##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AclI AscI AseI AsnI
Asp718I AspI AvaI AvrII BamHI
BanI BbeI BbrPI BciVI BglII
BlnI BsaAI BsaBI BsiWI BsoBI
BspDI BssHII Bst1107I BstBI BstZ17I
ClaI DraI DrdI EcoT22I FseI
KasI KpnI MamI MfeI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshBI Psp1406I PvuI SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 522 GACGTC GACGT^C
AccIII 4033 TCCGGA T^CCGGA
AgeI 2830 ACCGGT A^CCGGT
Alw44I 5947 GTGCAC G^TGCAC
Aor51HI 1936 AGCGCT AGC^GCT
ApaLI 5947 GTGCAC G^TGCAC
AviII 3538 TGCGCA TGC^GCA
BbvCI 2154 GCTGAGG CCTCAGC(-5/-2)
BclI 3412 TGATCA T^GATCA
BseAI 4033 TCCGGA T^CCGGA
Bsp1407I 329 TGTACA T^GTACA
BspEI 4033 TCCGGA T^CCGGA
BsrGI 329 TGTACA T^GTACA
BstEII 1495 GGTTACC G^GT[ACGT]ACC
BstPI 1495 GGTTACC G^GT[ACGT]ACC
CpoI 5442 CGGTCCG CG^G[AT]CCG
DraIII 642 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 5447 CGGCCG C^GGCCG
EclXI 5447 CGGCCG C^GGCCG
Eco47III 1936 AGCGCT AGC^GCT
Eco52I 5447 CGGCCG C^GGCCG
EcoO65I 1495 GGTTACC G^GT[ACGT]ACC
EcoRV 2475 GATATC GAT^ATC
FbaI 3412 TGATCA T^GATCA
FspI 3538 TGCGCA TGC^GCA
HpaI 4452 GTTAAC GTT^AAC
KspI 5450 CCGCGG CCGC^GG
MluI 2495 ACGCGT A^CGCGT
MroI 4033 TCCGGA T^CCGGA
PinAI 2830 ACCGGT A^CCGGT
PshAI 4107 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 5442 CGGTCCG CG^G[AT]CCG
SacII 5450 CCGCGG CCGC^GG
SbfI 4796 CCTGCAGG CCTGCA^GG
SphI 2212 GCATGC GCATG^C
Sse8387I 4796 CCTGCAGG CCTGCA^GG
SspBI 329 TGTACA T^GTACA
XbaI 1802 TCTAGA T^CTAGA
XcmI 2209 CCAGCATGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 2976 GTCTAC GT^[AC][GT]AC
5967 GTAGAC
AcyI 522 GACGTC G[GA]^CG[CT]C
3111 GACGCC
AflII 1425 CTTAAG C^TTAAG
2546 CTTAAG
AhdI 4119 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5365 GACCCTCTGTC
AocI 2927 CCTGAGG CC^T[ACGT]AGG
5278 CCTCAGG
ApaI 1888 GGGCCC GGGCC^C
5929 GGGCCC
Asp700I 81 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1956 GAAAAGCTTC
AspEI 4119 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5365 GACCCTCTGTC
BfrI 1425 CTTAAG C^TTAAG
2546 CTTAAG
BglI 2163 GCCTTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4438 GCCTTCCTGGC
BsaHI 522 GACGTC G[GA]^CG[CT]C
3111 GACGCC
BsaWI 2830 ACCGGT [AT]^CCGG[AT]
4033 TCCGGA
BsmBI 524 CGTCTC CGTCTC(1/5)
3225 GAGACG
BspLU11I 5080 ACATGT A^CATGT
5769 ACATGT
BsrBI 3965 CCGCTC CCGCTC(-3/-3)
4041 GAGCGG
BsrFI 2830 ACCGGT [GA]^CCGG[CT]
3169 ACCGGC
BstAPI 248 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1763 GCAGATTTTGC
Bsu36I 2927 CCTGAGG CC^T[ACGT]AGG
5278 CCTCAGG
Cfr10I 2830 ACCGGT [GA]^CCGG[CT]
3169 ACCGGC
Eam1105I 4119 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5365 GACCCTCTGTC
Eco81I 2927 CCTGAGG CC^T[ACGT]AGG
5278 CCTCAGG
Hin1I 522 GACGTC G[GA]^CG[CT]C
3111 GACGCC
SspI 45 AATATT AAT^ATT
3636 AATATT
StuI 1333 AGGCCT AGG^CCT
3688 AGGCCT
XmnI 81 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1956 GAAAAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanII BbsI BbvI
BcgI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspMI BsrDI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII