##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AlwNI ApaI AscI AseI
AsnI Asp718I AviII AvrII BbrPI
BclI BlnI BsaAI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BspHI
BspLU11I BsrBI BssHII Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI DrdI
EagI EclXI Eco52I EcoNI FbaI
FseI FspI HindIII HpaI KpnI
KspI MamI MluI MroI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AflII 1539 CTTAAG C^TTAAG
AhdI 1271 GACGGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1071 AGCGCT AGC^GCT
AspEI 1271 GACGGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3130 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 240 CTCGGG C^[CT]CG[GA]G
BfrI 1539 CTTAAG C^TTAAG
BlpI 721 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 721 GCTGAGC GC^T[ACGT]AGC
BsiEI 307 CGACCG CG[GA][CT]^CG
BsoBI 240 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1161 TGTACA T^GTACA
BsrGI 1161 TGTACA T^GTACA
BssSI 3415 CTCGTG CACGAG(-5/-1)
CelII 721 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1271 GACGGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1071 AGCGCT AGC^GCT
EcoRI 4165 GAATTC G^AATTC
EcoRV 3390 GATATC GAT^ATC
EcoT22I 4220 ATGCAT ATGCA^T
HincII 2644 GTTGAC GT[CT]^[GA]AC
HindII 2644 GTTGAC GT[CT]^[GA]AC
MfeI 613 CAATTG C^AATTG
MunI 613 CAATTG C^AATTG
NdeI 2650 CATATG CA^TATG
NsiI 4220 ATGCAT ATGCA^T
PflFI 3130 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SphI 1059 GCATGC GCATG^C
SspBI 1161 TGTACA T^GTACA
Tth111I 3130 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2660 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 656 CGCG CG^CG
1209 CGCG
Alw44I 2313 GTGCAC G^TGCAC
3027 GTGCAC
ApaLI 2313 GTGCAC G^TGCAC
3027 GTGCAC
BamHI 1622 GGATCC G^GATCC
2244 GGATCC
BbeI 1245 GGCGCC GGCGC^C
2471 GGCGCC
BbvCI 862 CCTCAGC CCTCAGC(-5/-2)
1144 CCTCAGC
BciVI 3626 GGATAC GTATCC(6/5)
2227 GTATCC
BglII 825 AGATCT A^GATCT
999 AGATCT
BsaI 875 GAGACC GGTCTC(1/5)
1222 GGTCTC
BsmBI 3376 CGTCTC CGTCTC(1/5)
890 GAGACG
BsmI 4218 GAATGC GAATGC(1/-1)
4037 GCATTC
BsrFI 480 GCCGGC [GA]^CCGG[CT]
1077 GCCGGC
BstEII 910 GGTGACC G^GT[ACGT]ACC
2711 GGTAACC
BstPI 910 GGTGACC G^GT[ACGT]ACC
2711 GGTAACC
BstUI 656 CGCG CG^CG
1209 CGCG
Cfr10I 480 GCCGGC [GA]^CCGG[CT]
1077 GCCGGC
DraIII 170 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
3219 CACAATGTG
EcoO65I 910 GGTGACC G^GT[ACGT]ACC
2711 GGTAACC
HgaI 47 GACGC GACGC(5/10)
143 GACGC
KasI 1245 GGCGCC G^GCGCC
2471 GGCGCC
MvnI 656 CGCG CG^CG
1209 CGCG
NaeI 480 GCCGGC GCC^GGC
1077 GCCGGC
NarI 1245 GGCGCC GG^CGCC
2471 GGCGCC
NgoMIV 480 GCCGGC G^CCGGC
1077 GCCGGC
NspI 1059 GCATGC [GA]CATG^[CT]
4222 GCATGT
SacI 922 GAGCTC GAGCT^C
1508 GAGCTC
SexAI 408 ACCTGGT A^CC[AT]GGT
438 ACCTGGT
SfoI 1245 GGCGCC GGC^GCC
2471 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AocI ApoI Asp700I
AspHI AvaII BalI BanI BanII
BbsI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI