##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AviII BamHI BbrPI
BcgI BfrI BsaWI BseAI BsiWI
BsmBI BsmI BspEI BspHI BssHII
BstBI CpoI Eam1105I Eco47III EcoNI
EcoRV FseI FspI HpaI KpnI
MfeI MluI MroI MunI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SphI Sse8387I
StuI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 443 GACGTC GACGT^C
AflIII 995 ACATGT A^C[GA][CT]GT
AlwNI 2249 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 3754 ATTAAT AT^TAAT
AsnI 3754 ATTAAT AT^TAAT
AspI 1105 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3292 CCTAGG C^CTAGG
BclI 3438 TGATCA T^GATCA
BlnI 3292 CCTAGG C^CTAGG
BsaAI 4865 TACGTA [CT]AC^GT[GA]
BsaBI 3778 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 34 GTGCAG GTGCAG(16/14)
BsiEI 131 CGGCCG CG[GA][CT]^CG
Bsp1407I 1384 TGTACA T^GTACA
BspDI 901 ATCGAT AT^CGAT
BspLU11I 995 ACATGT A^CATGT
BsrFI 3 GCCGGT [GA]^CCGG[CT]
BsrGI 1384 TGTACA T^GTACA
BssSI 2189 CACGAG CACGAG(-5/-1)
Bst1107I 3599 GTATAC GTA^TAC
BstAPI 2122 GCATCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1404 GGTAACC G^GT[ACGT]ACC
BstPI 1404 GGTAACC G^GT[ACGT]ACC
BstZ17I 3599 GTATAC GTA^TAC
Cfr10I 3 GCCGGT [GA]^CCGG[CT]
ClaI 901 ATCGAT AT^CGAT
DraIII 1976 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 352 GACGGCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 131 CGGCCG C^GGCCG
EclXI 131 CGGCCG C^GGCCG
Eco52I 131 CGGCCG C^GGCCG
EcoO65I 1404 GGTAACC G^GT[ACGT]ACC
FbaI 3438 TGATCA T^GATCA
HgaI 274 GCGTC GACGC(5/10)
HincII 1534 GTTGAC GT[CT]^[GA]AC
HindII 1534 GTTGAC GT[CT]^[GA]AC
MamI 3778 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 995 ACATGT [GA]CATG^[CT]
PflFI 1105 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3754 ATTAAT AT^TAAT
PstI 3043 CTGCAG CTGCA^G
PvuII 2428 CAGCTG CAG^CTG
SacI 674 GAGCTC GAGCT^C
SnaBI 4865 TACGTA TAC^GTA
SpeI 2982 ACTAGT A^CTAGT
SspBI 1384 TGTACA T^GTACA
Tth111I 1105 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1708 GTCTAC GT^[AC][GT]AC
3599 GTATAC
Asp700I 1150 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1928 GAAAGAATTC
BbeI 142 GGCGCC GGCGC^C
369 GGCGCC
BbsI 3502 GTCTTC GAAGAC(2/6)
4189 GTCTTC
BlpI 494 GCTCAGC GC^T[ACGT]AGC
3607 GCTCAGC
Bpu1102I 494 GCTCAGC GC^T[ACGT]AGC
3607 GCTCAGC
BpuAI 3502 GTCTTC GAAGAC(2/6)
4189 GTCTTC
BseRI 4569 CTCCTC GAGGAG(10/8)
1024 GAGGAG
BsrBI 239 CCGCTC CCGCTC(-3/-3)
199 GAGCGG
CelII 494 GCTCAGC GC^T[ACGT]AGC
3607 GCTCAGC
DraI 4524 TTTAAA TTT^AAA
4717 TTTAAA
EcoRI 415 GAATTC G^AATTC
1932 GAATTC
EcoT22I 4218 ATGCAT ATGCA^T
4734 ATGCAT
HaeII 142 GGCGCC [GA]GCGC^[CT]
369 GGCGCC
HindIII 4632 AAGCTT A^AGCTT
4749 AAGCTT
KasI 142 GGCGCC G^GCGCC
369 GGCGCC
KspI 50 CCGCGG CCGC^GG
194 CCGCGG
MslI 3019 CATTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4453 CACAGGTGTG
NarI 142 GGCGCC GG^CGCC
369 GGCGCC
NdeI 3639 CATATG CA^TATG
4594 CATATG
NsiI 4218 ATGCAT ATGCA^T
4734 ATGCAT
PflMI 2037 CCAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3202 CCAAAAATTGG
PpuMI 86 AGGACCT [GA]G^G[AT]CC[CT]
2635 GGGACCC
SacII 50 CCGCGG CCGC^GG
194 CCGCGG
ScaI 1569 AGTACT AGT^ACT
3653 AGTACT
SfoI 142 GGCGCC GGC^GCC
369 GGCGCC
SmaI 219 CCCGGG CCC^GGG
2279 CCCGGG
TaqI 902 TCGA T^CGA
1332 TCGA
TthHB8I 902 TCGA T^CGA
1332 TCGA
Van91I 2037 CCAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3202 CCAAAAATTGG
XmaCI 219 CCCGGG C^CCGGG
2279 CCCGGG
XmaI 219 CCCGGG C^CCGGG
2279 CCCGGG
XmnI 1150 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1928 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BciVI BcnI
BfaI BglI BglII BmyI BpmI
BsaHI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII