##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccI AccIII AclI AhdI
Aor51HI AscI AspEI AvrII BbeI
BbrPI BcgI BciVI BlnI BsaAI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI KasI KspI
MluI MroI NaeI NarI NgoMIV
NotI NruI PacI PaeR7I PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfoI SgrAI SmaI SnaBI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 3889 GGCGTC G[GA]^CG[CT]C
AflII 5452 CTTAAG C^TTAAG
AgeI 1399 ACCGGT A^CCGGT
Alw44I 1673 GTGCAC G^TGCAC
ApaLI 1673 GTGCAC G^TGCAC
AseI 3991 ATTAAT AT^TAAT
AsnI 3991 ATTAAT AT^TAAT
AspI 2569 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 575 CCCGAG C^[CT]CG[GA]G
BamHI 324 GGATCC G^GATCC
BfrI 5452 CTTAAG C^TTAAG
BglI 574 GCCCGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 202 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 202 GCTGAGC GC^T[ACGT]AGC
BsaHI 3889 GGCGTC G[GA]^CG[CT]C
BsaWI 1399 ACCGGT [AT]^CCGG[AT]
BsoBI 575 CCCGAG C^[CT]CG[GA]G
BsrFI 1399 ACCGGT [GA]^CCGG[CT]
CelII 202 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1399 ACCGGT [GA]^CCGG[CT]
DraI 468 TTTAAA TTT^AAA
DrdI 347 GACATCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 5120 GAATTC G^AATTC
HaeII 3303 AGCGCC [GA]GCGC^[CT]
Hin1I 3889 GGCGTC G[GA]^CG[CT]C
HindIII 863 AAGCTT A^AGCTT
HpaI 4999 GTTAAC GTT^AAC
NdeI 440 CATATG CA^TATG
PflFI 2569 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1399 ACCGGT A^CCGGT
PshBI 3991 ATTAAT AT^TAAT
ScaI 1639 AGTACT AGT^ACT
SfiI 573 GGCCCGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 5422 AGGCCT AGG^CCT
Tth111I 2569 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4560 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 1 CGCG CG^CG
4060 CGCG
ApaI 572 GGGCCC GGGCC^C
2432 GGGCCC
Asp700I 558 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4710 GAAGAGATTC
AviII 1344 TGCGCA TGC^GCA
1739 TGCGCA
BbvCI 2959 CCTCAGC CCTCAGC(-5/-2)
2164 GCTGAGG
BclI 1634 TGATCA T^GATCA
3986 TGATCA
BglII 881 AGATCT A^GATCT
3819 AGATCT
BsaI 851 GAGACC GGTCTC(1/5)
5081 GGTCTC
Bsp1407I 4240 TGTACA T^GTACA
4837 TGTACA
BsrGI 4240 TGTACA T^GTACA
4837 TGTACA
BstAPI 5263 GCACATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5324 GCACTGGATGC
BstBI 695 TTCGAA TT^CGAA
4013 TTCGAA
BstUI 1 CGCG CG^CG
4060 CGCG
BstXI 1766 CCATTAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3966 CCATCCAAATGG
EcoT22I 437 ATGCAT ATGCA^T
1462 ATGCAT
FbaI 1634 TGATCA T^GATCA
3986 TGATCA
FspI 1344 TGCGCA TGC^GCA
1739 TGCGCA
HincII 3801 GTTGAC GT[CT]^[GA]AC
4999 GTTAAC
HindII 3801 GTTGAC GT[CT]^[GA]AC
4999 GTTAAC
MvnI 1 CGCG CG^CG
4060 CGCG
NsiI 437 ATGCAT ATGCA^T
1462 ATGCAT
NspV 695 TTCGAA TT^CGAA
4013 TTCGAA
SacI 205 GAGCTC GAGCT^C
1591 GAGCTC
SfuI 695 TTCGAA TT^CGAA
4013 TTCGAA
SmlI 5452 CTTAAG C^T[CT][GA]AG
5934 CTCAAG
SpeI 913 ACTAGT A^CTAGT
5408 ACTAGT
SphI 1460 GCATGC GCATG^C
1846 GCATGC
SspBI 4240 TGTACA T^GTACA
4837 TGTACA
SspI 1163 AATATT AAT^ATT
1695 AATATT
XcmI 1010 CCAAGCAAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5753 CCAAGGCAGCTCTGG
XmnI 558 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4710 GAAGAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp718I AspHI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaBI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BspLU11I BspMI BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MwoI NciI NcoI NdeII NheI
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII