##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AclI AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BbeI BciVI BclI
BglII BlnI BsaBI BsiEI BsiWI
BsmBI BspHI BspLU11I BssHII Bst1107I
BstBI BstZ17I CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoT22I FbaI
FseI FspI HaeII HincII HindII
HpaI KasI KspI MamI MfeI
MluI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 2855 GGTACC G^GTACC
AccI 3124 GTCTAC GT^[AC][GT]AC
AccIII 2501 TCCGGA T^CCGGA
AflII 547 CTTAAG C^TTAAG
Alw44I 2724 GTGCAC G^TGCAC
ApaLI 2724 GTGCAC G^TGCAC
Asp718I 2855 GGTACC G^GTACC
AspI 2038 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1114 GTCTTC GAAGAC(2/6)
BbvCI 1546 CCTCAGC CCTCAGC(-5/-2)
BcgI 3580 GCAGTGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 547 CTTAAG C^TTAAG
BlpI 3194 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3194 GCTCAGC GC^T[ACGT]AGC
BpuAI 1114 GTCTTC GAAGAC(2/6)
BseAI 2501 TCCGGA T^CCGGA
BspDI 1864 ATCGAT AT^CGAT
BspEI 2501 TCCGGA T^CCGGA
BsrBI 3526 CCGCTC CCGCTC(-3/-3)
BsrDI 2364 CATTGC GCAATG(2/0)
BsrFI 57 GCCGGT [GA]^CCGG[CT]
BssSI 877 CTCGTG CACGAG(-5/-1)
CelII 3194 GCTCAGC GC^T[ACGT]AGC
Cfr10I 57 GCCGGT [GA]^CCGG[CT]
ClaI 1864 ATCGAT AT^CGAT
DraI 345 TTTAAA TTT^AAA
DraIII 2718 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1230 GACCCACACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 278 CCATGG C^C[GA][CT]GG
EcoRV 2918 GATATC GAT^ATC
HindIII 936 AAGCTT A^AGCTT
KpnI 2855 GGTACC GGTAC^C
MroI 2501 TCCGGA T^CCGGA
NcoI 278 CCATGG C^CATGG
NdeI 1208 CATATG CA^TATG
PflFI 2038 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3600 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 344 GTTTAAAC GTTT^AAAC
SacI 1247 GAGCTC GAGCT^C
SbfI 3791 CCTGCAGG CCTGCA^GG
SmaI 2218 CCCGGG CCC^GGG
SpeI 1167 ACTAGT A^CTAGT
Sse8387I 3791 CCTGCAGG CCTGCA^GG
SspI 285 AATATT AAT^ATT
Tth111I 2038 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3600 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3847 TCTAGA T^CTAGA
XmaCI 2218 CCCGGG C^CCGGG
XmaI 2218 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AocI 3242 CCTCAGG CC^T[ACGT]AGG
3347 CCTGAGG
BamHI 2504 GGATCC G^GATCC
3389 GGATCC
BbrPI 736 CACGTG CAC^GTG
2845 CACGTG
BsaAI 736 CACGTG [CT]AC^GT[GA]
2845 CACGTG
BsaI 554 GAGACC GGTCTC(1/5)
3380 GGTCTC
BsaWI 2501 TCCGGA [AT]^CCGG[AT]
2858 ACCGGA
BsmAI 554 GAGAC GTCTC(1/5)
3381 GTCTC
Bsp1407I 2096 TGTACA T^GTACA
2465 TGTACA
BsrGI 2096 TGTACA T^GTACA
2465 TGTACA
BstAPI 2717 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3611 GCACACCCTGC
BstEII 2271 GGTGACC G^GT[ACGT]ACC
3335 GGTGACC
BstPI 2271 GGTGACC G^GT[ACGT]ACC
3335 GGTGACC
BstXI 3404 CCAGCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3544 CCAGGCCCCTGG
Bsu36I 3242 CCTCAGG CC^T[ACGT]AGG
3347 CCTGAGG
Eco81I 3242 CCTCAGG CC^T[ACGT]AGG
3347 CCTGAGG
EcoNI 1519 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1896 CCTGCGCGAGG
EcoO65I 2271 GGTGACC G^GT[ACGT]ACC
3335 GGTGACC
EcoRI 721 GAATTC G^AATTC
1202 GAATTC
PmaCI 736 CACGTG CAC^GTG
2845 CACGTG
PmlI 736 CACGTG CAC^GTG
2845 CACGTG
PshAI 10 GACGCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3338 GACCTCTGTC
SexAI 350 ACCTGGT A^CC[AT]GGT
2342 ACCTGGT
SphI 2531 GCATGC GCATG^C
3470 GCATGC
SspBI 2096 TGTACA T^GTACA
2465 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII