##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AflII AgeI Aor51HI AscI
AseI AsnI AviII BbvCI BclI
BfrI BglI BsiEI BsiWI BspDI
BssHII BstBI ClaI CpoI DraI
EagI EclXI Eco47III Eco52I EcoRI
FbaI FseI FspI HpaI KspI
MfeI MunI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI PvuI RsrII
SacII SalI SfiI SfuI SgrAI
SmaI SnaBI SpeI SspI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 6567 GGTACC G^GTACC
AccII 5652 CGCG CG^CG
AccIII 3065 TCCGGA T^CCGGA
AhdI 5017 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3716 GGGCCC GGGCC^C
Asp718I 6567 GGTACC G^GTACC
AspEI 5017 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 5112 CCTAGG C^CTAGG
BbeI 5100 GGCGCC GGCGC^C
BbrPI 53 CACGTG CAC^GTG
BciVI 4662 GGATAC GTATCC(6/5)
BlnI 5112 CCTAGG C^CTAGG
BsaBI 1156 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3065 TCCGGA [AT]^CCGG[AT]
BseAI 3065 TCCGGA T^CCGGA
BspEI 3065 TCCGGA T^CCGGA
BspLU11I 1889 ACATGT A^CATGT
BsrBI 4114 GAGCGG CCGCTC(-3/-3)
BsrFI 2936 GCCGGT [GA]^CCGG[CT]
BstEII 5514 GGTCACC G^GT[ACGT]ACC
BstPI 5514 GGTCACC G^GT[ACGT]ACC
BstUI 5652 CGCG CG^CG
Cfr10I 2936 GCCGGT [GA]^CCGG[CT]
DraIII 680 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 5017 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5514 GGTCACC G^GT[ACGT]ACC
EcoRV 58 GATATC GAT^ATC
KasI 5100 GGCGCC G^GCGCC
KpnI 6567 GGTACC GGTAC^C
MamI 1156 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 5651 ACGCGT A^CGCGT
MroI 3065 TCCGGA T^CCGGA
MvnI 5652 CGCG CG^CG
NarI 5100 GGCGCC GG^CGCC
NdeI 1733 CATATG CA^TATG
PmaCI 53 CACGTG CAC^GTG
PmlI 53 CACGTG CAC^GTG
PshAI 1537 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 5100 GGCGCC GGC^GCC
StuI 5441 AGGCCT AGG^CCT
XbaI 6640 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AclI 2848 AACGTT AA^CGTT
5585 AACGTT
AcyI 4556 GACGCC G[GA]^CG[CT]C
5100 GGCGCC
Alw44I 677 GTGCAC G^TGCAC
3232 GTGCAC
ApaLI 677 GTGCAC G^TGCAC
3232 GTGCAC
Asp700I 3105 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4800 GAAGGATTTC
AspI 6163 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6723 GACCCAGTC
AvaI 449 CTCGGG C^[CT]CG[GA]G
1438 CCCGAG
BsaAI 53 CACGTG [CT]AC^GT[GA]
1664 TACGTG
BsaHI 4556 GACGCC G[GA]^CG[CT]C
5100 GGCGCC
BsmBI 4361 CGTCTC CGTCTC(1/5)
1150 GAGACG
BsoBI 449 CTCGGG C^[CT]CG[GA]G
1438 CCCGAG
BssSI 2056 CTCGTG CACGAG(-5/-1)
6516 CTCGTG
DrdI 364 GACGAGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1610 GACTCATTGGTC
EcoT22I 461 ATGCAT ATGCA^T
4864 ATGCAT
Hin1I 4556 GACGCC G[GA]^CG[CT]C
5100 GGCGCC
HincII 1669 GTTGAC GT[CT]^[GA]AC
2044 GTTGAC
HindII 1669 GTTGAC GT[CT]^[GA]AC
2044 GTTGAC
HindIII 1556 AAGCTT A^AGCTT
2356 AAGCTT
NsiI 461 ATGCAT ATGCA^T
4864 ATGCAT
PflFI 6163 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6723 GACCCAGTC
Psp1406I 2848 AACGTT AA^CGTT
5585 AACGTT
SacI 3780 GAGCTC GAGCT^C
5158 GAGCTC
SbfI 4986 CCTGCAGG CCTGCA^GG
5352 CCTGCAGG
Sse8387I 4986 CCTGCAGG CCTGCA^GG
5352 CCTGCAGG
Tth111I 6163 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6723 GACCCAGTC
XmnI 3105 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4800 GAAGGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BalI BamHI BanI
BanII BbsI BbvI BcgI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrGI BsrI BssKI
Bst1107I BstAPI BstF5I BstNI BstXI
BstYI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SexAI SfaNI SfcI SmlI SphI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII