##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
AseI AsnI Asp718I BglII BsiWI
BspDI BspHI Bst1107I BstBI BstZ17I
ClaI EcoRI EcoRV EcoT22I FseI
HpaI KpnI MfeI MluI MunI
NaeI NgoMIV NotI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI SalI SfuI
SgrAI SnaBI SpeI SwaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcsI 732 AAATTT [GA]^AATT[CT]
AflIII 617 ACATGT A^C[GA][CT]GT
AhdI 7914 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1107 GTGCAC G^TGCAC
AocI 4553 CCTCAGG CC^T[ACGT]AGG
ApaLI 1107 GTGCAC G^TGCAC
ApoI 732 AAATTT [GA]^AATT[CT]
AspEI 7914 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 7159 CCTAGG C^CTAGG
BbrPI 3219 CACGTG CAC^GTG
BciVI 2206 GTATCC GTATCC(6/5)
BclI 6045 TGATCA T^GATCA
BlnI 7159 CCTAGG C^CTAGG
BlpI 1712 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1712 GCTCAGC GC^T[ACGT]AGC
BsaAI 3219 CACGTG [CT]AC^GT[GA]
BsiEI 295 CGGCCG CG[GA][CT]^CG
BspLU11I 617 ACATGT A^CATGT
BssHII 131 GCGCGC G^CGCGC
BstEII 5730 GGTGACC G^GT[ACGT]ACC
BstPI 5730 GGTGACC G^GT[ACGT]ACC
Bsu36I 4553 CCTCAGG CC^T[ACGT]AGG
CelII 1712 GCTCAGC GC^T[ACGT]AGC
CpoI 290 CGGTCCG CG^G[AT]CCG
EagI 295 CGGCCG C^GGCCG
Eam1105I 7914 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 295 CGGCCG C^GGCCG
Eco52I 295 CGGCCG C^GGCCG
Eco81I 4553 CCTCAGG CC^T[ACGT]AGG
EcoO65I 5730 GGTGACC G^GT[ACGT]ACC
FbaI 6045 TGATCA T^GATCA
HindIII 704 AAGCTT A^AGCTT
KspI 141 CCGCGG CCGC^GG
NdeI 859 CATATG CA^TATG
NheI 6917 GCTAGC G^CTAGC
NruI 226 TCGCGA TCG^CGA
PmaCI 3219 CACGTG CAC^GTG
PmlI 3219 CACGTG CAC^GTG
RsrII 290 CGGTCCG CG^G[AT]CCG
SacII 141 CCGCGG CCGC^GG
SbfI 2376 CCTGCAGG CCTGCA^GG
SexAI 4655 ACCAGGT A^CC[AT]GGT
SfiI 287 GGCCGGTCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3464 GCATGC GCATG^C
Sse8387I 2376 CCTGCAGG CCTGCA^GG
SspI 5115 AATATT AAT^ATT
XbaI 5636 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflII 2646 CTTAAG C^TTAAG
7179 CTTAAG
Aor51HI 4856 AGCGCT AGC^GCT
7231 AGCGCT
Asp700I 1566 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2562 GAACCAGTTC
BamHI 476 GGATCC G^GATCC
3854 GGATCC
BcgI 3879 CGACCACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1118 GCAATGGCATCG
BfrI 2646 CTTAAG C^TTAAG
7179 CTTAAG
BsaBI 1905 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7256 GATTGGAATC
BsaI 1177 GAGACC GGTCTC(1/5)
3831 GGTCTC
DraI 1933 TTTAAA TTT^AAA
5012 TTTAAA
DrdI 2476 GACACCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2817 GACCTCCCTGTC
Eco47III 4856 AGCGCT AGC^GCT
7231 AGCGCT
MamI 1905 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7256 GATTGGAATC
PshAI 2585 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
7346 GACCTGAGTC
SacI 301 GAGCTC GAGCT^C
6746 GAGCTC
SapI 147 GCTCTTC GCTCTTC(1/4)
963 GCTCTTC
ScaI 1727 AGTACT AGT^ACT
5316 AGTACT
XmnI 1566 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2562 GAACCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccI AccII AccIII AciI AcyI
AfaI AluI AlwI AlwNI ApaI
AspHI AspI AvaI AvaII AviII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspEI
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI