##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AclI AflIII AscI
AseI AsnI Asp718I BglII BsiWI
BspDI BspHI BspLU11I BsrBI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoO65I EcoRV EcoT22I HindIII
HpaI KpnI MfeI MluI MunI
NheI NotI NruI NsiI NspV
PacI PaeR7I PmeI PshBI Psp1406I
RcaI RsrII SfiI SfuI SgrAI
SnaBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 2744 GTCGAC GT^[AC][GT]AC
AgeI 5192 ACCGGT A^CCGGT
Aor51HI 5004 AGCGCT AGC^GCT
Asp700I 1584 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 5603 TGCGCA TGC^GCA
AvrII 5289 CCTAGG C^CTAGG
BamHI 54 GGATCC G^GATCC
BbrPI 4019 CACGTG CAC^GTG
BcgI 1398 CGAGCCCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5669 GGATAC GTATCC(6/5)
BlnI 5289 CCTAGG C^CTAGG
BsaAI 4019 CACGTG [CT]AC^GT[GA]
BsaBI 262 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3438 CGATCG CG[GA][CT]^CG
BsmBI 3158 GAGACG CGTCTC(1/5)
Bsp1407I 4638 TGTACA T^GTACA
BsrDI 5905 GCAATG GCAATG(2/0)
BsrGI 4638 TGTACA T^GTACA
BssHII 3250 GCGCGC G^CGCGC
Eco47III 5004 AGCGCT AGC^GCT
FseI 2113 GGCCGGCC GGCCGG^CC
FspI 5603 TGCGCA TGC^GCA
KspI 1718 CCGCGG CCGC^GG
MamI 262 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 4317 CATATG CA^TATG
PinAI 5192 ACCGGT A^CCGGT
PmaCI 4019 CACGTG CAC^GTG
PmlI 4019 CACGTG CAC^GTG
PshAI 663 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3438 CGATCG CGAT^CG
SacII 1718 CCGCGG CCGC^GG
SalI 2744 GTCGAC G^TCGAC
SbfI 1575 CCTGCAGG CCTGCA^GG
ScaI 5555 AGTACT AGT^ACT
SpeI 5535 ACTAGT A^CTAGT
SphI 1748 GCATGC GCATG^C
Sse8387I 1575 CCTGCAGG CCTGCA^GG
SspBI 4638 TGTACA T^GTACA
XbaI 5087 TCTAGA T^CTAGA
XmnI 1584 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 148 TCCGGA T^CCGGA
4722 TCCGGA
AflII 2494 CTTAAG C^TTAAG
2791 CTTAAG
AhdI 283 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2227 GACGACAAGTC
Alw44I 79 GTGCAC G^TGCAC
616 GTGCAC
AlwNI 1780 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
4944 CAGAAGCTG
ApaLI 79 GTGCAC G^TGCAC
616 GTGCAC
AspEI 283 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2227 GACGACAAGTC
AspI 2365 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3990 GACCTGGTC
BclI 267 TGATCA T^GATCA
3936 TGATCA
BfrI 2494 CTTAAG C^TTAAG
2791 CTTAAG
BseAI 148 TCCGGA T^CCGGA
4722 TCCGGA
BsmI 2433 GAATGC GAATGC(1/-1)
5180 GAATGC
BspEI 148 TCCGGA T^CCGGA
4722 TCCGGA
DraI 5512 TTTAAA TTT^AAA
5980 TTTAAA
Eam1105I 283 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2227 GACGACAAGTC
EcoRI 4251 GAATTC G^AATTC
4405 GAATTC
FbaI 267 TGATCA T^GATCA
3936 TGATCA
HincII 2744 GTCGAC GT[CT]^[GA]AC
5546 GTTGAC
HindII 2744 GTCGAC GT[CT]^[GA]AC
5546 GTTGAC
MroI 148 TCCGGA T^CCGGA
4722 TCCGGA
NspI 1748 GCATGC [GA]CATG^[CT]
5762 ACATGC
PflFI 2365 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3990 GACCTGGTC
Tth111I 2365 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3990 GACCTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI