##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
Aor51HI ApaLI AscI Asp718I AviII
BamHI BbeI BbrPI BciVI BclI
BglI BsaAI BsaWI BseAI BsiWI
BspDI BspEI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraIII
DrdI Eco47III EcoO65I FbaI FseI
FspI HaeII HincII HindII HpaI
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 850 GTCTAC GT^[AC][GT]AC
AccII 5493 CGCG CG^CG
AclI 1464 AACGTT AA^CGTT
AcyI 5707 GACGCC G[GA]^CG[CT]C
AflII 4207 CTTAAG C^TTAAG
AhdI 910 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 910 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2468 TGGCCA TGG^CCA
BanI 3718 GGCACC G^G[CT][GA]CC
BcgI 5660 GCAGGAGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4207 CTTAAG C^TTAAG
BsaBI 2254 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5707 GACGCC G[GA]^CG[CT]C
BsmBI 5746 CGTCTC CGTCTC(1/5)
BsmI 2591 GCATTC GAATGC(1/-1)
BspHI 1304 TCATGA T^CATGA
BstAPI 431 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 5493 CGCG CG^CG
DsaI 127 CCATGG C^C[GA][CT]GG
EagI 5475 CGGCCG C^GGCCG
Eam1105I 910 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5475 CGGCCG C^GGCCG
Eco52I 5475 CGGCCG C^GGCCG
EcoRV 595 GATATC GAT^ATC
EcoT22I 2589 ATGCAT ATGCA^T
FauI 3374 CCCGC CCCGC(4/6)
Hin1I 5707 GACGCC G[GA]^CG[CT]C
MamI 2254 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 151 CAATTG C^AATTG
MluNI 2468 TGGCCA TGG^CCA
MscI 2468 TGGCCA TGG^CCA
MunI 151 CAATTG C^AATTG
MvnI 5493 CGCG CG^CG
NcoI 127 CCATGG C^CATGG
NdeI 261 CATATG CA^TATG
NsiI 2589 ATGCAT ATGCA^T
PshAI 5400 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1464 AACGTT AA^CGTT
RcaI 1304 TCATGA T^CATGA
SacI 1337 GAGCTC GAGCT^C
ScaI 5293 AGTACT AGT^ACT
SmaI 5678 CCCGGG CCC^GGG
SphI 354 GCATGC GCATG^C
XmaCI 5678 CCCGGG C^CCGGG
XmaI 5678 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 946 ACATGT A^C[GA][CT]GT
3359 ACATGT
AlwI 740 GATCC GGATC(4/5)
3835 GATCC
AocI 270 CCTGAGG CC^T[ACGT]AGG
3691 CCTAAGG
ApaI 874 GGGCCC GGGCC^C
5675 GGGCCC
Asp700I 3773 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5429 GAATTCATTC
AspHI 1337 GAGCTC G[AT]GC[AT]^C
3868 GTGCTC
AspI 4017 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4407 GACAATGTC
BlpI 5208 GCTCAGC GC^T[ACGT]AGC
5271 GCTGAGC
Bpu1102I 5208 GCTCAGC GC^T[ACGT]AGC
5271 GCTGAGC
BsaI 5555 GAGACC GGTCTC(1/5)
766 GGTCTC
BsiEI 5475 CGGCCG CG[GA][CT]^CG
5542 CGGTCG
BsiHKAI 1337 GAGCTC G[AT]GC[AT]^C
3868 GTGCTC
Bsp1407I 963 TGTACA T^GTACA
971 TGTACA
BspLU11I 946 ACATGT A^CATGT
3359 ACATGT
BsrGI 963 TGTACA T^GTACA
971 TGTACA
Bsu36I 270 CCTGAGG CC^T[ACGT]AGG
3691 CCTAAGG
CelII 5208 GCTCAGC GC^T[ACGT]AGC
5271 GCTGAGC
DraII 1394 AGGACCC [GA]G^G[ACGT]CC[CT]
4015 GGGACCC
EaeI 2468 TGGCCA [CT]^GGCC[GA]
5475 CGGCCG
Eco81I 270 CCTGAGG CC^T[ACGT]AGG
3691 CCTAAGG
EcoNI 2116 CCTCTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4020 CCTGTCCTAGG
EcoO109I 1394 AGGACCC [GA]G^G[ACGT]CC[CT]
4015 GGGACCC
HgaI 5707 GACGC GACGC(5/10)
643 GCGTC
PflFI 4017 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4407 GACAATGTC
PpuMI 1394 AGGACCC [GA]G^G[AT]CC[CT]
4015 GGGACCC
SapI 1334 GAAGAGC GCTCTTC(1/4)
1488 GAAGAGC
SmlI 3916 CTCAAG C^T[CT][GA]AG
4207 CTTAAG
SpeI 2360 ACTAGT A^CTAGT
5378 ACTAGT
SspBI 963 TGTACA T^GTACA
971 TGTACA
StuI 400 AGGCCT AGG^CCT
5579 AGGCCT
Tth111I 4017 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4407 GACAATGTC
XbaI 4678 TCTAGA T^CTAGA
5153 TCTAGA
XmnI 3773 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5429 GAATTCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AseI AsnI AvaI AvaII
AvrII BanII BbsI BbvCI BbvI
BcnI BfaI BglII BlnI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII