##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccII AclI AcyI AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AviII AvrII BanII BbeI BbrPI
BbvCI BciVI BglII BlnI BsaHI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BsrGI BssHII BstEII BstPI
BstUI ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI FauI FseI FspI Hin1I
HpaI KasI KspI MluI MvnI
NarI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SfiI SfoI SgrAI SspBI
StuI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 3373 TCCGGA T^CCGGA
AlwNI 3214 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 2133 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 2589 GTGCTC G[AT]GC[AT]^C
AvaI 3316 CCCGGG C^[CT]CG[GA]G
BcgI 2158 GCAATTATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3398 GCCTTGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1602 GCTCAGC GC^T[ACGT]AGC
BmyI 2589 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 1602 GCTCAGC GC^T[ACGT]AGC
BsaAI 2839 TACGTA [CT]AC^GT[GA]
BseAI 3373 TCCGGA T^CCGGA
BseRI 2103 GAGGAG GAGGAG(10/8)
BsiHKAI 2589 GTGCTC G[AT]GC[AT]^C
BsmI 3362 GCATTC GAATGC(1/-1)
BsoBI 3316 CCCGGG C^[CT]CG[GA]G
Bsp1286I 2589 GTGCTC G[AGT]GC[ACT]^C
BspEI 3373 TCCGGA T^CCGGA
BspHI 2612 TCATGA T^CATGA
BspMI 4070 GCAGGT ACCTGC(4/8)
BsrBI 3500 GAGCGG CCGCTC(-3/-3)
BsrDI 374 CATTGC GCAATG(2/0)
BsrFI 3358 GCCGGC [GA]^CCGG[CT]
CelII 1602 GCTCAGC GC^T[ACGT]AGC
CfoI 3407 GCGC GCG^C
Cfr10I 3358 GCCGGC [GA]^CCGG[CT]
DraIII 3279 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 980 ATGCAT ATGCA^T
HaeII 3406 GGCGCT [GA]GCGC^[CT]
HgaI 2752 GACGC GACGC(5/10)
HhaI 3407 GCGC GCG^C
HinP1I 3407 GCGC G^CGC
MroI 3373 TCCGGA T^CCGGA
NaeI 3358 GCCGGC GCC^GGC
NgoMIV 3358 GCCGGC G^CCGGC
NsiI 980 ATGCAT ATGCA^T
PstI 4068 CTGCAG CTGCA^G
RcaI 2612 TCATGA T^CATGA
SbfI 4067 CCTGCAGG CCTGCA^GG
SexAI 970 ACCTGGT A^CC[AT]GGT
SmaI 3316 CCCGGG CCC^GGG
SnaBI 2839 TACGTA TAC^GTA
SpeI 3417 ACTAGT A^CTAGT
SphI 2426 GCATGC GCATG^C
Sse8387I 4067 CCTGCAGG CCTGCA^GG
XmaCI 3316 CCCGGG C^CCGGG
XmaI 3316 CCCGGG C^CCGGG
XmnI 2133 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 1812 GGTACC G^GTACC
3882 GGTACC
AflII 321 CTTAAG C^TTAAG
3647 CTTAAG
AocI 576 CCTCAGG CC^T[ACGT]AGG
3490 CCTCAGG
Asp718I 1812 GGTACC G^GTACC
3882 GGTACC
BalI 746 TGGCCA TGG^CCA
1892 TGGCCA
BclI 1351 TGATCA T^GATCA
2915 TGATCA
BcnI 3316 CCCGG CC^[GC]GG
3317 CCGGG
BfrI 321 CTTAAG C^TTAAG
3647 CTTAAG
BsaBI 168 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1800 GATAGAAATC
BsaI 1467 GAGACC GGTCTC(1/5)
567 GGTCTC
BsaWI 3373 TCCGGA [AT]^CCGG[AT]
3781 TCCGGT
BssSI 219 CTCGTG CACGAG(-5/-1)
3582 CTCGTG
Bst1107I 1064 GTATAC GTA^TAC
1547 GTATAC
BstBI 1189 TTCGAA TT^CGAA
3433 TTCGAA
BstZ17I 1064 GTATAC GTA^TAC
1547 GTATAC
Bsu36I 576 CCTCAGG CC^T[ACGT]AGG
3490 CCTCAGG
DraI 728 TTTAAA TTT^AAA
2436 TTTAAA
DraII 2644 GGGACCC [GA]G^G[ACGT]CC[CT]
3002 GGGTCCT
DsaI 1131 CCATGG C^C[GA][CT]GG
1563 CCATGG
EaeI 746 TGGCCA [CT]^GGCC[GA]
1892 TGGCCA
Eco81I 576 CCTCAGG CC^T[ACGT]AGG
3490 CCTCAGG
EcoO109I 2644 GGGACCC [GA]G^G[ACGT]CC[CT]
3002 GGGTCCT
EcoRV 267 GATATC GAT^ATC
3425 GATATC
FbaI 1351 TGATCA T^GATCA
2915 TGATCA
HincII 1822 GTCAAC GT[CT]^[GA]AC
3041 GTCAAC
HindII 1822 GTCAAC GT[CT]^[GA]AC
3041 GTCAAC
HindIII 1456 AAGCTT A^AGCTT
1512 AAGCTT
KpnI 1812 GGTACC GGTAC^C
3882 GGTACC
MaeII 2840 ACGT A^CGT
3175 ACGT
MamI 168 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1800 GATAGAAATC
MfeI 612 CAATTG C^AATTG
1306 CAATTG
MluNI 746 TGGCCA TGG^CCA
1892 TGGCCA
MscI 746 TGGCCA TGG^CCA
1892 TGGCCA
MspA1I 117 CAGCTG C[AC]G^C[GT]G
302 CAGCTG
MunI 612 CAATTG C^AATTG
1306 CAATTG
NciI 3316 CCCGG CC^[GC]GG
3317 CCGGG
NcoI 1131 CCATGG C^CATGG
1563 CCATGG
NheI 1943 GCTAGC G^CTAGC
2111 GCTAGC
NspV 1189 TTCGAA TT^CGAA
3433 TTCGAA
PflMI 2213 CCACCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3701 CCAAATTGTGG
PpuMI 2644 GGGACCC [GA]G^G[AT]CC[CT]
3002 GGGTCCT
PshAI 3813 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4152 GACTCCTGTC
PvuII 117 CAGCTG CAG^CTG
302 CAGCTG
SfuI 1189 TTCGAA TT^CGAA
3433 TTCGAA
Van91I 2213 CCACCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3701 CCAAATTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspI AvaII BamHI
BanI BbsI BbvI BfaI BpmI
BpuAI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoNI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeI NdeII NlaIII
NlaIV NspI PflFI PleI RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XcmI
XhoII