##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AccI AccII AgeI Aor51HI AscI
AspI AviII AvrII BbeI BbrPI
BcgI BcnI BglI BlnI BsaBI
BsiEI BsiWI BspDI BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I EcoNI FauI
FseI FspI KasI KspI MamI
MluI MvnI NarI NciI NcoI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SpeI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 2646 GACGTC GACGT^C
Acc65I 2887 GGTACC G^GTACC
AccIII 2593 TCCGGA T^CCGGA
AclI 4526 AACGTT AA^CGTT
AcyI 2646 GACGTC G[GA]^CG[CT]C
AflII 859 CTTAAG C^TTAAG
AhdI 634 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2353 CCTGAGG CC^T[ACGT]AGG
ApaI 170 GGGCCC GGGCC^C
Asp718I 2887 GGTACC G^GTACC
AspEI 634 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2512 CCCGAG C^[CT]CG[GA]G
BalI 668 TGGCCA TGG^CCA
BbvCI 872 CCTCAGC CCTCAGC(-5/-2)
BfrI 859 CTTAAG C^TTAAG
BsaHI 2646 GACGTC G[GA]^CG[CT]C
BsaI 2305 GAGACC GGTCTC(1/5)
BsaWI 2593 TCCGGA [AT]^CCGG[AT]
BseAI 2593 TCCGGA T^CCGGA
BsmBI 4368 CGTCTC CGTCTC(1/5)
BsoBI 2512 CCCGAG C^[CT]CG[GA]G
BspEI 2593 TCCGGA T^CCGGA
BspHI 366 TCATGA T^CATGA
BstAPI 4159 GCAATTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2353 CCTGAGG CC^T[ACGT]AGG
DrdI 2597 GACTGTCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2699 CCGTGG C^C[GA][CT]GG
EaeI 668 TGGCCA [CT]^GGCC[GA]
Eam1105I 634 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2353 CCTGAGG CC^T[ACGT]AGG
EcoT22I 4855 ATGCAT ATGCA^T
HgaI 3534 GACGC GACGC(5/10)
Hin1I 2646 GACGTC G[GA]^CG[CT]C
KpnI 2887 GGTACC GGTAC^C
MluNI 668 TGGCCA TGG^CCA
MroI 2593 TCCGGA T^CCGGA
MscI 668 TGGCCA TGG^CCA
NaeI 2345 GCCGGC GCC^GGC
NdeI 2705 CATATG CA^TATG
NgoMIV 2345 GCCGGC G^CCGGC
NsiI 4855 ATGCAT ATGCA^T
PpuMI 2767 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 4526 AACGTT AA^CGTT
RcaI 366 TCATGA T^CATGA
SacI 3157 GAGCTC GAGCT^C
ScaI 1657 AGTACT AGT^ACT
SnaBI 5240 TACGTA TAC^GTA
SphI 4377 GCATGC GCATG^C
XbaI 2328 TCTAGA T^CTAGA
XcmI 929 CCAAACATACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 898 GTGCAC G^TGCAC
5273 GTGCAC
ApaLI 898 GTGCAC G^TGCAC
5273 GTGCAC
BanI 2887 GGTACC G^G[CT][GA]CC
3091 GGTGCC
BclI 1599 TGATCA T^GATCA
4554 TGATCA
BlpI 3055 GCTGAGC GC^T[ACGT]AGC
5190 GCTTAGC
Bpu1102I 3055 GCTGAGC GC^T[ACGT]AGC
5190 GCTTAGC
BseRI 904 CTCCTC GAGGAG(10/8)
1469 GAGGAG
BsrFI 527 ACCGGC [GA]^CCGG[CT]
2345 GCCGGC
BstXI 659 CCAACTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3674 CCAAACCAATGG
CelII 3055 GCTGAGC GC^T[ACGT]AGC
5190 GCTTAGC
CfoI 2025 GCGC GCG^C
2730 GCGC
Cfr10I 527 ACCGGC [GA]^CCGG[CT]
2345 GCCGGC
EcoRV 1450 GATATC GAT^ATC
3469 GATATC
EcoT14I 1694 CCTTGG C^C[AT][AT]GG
2906 CCTTGG
FbaI 1599 TGATCA T^GATCA
4554 TGATCA
HaeII 2024 GGCGCT [GA]GCGC^[CT]
2729 GGCGCT
HhaI 2025 GCGC GCG^C
2730 GCGC
HinP1I 2025 GCGC G^CGC
2730 GCGC
HindIII 3443 AAGCTT A^AGCTT
3552 AAGCTT
HpaI 18 GTTAAC GTT^AAC
4523 GTTAAC
PvuII 3425 CAGCTG CAG^CTG
3895 CAGCTG
SapI 2617 GAAGAGC GCTCTTC(1/4)
852 GCTCTTC
SspI 3758 AATATT AAT^ATT
4472 AATATT
StuI 3209 AGGCCT AGG^CCT
3720 AGGCCT
StyI 1694 CCTTGG C^C[AT][AT]GG
2906 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BamHI BanII
BbsI BbvI BciVI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspLU11I
BspMI BsrGI BsrI BssKI BstEII
BstF5I BstNI BstPI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoO65I EcoRI EcoRII
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PshBI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI