##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI AscI
Asp718I AspEI AspI AvrII BbrPI
BbvCI BcgI BglI BlnI BlpI
Bpu1102I BsaWI BseAI BsiEI BsiWI
BsmI BspEI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstZ17I
CelII Cfr10I CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
FseI KpnI KspI MluI MroI
NaeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfuI SgrAI Sse8387I SwaI
Tth111I XcmI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 746 GACGTC GACGT^C
AocI 1198 CCTAAGG CC^T[ACGT]AGG
ApaI 1739 GGGCCC GGGCC^C
AseI 3456 ATTAAT AT^TAAT
AsnI 3456 ATTAAT AT^TAAT
BamHI 2533 GGATCC G^GATCC
BbeI 186 GGCGCC GGCGC^C
BsaBI 1327 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1925 GAGACC GGTCTC(1/5)
BspDI 1822 ATCGAT AT^CGAT
BstEII 2249 GGTCACC G^GT[ACGT]ACC
BstPI 2249 GGTCACC G^GT[ACGT]ACC
Bsu36I 1198 CCTAAGG CC^T[ACGT]AGG
ClaI 1822 ATCGAT AT^CGAT
Eco81I 1198 CCTAAGG CC^T[ACGT]AGG
EcoNI 5170 CCTGATTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2249 GGTCACC G^GT[ACGT]ACC
HapII 142 CCGG C^CGG
HpaII 142 CCGG C^CGG
KasI 186 GGCGCC G^GCGCC
MamI 1327 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 596 CAATTG C^AATTG
MspI 142 CCGG C^CGG
MunI 596 CAATTG C^AATTG
NarI 186 GGCGCC GG^CGCC
NcoI 4044 CCATGG C^CATGG
PaeR7I 2759 CTCGAG C^TCGAG
PflMI 2616 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3619 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3456 ATTAAT AT^TAAT
SapI 1651 GCTCTTC GCTCTTC(1/4)
SexAI 2209 ACCAGGT A^CC[AT]GGT
SfoI 186 GGCGCC GGC^GCC
SmaI 141 CCCGGG CCC^GGG
SnaBI 1843 TACGTA TAC^GTA
StuI 2272 AGGCCT AGG^CCT
Van91I 2616 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2759 CTCGAG C^TCGAG
XmaCI 141 CCCGGG C^CCGGG
XmaI 141 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 2889 CTTAAG C^TTAAG
3247 CTTAAG
AvaI 141 CCCGGG C^[CT]CG[GA]G
2759 CTCGAG
AviII 2666 TGCGCA TGC^GCA
4610 TGCGCA
BalI 936 TGGCCA TGG^CCA
2471 TGGCCA
BanI 186 GGCGCC G^G[CT][GA]CC
5632 GGTGCC
BclI 1332 TGATCA T^GATCA
2082 TGATCA
BcnI 141 CCCGG CC^[GC]GG
142 CCGGG
BfrI 2889 CTTAAG C^TTAAG
3247 CTTAAG
BglII 975 AGATCT A^GATCT
2037 AGATCT
BsaAI 1843 TACGTA [CT]AC^GT[GA]
3785 TACGTG
BsoBI 141 CCCGGG C^[CT]CG[GA]G
2759 CTCGAG
BspMI 5484 ACCTGC ACCTGC(4/8)
5651 GCAGGT
BstXI 365 CCAATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
452 CCATCAAGTTGG
DsaI 3241 CCACGG C^C[GA][CT]GG
4044 CCATGG
EaeI 936 TGGCCA [CT]^GGCC[GA]
2471 TGGCCA
EcoRI 1447 GAATTC G^AATTC
3928 GAATTC
EcoRV 3261 GATATC GAT^ATC
3640 GATATC
FbaI 1332 TGATCA T^GATCA
2082 TGATCA
FspI 2666 TGCGCA TGC^GCA
4610 TGCGCA
HgaI 202 GCGTC GACGC(5/10)
208 GCGTC
MluNI 936 TGGCCA TGG^CCA
2471 TGGCCA
MscI 936 TGGCCA TGG^CCA
2471 TGGCCA
NciI 141 CCCGG CC^[GC]GG
142 CCGGG
PleI 1626 GAGTC GAGTC(4/5)
3912 GAGTC
ScaI 1157 AGTACT AGT^ACT
3750 AGTACT
SphI 2669 GCATGC GCATG^C
2764 GCATGC
SspI 1234 AATATT AAT^ATT
4890 AATATT
XbaI 2418 TCTAGA T^CTAGA
3615 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI ApoI
Asp700I AspHI AvaII BanII BbsI
BbvI BciVI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I Bsp1407I BspLU11I BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MvnI MwoI NdeI
NdeII NlaIII NlaIV NsiI NspI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SpeI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI