##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AhdI Aor51HI AscI Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BcgI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BspDI
BsrBI BsrFI BssHII BssSI BstBI
BstEII BstPI BstUI CelII Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FauI FseI FspI Hin1I HpaI
KasI KpnI KspI MamI MluI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 2837 TCCGGA T^CCGGA
AflII 504 CTTAAG C^TTAAG
Alw44I 2490 GTGCAC G^TGCAC
ApaI 230 GGGCCC GGGCC^C
ApaLI 2490 GTGCAC G^TGCAC
AspI 2440 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2933 TGGCCA TGG^CCA
BbvCI 2673 CCTCAGC CCTCAGC(-5/-2)
BfrI 504 CTTAAG C^TTAAG
BseAI 2837 TCCGGA T^CCGGA
BsmI 1742 GCATTC GAATGC(1/-1)
Bsp1407I 3648 TGTACA T^GTACA
BspEI 2837 TCCGGA T^CCGGA
BspLU11I 3803 ACATGT A^CATGT
BspMI 1771 GCAGGT ACCTGC(4/8)
BsrGI 3648 TGTACA T^GTACA
BstYI 727 AGATCC [GA]^GATC[CT]
CfoI 1804 GCGC GCG^C
DrdI 2733 GACAGCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2933 TGGCCA [CT]^GGCC[GA]
EcoNI 2875 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1803 GGCGCT [GA]GCGC^[CT]
HgaI 3133 GACGC GACGC(5/10)
HhaI 1804 GCGC GCG^C
HinP1I 1804 GCGC G^CGC
MfeI 511 CAATTG C^AATTG
MflI 727 AGATCC [GA]^GATC[CT]
MluNI 2933 TGGCCA TGG^CCA
MroI 2837 TCCGGA T^CCGGA
MscI 2933 TGGCCA TGG^CCA
MunI 511 CAATTG C^AATTG
NcoI 2347 CCATGG C^CATGG
PflFI 2440 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2919 CCAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1812 GCTCTTC GCTCTTC(1/4)
SexAI 1836 ACCTGGT A^CC[AT]GGT
SspBI 3648 TGTACA T^GTACA
SwaI 3917 ATTTAAAT ATTT^AAAT
Tth111I 2440 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2919 CCAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4011 TCTAGA T^CTAGA
XhoII 727 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AocI 165 CCTAAGG CC^T[ACGT]AGG
225 CCTAAGG
Asp700I 1218 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4332 GAATTTTTTC
AvaI 2529 CTCGAG C^[CT]CG[GA]G
2655 CTCGAG
AvaII 1674 GGACC G^G[AT]CC
1840 GGTCC
BanI 2660 GGCACC G^G[CT][GA]CC
4103 GGTGCC
BciVI 2336 GGATAC GTATCC(6/5)
2351 GGATAC
BclI 1717 TGATCA T^GATCA
2473 TGATCA
BsaI 539 GAGACC GGTCTC(1/5)
668 GGTCTC
BsaWI 847 ACCGGA [AT]^CCGG[AT]
2837 TCCGGA
BseRI 1866 GAGGAG GAGGAG(10/8)
2882 GAGGAG
BsoBI 2529 CTCGAG C^[CT]CG[GA]G
2655 CTCGAG
BspHI 1214 TCATGA T^CATGA
3665 TCATGA
BstAPI 588 GCACATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1455 GCAGCTTTTGC
BstXI 316 CCAATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
886 CCATTAAACTGG
Bsu36I 165 CCTAAGG CC^T[ACGT]AGG
225 CCTAAGG
DraII 229 AGGGCCC [GA]G^G[ACGT]CC[CT]
230 GGGCCCC
DraIII 258 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1721 CACATTGTG
Eco81I 165 CCTAAGG CC^T[ACGT]AGG
225 CCTAAGG
EcoO109I 229 AGGGCCC [GA]G^G[ACGT]CC[CT]
230 GGGCCCC
EcoRI 572 GAATTC G^AATTC
3082 GAATTC
FbaI 1717 TGATCA T^GATCA
2473 TGATCA
HapII 848 CCGG C^CGG
2838 CCGG
HincII 516 GTTGAC GT[CT]^[GA]AC
1116 GTTGAC
HindII 516 GTTGAC GT[CT]^[GA]AC
1116 GTTGAC
HindIII 547 AAGCTT A^AGCTT
1627 AAGCTT
HpaII 848 CCGG C^CGG
2838 CCGG
MspI 848 CCGG C^CGG
2838 CCGG
NdeI 567 CATATG CA^TATG
3632 CATATG
PaeR7I 2529 CTCGAG C^TCGAG
2655 CTCGAG
PstI 417 CTGCAG CTGCA^G
3382 CTGCAG
RcaI 1214 TCATGA T^CATGA
3665 TCATGA
SacI 2532 GAGCTC GAGCT^C
2637 GAGCTC
XhoI 2529 CTCGAG C^TCGAG
2655 CTCGAG
XmnI 1218 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4332 GAATTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI AspHI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
Bst1107I BstF5I BstNI BstZ17I Cac8I
Cfr13I DdeI DpnII DraI DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PshBI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I