HUGE

Gene/Protein Characteristic Table for KIAA0830

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00135
Accession No. :	AB020637
Description :	Endonuclease domain-containing 1 protein precursor.
HUGO Gene Name :	endonuclease domain containing 1 (ENDOD1)
Clone Name :	hj04436 [Vector Info]
Flexi ORF Clone :	pF1KA0830
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 4638 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 3066 bp

Genome contig ID gi51511727f_94362671
PolyA signal sequence
(AATAAA,-18)
+----*----+----*----+----*----+----
GCACCTTGCAGATGCTCAATAAAGTAATTACTGAC
Flanking genome sequence
(142786 - 142835)
----+----*----+----*----+----*----+----*----+----*
AACTCGTTAGCAGTTCTGTAAAATATCTAAAAGGGGAGAATCCTAATAGT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 11 f 94462671 94505455 2 99.1 Perfect prediction

Features of the protein sequence	Description

Length: 523 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
O94919	1.4e-208	100.0	Endonuclease do...	Homo sapiens
BAF84362	1.5e-207	99.6	unnamed protein...	Homo sapiens
XP_522156	3.8e-207	99.4	endonuclease do...	Pan troglodytes
XP_001090534	1.4e-203	97.0	endonuclease do...	Macaca mulatta
AAI51614	3.9e-164	76.6	ENDOD1 protein ...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR001604 84 289 PF01223 DNA/RNA non-specific endonuclease

HMMSmart IPR001604 85 290 SM00477 DNA/RNA non-specific endonuclease

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001604	84	289	PF01223	DNA/RNA non-specific endonuclease
HMMSmart	IPR001604	85	290	SM00477	DNA/RNA non-specific endonuclease

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 23 AMGTARWLALGSLFALAGLLEGR 45 SECONDARY 23

2 235 KVAVPEFVWLAACCAVPGGGWAM 257 PRIMARY 23

3 373 VVAILKNIVYFLWCVTKQVINGI 395 PRIMARY 23

4 424 VLKVVAKVIRALLRILCCLLKAI 446 PRIMARY 23

5 479 ALGLGGTVSLLFDTAFGTLGGLF 501 SECONDARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	23	AMGTARWLALGSLFALAGLLEGR	45	SECONDARY	23
2	235	KVAVPEFVWLAACCAVPGGGWAM	257	PRIMARY	23
3	373	VVAILKNIVYFLWCVTKQVINGI	395	PRIMARY	23
4	424	VLKVVAKVIRALLRILCCLLKAI	446	PRIMARY	23
5	479	ALGLGGTVSLLFDTAFGTLGGLF	501	SECONDARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: CCTGTTCCTAATGAGCTACGC

Primer_r
: GGTTCACTTTGGTTCCTCCTC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 11

Experimental conditions

Panel name	: UniGene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	3066 bp
Genome contig ID	gi51511727f_94362671
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- GCACCTTGCAGATGCTCAATAAAGTAATTACTGAC
Flanking genome sequence (142786 - 142835)	----+--------+--------+--------+--------+----* AACTCGTTAGCAGTTCTGTAAAATATCTAAAAGGGGAGAATCCTAATAGT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	11	f	94462671	94505455	2	99.1	Perfect prediction

Primer_f	: CCTGTTCCTAATGAGCTACGC
Primer_r	: GGTTCACTTTGGTTCCTCCTC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA0830

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information