##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI AvrII BamHI BbeI
BcgI BfrI BglII BlnI BsiWI
BspDI BspLU11I BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
EcoO65I EcoT22I FseI HindIII HpaI
KasI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
RsrII SalI SbfI ScaI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 3927 TCCGGA T^CCGGA
AcsI 2117 GAATTC [GA]^AATT[CT]
AflIII 4195 ACGCGT A^C[GA][CT]GT
ApoI 2117 GAATTC [GA]^AATT[CT]
AspI 2309 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4317 TGCGCA TGC^GCA
BciVI 1033 GGATAC GTATCC(6/5)
BsaBI 1034 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3927 TCCGGA T^CCGGA
BsmBI 1726 CGTCTC CGTCTC(1/5)
BsmI 2519 GCATTC GAATGC(1/-1)
BspEI 3927 TCCGGA T^CCGGA
BspHI 2491 TCATGA T^CATGA
BsrDI 510 CATTGC GCAATG(2/0)
BssHII 3011 GCGCGC G^CGCGC
EcoRI 2117 GAATTC G^AATTC
EcoRV 2882 GATATC GAT^ATC
FspI 4317 TGCGCA TGC^GCA
KspI 4296 CCGCGG CCGC^GG
MamI 1034 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3297 CAATTG C^AATTG
MluI 4195 ACGCGT A^CGCGT
MroI 3927 TCCGGA T^CCGGA
MunI 3297 CAATTG C^AATTG
NspI 3484 GCATGC [GA]CATG^[CT]
PflFI 2309 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 3877 CGATCG CGAT^CG
RcaI 2491 TCATGA T^CATGA
SacII 4296 CCGCGG CCGC^GG
SfiI 3757 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3484 GCATGC GCATG^C
Tth111I 2309 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1387 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 3174 GGTACC G^GTACC
4098 GGTACC
AccI 1573 GTCTAC GT^[AC][GT]AC
4007 GTCTAC
AhdI 2849 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4301 GACCCTCTGTC
Alw44I 3020 GTGCAC G^TGCAC
3284 GTGCAC
ApaLI 3020 GTGCAC G^TGCAC
3284 GTGCAC
Asp718I 3174 GGTACC G^GTACC
4098 GGTACC
AspEI 2849 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4301 GACCCTCTGTC
BbrPI 1112 CACGTG CAC^GTG
3044 CACGTG
BclI 1039 TGATCA T^GATCA
1146 TGATCA
BsaWI 2133 ACCGGA [AT]^CCGG[AT]
3927 TCCGGA
BsiEI 2202 CGACCG CG[GA][CT]^CG
3877 CGATCG
Bsp1407I 2436 TGTACA T^GTACA
2772 TGTACA
BsrGI 2436 TGTACA T^GTACA
2772 TGTACA
BstAPI 1210 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2101 GCAGACGCTGC
Eam1105I 2849 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4301 GACCCTCTGTC
FbaI 1039 TGATCA T^GATCA
1146 TGATCA
HincII 3206 GTCAAC GT[CT]^[GA]AC
3650 GTCAAC
HindII 3206 GTCAAC GT[CT]^[GA]AC
3650 GTCAAC
KpnI 3174 GGTACC GGTAC^C
4098 GGTACC
NaeI 2243 GCCGGC GCC^GGC
2286 GCCGGC
NgoMIV 2243 GCCGGC G^CCGGC
2286 GCCGGC
PmaCI 1112 CACGTG CAC^GTG
3044 CACGTG
PmlI 1112 CACGTG CAC^GTG
3044 CACGTG
PvuII 1796 CAGCTG CAG^CTG
3901 CAGCTG
SexAI 2736 ACCAGGT A^CC[AT]GGT
3735 ACCTGGT
SmlI 3026 CTCAAG C^T[CT][GA]AG
3068 CTCAAG
SspBI 2436 TGTACA T^GTACA
2772 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco47III
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI