##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AflII AhdI
Aor51HI ApaI AscI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BcgI BclI BfrI BglI BlnI
BsaBI BseAI BsiEI BsiWI BsmI
BspEI BssHII BssSI BstBI CpoI
DraII DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoRI EcoRV
FauI FbaI FseI FspI KasI
KpnI MamI MfeI MluI MroI
MunI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PmeI PpuMI PshAI PvuI RsrII
SacI SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AcyI 27 GGCGTC G[GA]^CG[CT]C
AgeI 73 ACCGGT A^CCGGT
AseI 3228 ATTAAT AT^TAAT
AsnI 3228 ATTAAT AT^TAAT
AvaI 3876 CTCGGG C^[CT]CG[GA]G
BalI 483 TGGCCA TGG^CCA
BbvCI 4549 GCTGAGG CCTCAGC(-5/-2)
BglII 420 AGATCT A^GATCT
BlpI 3626 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3626 GCTCAGC GC^T[ACGT]AGC
BsaHI 27 GGCGTC G[GA]^CG[CT]C
BsaWI 73 ACCGGT [AT]^CCGG[AT]
BsmBI 4480 CGTCTC CGTCTC(1/5)
BsoBI 3876 CTCGGG C^[CT]CG[GA]G
BspDI 394 ATCGAT AT^CGAT
BspHI 624 TCATGA T^CATGA
BspLU11I 1528 ACATGT A^CATGT
BspMI 615 GCAGGT ACCTGC(4/8)
BsrBI 939 CCGCTC CCGCTC(-3/-3)
BsrDI 1415 CATTGC GCAATG(2/0)
BsrFI 73 ACCGGT [GA]^CCGG[CT]
Bst1107I 3524 GTATAC GTA^TAC
BstAPI 4581 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1735 GGTGACC G^GT[ACGT]ACC
BstPI 1735 GGTGACC G^GT[ACGT]ACC
BstXI 4400 CCAACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 420 AGATCT [GA]^GATC[CT]
BstZ17I 3524 GTATAC GTA^TAC
CelII 3626 GCTCAGC GC^T[ACGT]AGC
Cfr10I 73 ACCGGT [GA]^CCGG[CT]
ClaI 394 ATCGAT AT^CGAT
DrdI 2940 GACCTTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2621 CCTCTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1735 GGTGACC G^GT[ACGT]ACC
HaeII 36 GGCGCT [GA]GCGC^[CT]
HgaI 28 GCGTC GACGC(5/10)
Hin1I 27 GGCGTC G[GA]^CG[CT]C
HincII 1840 GTTAAC GT[CT]^[GA]AC
HindII 1840 GTTAAC GT[CT]^[GA]AC
HindIII 2758 AAGCTT A^AGCTT
HpaI 1840 GTTAAC GTT^AAC
KspI 23 CCGCGG CCGC^GG
MflI 420 AGATCT [GA]^GATC[CT]
MluNI 483 TGGCCA TGG^CCA
MscI 483 TGGCCA TGG^CCA
NdeI 100 CATATG CA^TATG
NheI 1308 GCTAGC G^CTAGC
PinAI 73 ACCGGT A^CCGGT
PshBI 3228 ATTAAT AT^TAAT
PstI 2335 CTGCAG CTGCA^G
RcaI 624 TCATGA T^CATGA
SacII 23 CCGCGG CCGC^GG
SapI 172 GAAGAGC GCTCTTC(1/4)
ScaI 1772 AGTACT AGT^ACT
SpeI 2690 ACTAGT A^CTAGT
SphI 2583 GCATGC GCATG^C
StuI 1428 AGGCCT AGG^CCT
XhoII 420 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AclI 1352 AACGTT AA^CGTT
1843 AACGTT
Alw44I 3538 GTGCAC G^TGCAC
4521 GTGCAC
AlwNI 3426 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
4544 CAGAGGCTG
AocI 4172 CCTTAGG CC^T[ACGT]AGG
4433 CCTAAGG
ApaLI 3538 GTGCAC G^TGCAC
4521 GTGCAC
BanI 728 GGCACC G^G[CT][GA]CC
3646 GGCACC
BbrPI 1324 CACGTG CAC^GTG
3833 CACGTG
BciVI 1339 GGATAC GTATCC(6/5)
1257 GTATCC
BsaI 3012 GAGACC GGTCTC(1/5)
1807 GGTCTC
BsgI 311 CTGCAC GTGCAG(16/14)
1327 GTGCAG
Bsp1407I 1455 TGTACA T^GTACA
4066 TGTACA
BsrGI 1455 TGTACA T^GTACA
4066 TGTACA
Bsu36I 4172 CCTTAGG CC^T[ACGT]AGG
4433 CCTAAGG
CfoI 37 GCGC GCG^C
4376 GCGC
DraI 3915 TTTAAA TTT^AAA
4272 TTTAAA
EaeI 430 CGGCCA [CT]^GGCC[GA]
483 TGGCCA
Eco81I 4172 CCTTAGG CC^T[ACGT]AGG
4433 CCTAAGG
EcoT22I 448 ATGCAT ATGCA^T
1542 ATGCAT
HhaI 37 GCGC GCG^C
4376 GCGC
HinP1I 37 GCGC G^CGC
4376 GCGC
NcoI 1476 CCATGG C^CATGG
4046 CCATGG
NsiI 448 ATGCAT ATGCA^T
1542 ATGCAT
PflMI 280 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1798 CCAGTAAGTGG
PmaCI 1324 CACGTG CAC^GTG
3833 CACGTG
PmlI 1324 CACGTG CAC^GTG
3833 CACGTG
Psp1406I 1352 AACGTT AA^CGTT
1843 AACGTT
SspBI 1455 TGTACA T^GTACA
4066 TGTACA
Van91I 280 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1798 CCAGTAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI Asp700I
AspHI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
Cfr13I DdeI DpnII DsaI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI