##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII BbrPI BciVI BfrI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BssHII Bst1107I BstZ17I ClaI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FseI FspI HindIII HpaI KpnI
MluI MroI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
SalI SbfI SfiI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AocI 3539 CCTGAGG CC^T[ACGT]AGG
Asp700I 4228 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3855 CCTAGG C^CTAGG
BamHI 393 GGATCC G^GATCC
BbeI 8 GGCGCC GGCGC^C
BcgI 1335 GCAAGATTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2472 TGATCA T^GATCA
BlnI 3855 CCTAGG C^CTAGG
BlpI 1846 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1846 GCTCAGC GC^T[ACGT]AGC
BsaBI 1186 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2671 ACCGGA [AT]^CCGG[AT]
BsmBI 2032 CGTCTC CGTCTC(1/5)
Bsp1407I 4517 TGTACA T^GTACA
BsrGI 4517 TGTACA T^GTACA
BssSI 1362 CTCGTG CACGAG(-5/-1)
BstBI 972 TTCGAA TT^CGAA
BstEII 4580 GGTGACC G^GT[ACGT]ACC
BstPI 4580 GGTGACC G^GT[ACGT]ACC
Bsu36I 3539 CCTGAGG CC^T[ACGT]AGG
CelII 1846 GCTCAGC GC^T[ACGT]AGC
CpoI 138 CGGTCCG CG^G[AT]CCG
DraI 4243 TTTAAA TTT^AAA
Eco81I 3539 CCTGAGG CC^T[ACGT]AGG
EcoO65I 4580 GGTGACC G^GT[ACGT]ACC
EcoRI 4156 GAATTC G^AATTC
FbaI 2472 TGATCA T^GATCA
KasI 8 GGCGCC G^GCGCC
KspI 1862 CCGCGG CCGC^GG
MamI 1186 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3637 CAATTG C^AATTG
MunI 3637 CAATTG C^AATTG
NaeI 3683 GCCGGC GCC^GGC
NarI 8 GGCGCC GG^CGCC
NdeI 4634 CATATG CA^TATG
NgoMIV 3683 GCCGGC G^CCGGC
NheI 1675 GCTAGC G^CTAGC
NspV 972 TTCGAA TT^CGAA
RsrII 138 CGGTCCG CG^G[AT]CCG
SacII 1862 CCGCGG CCGC^GG
ScaI 438 AGTACT AGT^ACT
SexAI 2819 ACCTGGT A^CC[AT]GGT
SfoI 8 GGCGCC GGC^GCC
SfuI 972 TTCGAA TT^CGAA
SspBI 4517 TGTACA T^GTACA
XmnI 4228 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AatII 593 GACGTC GACGT^C
1123 GACGTC
AccI 4125 GTCTAC GT^[AC][GT]AC
4483 GTCTAC
AcsI 4156 GAATTC [GA]^AATT[CT]
4248 AAATTC
ApoI 4156 GAATTC [GA]^AATT[CT]
4248 AAATTC
BbsI 215 GAAGAC GAAGAC(2/6)
328 GAAGAC
BpuAI 215 GAAGAC GAAGAC(2/6)
328 GAAGAC
BsaI 1254 GAGACC GGTCTC(1/5)
1295 GAGACC
BsgI 254 GTGCAG GTGCAG(16/14)
457 GTGCAG
BsrBI 4047 CCGCTC CCGCTC(-3/-3)
3883 GAGCGG
BsrDI 4394 CATTGC GCAATG(2/0)
1893 GCAATG
DraIII 248 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2146 CACCCAGTG
DrdI 4267 GACCTTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4366 GACCGCAGTGTC
EcoNI 2366 CCTTACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3275 CCTCTTGCAGG
EcoT22I 3742 ATGCAT ATGCA^T
4456 ATGCAT
HaeII 8 GGCGCC [GA]GCGC^[CT]
3105 AGCGCC
NsiI 3742 ATGCAT ATGCA^T
4456 ATGCAT
XcmI 12 CCAATGCCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2826 CCAGCGAGGAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BglII BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PshAI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII
XmaCI XmaI