI 1264 AGGCCT AGG^CCT
2266 AGGCCT
Van91I 1278 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3996 CCACCATGTGG
XmnI 1571 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3148 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI