##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbvCI
BcgI BfrI BglII BlnI BseAI
BsiWI BspEI BspHI BspLU11I Bst1107I
BstBI BstEII BstPI BstZ17I DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FseI FspI HindIII HpaI
KpnI MluI MroI NotI NruI
NspV PacI PflFI PinAI PmeI
PshAI PshBI PvuI RcaI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 3636 GACGTC GACGT^C
AccI 2880 GTCGAC GT^[AC][GT]AC
AclI 920 AACGTT AA^CGTT
AocI 3948 CCTGAGG CC^T[ACGT]AGG
Asp700I 1743 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 1092 CACGTG CAC^GTG
BciVI 367 GTATCC GTATCC(6/5)
BclI 817 TGATCA T^GATCA
BsaBI 362 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 302 ACCGGA [AT]^CCGG[AT]
BsiEI 2833 CGACCG CG[GA][CT]^CG
BspMI 1431 ACCTGC ACCTGC(4/8)
BssSI 3081 CACGAG CACGAG(-5/-1)
Bsu36I 3948 CCTGAGG CC^T[ACGT]AGG
CpoI 1125 CGGTCCG CG^G[AT]CCG
Eco81I 3948 CCTGAGG CC^T[ACGT]AGG
EcoRI 1103 GAATTC G^AATTC
EcoRV 1959 GATATC GAT^ATC
EcoT22I 2887 ATGCAT ATGCA^T
FbaI 817 TGATCA T^GATCA
MamI 362 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 823 CAATTG C^AATTG
MunI 823 CAATTG C^AATTG
NdeI 4011 CATATG CA^TATG
NsiI 2887 ATGCAT ATGCA^T
PaeR7I 4143 CTCGAG C^TCGAG
PmaCI 1092 CACGTG CAC^GTG
PmlI 1092 CACGTG CAC^GTG
Psp1406I 920 AACGTT AA^CGTT
RsrII 1125 CGGTCCG CG^G[AT]CCG
SalI 2880 GTCGAC G^TCGAC
SexAI 2621 ACCTGGT A^CC[AT]GGT
SphI 90 GCATGC GCATG^C
XbaI 3494 TCTAGA T^CTAGA
XhoI 4143 CTCGAG C^TCGAG
XmnI 1743 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 1103 GAATTC [GA]^AATT[CT]
3154 AAATTC
Alw44I 1146 GTGCAC G^TGCAC
1899 GTGCAC
ApaLI 1146 GTGCAC G^TGCAC
1899 GTGCAC
ApoI 1103 GAATTC [GA]^AATT[CT]
3154 AAATTC
BamHI 2331 GGATCC G^GATCC
4118 GGATCC
BsaAI 1092 CACGTG [CT]AC^GT[GA]
2043 TACGTG
Bsp1407I 1522 TGTACA T^GTACA
4413 TGTACA
BsrDI 2521 CATTGC GCAATG(2/0)
2890 CATTGC
BsrGI 1522 TGTACA T^GTACA
4413 TGTACA
BssHII 153 GCGCGC G^CGCGC
202 GCGCGC
DraIII 2620 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3435 CACGGTGTG
DrdI 971 GACTGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2871 GACTCTGCTGTC
MslI 212 CACGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2039 CATCTACGTG
NaeI 3801 GCCGGC GCC^GGC
3876 GCCGGC
NgoMIV 3801 GCCGGC G^CCGGC
3876 GCCGGC
NheI 516 GCTAGC G^CTAGC
2442 GCTAGC
NspI 90 GCATGC [GA]CATG^[CT]
2167 ACATGC
PstI 1433 CTGCAG CTGCA^G
3916 CTGCAG
PvuII 235 CAGCTG CAG^CTG
534 CAGCTG
SacI 1772 GAGCTC GAGCT^C
3405 GAGCTC
SapI 1857 GAAGAGC GCTCTTC(1/4)
4333 GCTCTTC
SmaI 242 CCCGGG CCC^GGG
3481 CCCGGG
SspBI 1522 TGTACA T^GTACA
4413 TGTACA
StuI 3342 AGGCCT AGG^CCT
4071 AGGCCT
XcmI 2360 CCAGGGCCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3963 CCAGGCCCGGAATGG
XmaCI 242 CCCGGG C^CCGGG
3481 CCCGGG
XmaI 242 CCCGGG C^CCGGG
3481 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspDI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I ClaI DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII