##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AccIII AgeI AhdI AocI
Aor51HI AscI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbvCI BcgI BlnI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BsrBI
BssHII Bsu36I ClaI CpoI Eam1105I
Eco47III Eco81I FseI FspI KasI
KpnI KspI MluNI MroI MscI
NarI NotI NruI PacI PflFI
PinAI PmeI PshAI PvuI RsrII
SacII SapI SbfI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3 GACGTC GACGT^C
AcyI 3 GACGTC G[GA]^CG[CT]C
Alw44I 5942 GTGCAC G^TGCAC
ApaI 2619 GGGCCC GGGCC^C
ApaLI 5942 GTGCAC G^TGCAC
BanI 2359 GGCACC G^G[CT][GA]CC
BbrPI 512 CACGTG CAC^GTG
BclI 2166 TGATCA T^GATCA
BsaBI 6234 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3 GACGTC G[GA]^CG[CT]C
BsiEI 2473 CGGCCG CG[GA][CT]^CG
BsmI 2840 GCATTC GAATGC(1/-1)
Bsp1407I 5787 TGTACA T^GTACA
BsrGI 5787 TGTACA T^GTACA
BstBI 2286 TTCGAA TT^CGAA
BstXI 1458 CCAAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 2473 CGGCCG C^GGCCG
EclXI 2473 CGGCCG C^GGCCG
Eco52I 2473 CGGCCG C^GGCCG
EcoRI 4643 GAATTC G^AATTC
EcoRV 581 GATATC GAT^ATC
FbaI 2166 TGATCA T^GATCA
HgaI 182 GACGC GACGC(5/10)
Hin1I 3 GACGTC G[GA]^CG[CT]C
MamI 6234 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 183 ACGCGT A^CGCGT
NdeI 3362 CATATG CA^TATG
NheI 694 GCTAGC G^CTAGC
NspV 2286 TTCGAA TT^CGAA
PflMI 13 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 512 CACGTG CAC^GTG
PmlI 512 CACGTG CAC^GTG
SalI 1955 GTCGAC G^TCGAC
ScaI 5038 AGTACT AGT^ACT
SfuI 2286 TTCGAA TT^CGAA
SpeI 5905 ACTAGT A^CTAGT
SspBI 5787 TGTACA T^GTACA
Van91I 13 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AclI 2174 AACGTT AA^CGTT
3878 AACGTT
BglI 117 GCCCCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
881 GCCATGCTGGC
BsgI 3301 CTGCAC GTGCAG(16/14)
5152 GTGCAG
BspMI 957 ACCTGC ACCTGC(4/8)
2400 GCAGGT
BsrFI 113 GCCGGC [GA]^CCGG[CT]
254 GCCGGC
BssSI 622 CACGAG CACGAG(-5/-1)
3275 CACGAG
BstAPI 362 GCAGAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3303 GCACTGTTTGC
BstEII 2403 GGTCACC G^GT[ACGT]ACC
4391 GGTTACC
BstPI 2403 GGTCACC G^GT[ACGT]ACC
4391 GGTTACC
Cfr10I 113 GCCGGC [GA]^CCGG[CT]
254 GCCGGC
DraIII 2442 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
5945 CACTGTGTG
DrdI 101 GACCAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1568 GACATTCAAGTC
EcoO65I 2403 GGTCACC G^GT[ACGT]ACC
4391 GGTTACC
FauI 160 CCCGC CCCGC(4/6)
2623 CCCGC
HindIII 1762 AAGCTT A^AGCTT
3381 AAGCTT
HpaI 4181 GTTAAC GTT^AAC
4472 GTTAAC
MfeI 2060 CAATTG C^AATTG
4922 CAATTG
MunI 2060 CAATTG C^AATTG
4922 CAATTG
NaeI 113 GCCGGC GCC^GGC
254 GCCGGC
NcoI 2049 CCATGG C^CATGG
2986 CCATGG
NgoMIV 113 GCCGGC G^CCGGC
254 GCCGGC
PaeR7I 272 CTCGAG C^TCGAG
303 CTCGAG
Psp1406I 2174 AACGTT AA^CGTT
3878 AACGTT
PvuII 104 CAGCTG CAG^CTG
1960 CAGCTG
SexAI 1548 ACCAGGT A^CC[AT]GGT
3034 ACCAGGT
SphI 5157 GCATGC GCATG^C
5396 GCATGC
StuI 2609 AGGCCT AGG^CCT
5168 AGGCCT
XcmI 13 CCAGGACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4019 CCAGAAGCCAACTGG
XhoI 272 CTCGAG C^TCGAG
303 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AflIII AluI AlwI AlwNI
ApoI AseI AsnI Asp700I AspHI
AvaI AvaII BanII BbsI BbvI
BciVI BcnI BfaI BfrI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspLU11I BsrDI BsrI
BssKI Bst1107I BstF5I BstNI BstUI
BstYI BstZ17I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI PshBI
PstI RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI