##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AclI AgeI AhdI AocI Aor51HI
AscI AseI AsnI AspEI AviII
AvrII BciVI BclI BlnI BsaBI
BsiWI BsmI BspDI BspHI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRV FbaI
FspI HincII HindII HindIII HpaI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AatII 1048 GACGTC GACGT^C
Acc65I 1445 GGTACC G^GTACC
AflII 2881 CTTAAG C^TTAAG
Alw44I 1057 GTGCAC G^TGCAC
ApaI 2786 GGGCCC GGGCC^C
ApaLI 1057 GTGCAC G^TGCAC
Asp718I 1445 GGTACC G^GTACC
BamHI 2068 GGATCC G^GATCC
BfrI 2881 CTTAAG C^TTAAG
Bsp1407I 1187 TGTACA T^GTACA
BsrBI 1666 GAGCGG CCGCTC(-3/-3)
BsrGI 1187 TGTACA T^GTACA
BstAPI 111 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 774 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2205 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 312 ATGCAT ATGCA^T
FseI 540 GGCCGGCC GGCCGG^CC
KpnI 1445 GGTACC GGTAC^C
NcoI 881 CCATGG C^CATGG
NsiI 312 ATGCAT ATGCA^T
SexAI 775 ACCTGGT A^CC[AT]GGT
SfiI 531 GGCCGTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 1187 TGTACA T^GTACA
SspI 3721 AATATT AAT^ATT
StuI 1103 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 79 GTCTAC GT^[AC][GT]AC
3369 GTCTAC
AccIII 370 TCCGGA T^CCGGA
3569 TCCGGA
BbeI 91 GGCGCC GGCGC^C
862 GGCGCC
BbrPI 2119 CACGTG CAC^GTG
4357 CACGTG
BcgI 37 GCAGCCATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1016 GCAAGGACATCG
BglII 1757 AGATCT A^GATCT
1850 AGATCT
BseAI 370 TCCGGA T^CCGGA
3569 TCCGGA
BspEI 370 TCCGGA T^CCGGA
3569 TCCGGA
BssSI 3029 CTCGTG CACGAG(-5/-1)
3364 CTCGTG
BstXI 1454 CCATCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1616 CCACCTTCCTGG
DraI 3686 TTTAAA TTT^AAA
3716 TTTAAA
EcoRI 421 GAATTC G^AATTC
3012 GAATTC
KasI 91 GGCGCC G^GCGCC
862 GGCGCC
KspI 95 CCGCGG CCGC^GG
129 CCGCGG
MroI 370 TCCGGA T^CCGGA
3569 TCCGGA
NarI 91 GGCGCC GG^CGCC
862 GGCGCC
PmaCI 2119 CACGTG CAC^GTG
4357 CACGTG
PmlI 2119 CACGTG CAC^GTG
4357 CACGTG
PpuMI 150 AGGTCCC [GA]G^G[AT]CC[CT]
3041 GGGACCT
SacII 95 CCGCGG CCGC^GG
129 CCGCGG
ScaI 1160 AGTACT AGT^ACT
2021 AGTACT
SfoI 91 GGCGCC GGC^GCC
862 GGCGCC
SmaI 514 CCCGGG CCC^GGG
2782 CCCGGG
SphI 1232 GCATGC GCATG^C
1893 GCATGC
XmaCI 514 CCCGGG C^CCGGG
2782 CCCGGG
XmaI 514 CCCGGG C^CCGGG
2782 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmnI