##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII Acc65I AclI AflII Aor51HI
AscI Asp700I Asp718I AviII AvrII
BbeI BfrI BlnI BsaBI BsiWI
BsmBI BspDI BssHII BstBI ClaI
CpoI Eco47III FseI FspI HindIII
KasI KpnI MamI MfeI MluI
MunI NaeI NarI NgoMIV NruI
NspV PaeR7I PmeI Psp1406I PvuI
RsrII SalI SbfI ScaI SfoI
SfuI SnaBI SpeI Sse8387I SspI
SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 5969 GTATAC GT^[AC][GT]AC
AccIII 2201 TCCGGA T^CCGGA
AseI 4534 ATTAAT AT^TAAT
AsnI 4534 ATTAAT AT^TAAT
BamHI 3121 GGATCC G^GATCC
BclI 1148 TGATCA T^GATCA
BglII 3596 AGATCT A^GATCT
BseAI 2201 TCCGGA T^CCGGA
BsiEI 363 CGGCCG CG[GA][CT]^CG
Bsp1407I 5915 TGTACA T^GTACA
BspEI 2201 TCCGGA T^CCGGA
BspLU11I 6157 ACATGT A^CATGT
BsrGI 5915 TGTACA T^GTACA
BssSI 1436 CACGAG CACGAG(-5/-1)
Bst1107I 5969 GTATAC GTA^TAC
BstZ17I 5969 GTATAC GTA^TAC
DraIII 1436 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3026 GACTGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 363 CGGCCG C^GGCCG
EclXI 363 CGGCCG C^GGCCG
Eco52I 363 CGGCCG C^GGCCG
EcoRV 6060 GATATC GAT^ATC
FbaI 1148 TGATCA T^GATCA
HpaI 2940 GTTAAC GTT^AAC
KspI 4522 CCGCGG CCGC^GG
MroI 2201 TCCGGA T^CCGGA
NheI 152 GCTAGC G^CTAGC
NotI 362 GCGGCCGC GC^GGCCGC
PacI 6219 TTAATTAA TTAAT^TAA
PshAI 5185 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4534 ATTAAT AT^TAAT
SacII 4522 CCGCGG CCGC^GG
SapI 4028 GCTCTTC GCTCTTC(1/4)
SexAI 632 ACCTGGT A^CC[AT]GGT
SfiI 390 GGCCAGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 6104 CACCGGTG C[GA]^CCGG[CT]G
SspBI 5915 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 427 GACGCC G[GA]^CG[CT]C
1579 GACGCC
AflIII 4079 ACACGT A^C[GA][CT]GT
6157 ACATGT
AgeI 5481 ACCGGT A^CCGGT
6105 ACCGGT
AhdI 2359 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2422 GACCCATAGTC
AspEI 2359 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2422 GACCCATAGTC
AspI 2717 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4614 GACAGAGTC
BbrPI 199 CACGTG CAC^GTG
3266 CACGTG
BcgI 663 CGACTTGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4212 CGATCTTTTTGC
BciVI 907 GGATAC GTATCC(6/5)
5585 GGATAC
BsaHI 427 GACGCC G[GA]^CG[CT]C
1579 GACGCC
BsmI 2889 GAATGC GAATGC(1/-1)
3288 GCATTC
BspHI 1151 TCATGA T^CATGA
4132 TCATGA
BsrFI 5481 ACCGGT [GA]^CCGG[CT]
6105 ACCGGT
BstEII 1803 GGTCACC G^GT[ACGT]ACC
4789 GGTCACC
BstPI 1803 GGTCACC G^GT[ACGT]ACC
4789 GGTCACC
Cfr10I 5481 ACCGGT [GA]^CCGG[CT]
6105 ACCGGT
Eam1105I 2359 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2422 GACCCATAGTC
EcoO65I 1803 GGTCACC G^GT[ACGT]ACC
4789 GGTCACC
EcoT22I 2214 ATGCAT ATGCA^T
4970 ATGCAT
HaeII 272 AGCGCC [GA]GCGC^[CT]
4266 AGCGCC
Hin1I 427 GACGCC G[GA]^CG[CT]C
1579 GACGCC
NdeI 698 CATATG CA^TATG
5260 CATATG
NsiI 2214 ATGCAT ATGCA^T
4970 ATGCAT
PflFI 2717 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4614 GACAGAGTC
PinAI 5481 ACCGGT A^CCGGT
6105 ACCGGT
PmaCI 199 CACGTG CAC^GTG
3266 CACGTG
PmlI 199 CACGTG CAC^GTG
3266 CACGTG
RcaI 1151 TCATGA T^CATGA
4132 TCATGA
SmaI 64 CCCGGG CCC^GGG
3125 CCCGGG
SphI 987 GCATGC GCATG^C
4294 GCATGC
Tth111I 2717 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4614 GACAGAGTC
XbaI 3948 TCTAGA T^CTAGA
4939 TCTAGA
XmaCI 64 CCCGGG C^CCGGG
3125 CCCGGG
XmaI 64 CCCGGG C^CCGGG
3125 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII