##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AflII AgeI AhdI AocI
ApaI AscI Asp700I AspEI AviII
AvrII BfrI BlnI BsaBI BsgI
BsiWI BsmBI BspHI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CpoI
DrdI Eam1105I Eco81I EcoO65I EcoRI
EcoRV FseI FspI HgaI MamI
MluI NdeI NotI NruI NspV
PacI PaeR7I PinAI PmeI PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SphI Sse8387I SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 1483 GGTACC G^GTACC
AccI 1005 GTCTAC GT^[AC][GT]AC
AccIII 1307 TCCGGA T^CCGGA
AclI 3495 AACGTT AA^CGTT
AcyI 337 GGCGCC G[GA]^CG[CT]C
Aor51HI 257 AGCGCT AGC^GCT
Asp718I 1483 GGTACC G^GTACC
AspI 567 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1310 GGATCC G^GATCC
BbeI 337 GGCGCC GGCGC^C
BbrPI 223 CACGTG CAC^GTG
BbvCI 853 CCTCAGC CCTCAGC(-5/-2)
BsaHI 337 GGCGCC G[GA]^CG[CT]C
BseAI 1307 TCCGGA T^CCGGA
BsiEI 212 CGGCCG CG[GA][CT]^CG
BspDI 370 ATCGAT AT^CGAT
BspEI 1307 TCCGGA T^CCGGA
BsrBI 37 CCGCTC CCGCTC(-3/-3)
BssSI 136 CTCGTG CACGAG(-5/-1)
ClaI 370 ATCGAT AT^CGAT
DraIII 632 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 212 CGGCCG C^GGCCG
EclXI 212 CGGCCG C^GGCCG
Eco47III 257 AGCGCT AGC^GCT
Eco52I 212 CGGCCG C^GGCCG
Hin1I 337 GGCGCC G[GA]^CG[CT]C
HindIII 1090 AAGCTT A^AGCTT
HpaI 925 GTTAAC GTT^AAC
KasI 337 GGCGCC G^GCGCC
KpnI 1483 GGTACC GGTAC^C
KspI 23 CCGCGG CCGC^GG
MroI 1307 TCCGGA T^CCGGA
NarI 337 GGCGCC GG^CGCC
PflFI 567 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 631 CCACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 223 CACGTG CAC^GTG
PmlI 223 CACGTG CAC^GTG
PshAI 2584 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3495 AACGTT AA^CGTT
PvuII 856 CAGCTG CAG^CTG
SacII 23 CCGCGG CCGC^GG
SapI 3120 GAAGAGC GCTCTTC(1/4)
SfoI 337 GGCGCC GGC^GCC
SpeI 3959 ACTAGT A^CTAGT
SspI 2684 AATATT AAT^ATT
StuI 4136 AGGCCT AGG^CCT
Tth111I 567 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 631 CCACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3984 TCTAGA T^CTAGA
XcmI 2867 CCACAATTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Alw44I 3208 GTGCAC G^TGCAC
4602 GTGCAC
ApaLI 3208 GTGCAC G^TGCAC
4602 GTGCAC
AvaI 281 CCCGGG C^[CT]CG[GA]G
788 CCCGGG
BbsI 649 GAAGAC GAAGAC(2/6)
4240 GTCTTC
BcgI 4265 CGATCTAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
98 GCAGCCTCTTCG
BciVI 1589 GTATCC GTATCC(6/5)
2458 GTATCC
BclI 2799 TGATCA T^GATCA
3146 TGATCA
BglI 198 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1442 GCCTTGGTGGC
BpuAI 649 GAAGAC GAAGAC(2/6)
4240 GTCTTC
BsaAI 223 CACGTG [CT]AC^GT[GA]
4350 CACGTA
BsaI 3939 GAGACC GGTCTC(1/5)
3980 GGTCTC
BsaWI 1307 TCCGGA [AT]^CCGG[AT]
2360 TCCGGT
BsmI 2073 GAATGC GAATGC(1/-1)
1256 GCATTC
BsoBI 281 CCCGGG C^[CT]CG[GA]G
788 CCCGGG
BspLU11I 3507 ACATGT A^CATGT
4490 ACATGT
BspMI 1167 GCAGGT ACCTGC(4/8)
4017 GCAGGT
BsrFI 131 GCCGGC [GA]^CCGG[CT]
154 GCCGGC
BssHII 79 GCGCGC G^CGCGC
240 GCGCGC
BstAPI 2077 GCAGATAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2259 GCATTTTATGC
Cfr10I 131 GCCGGC [GA]^CCGG[CT]
154 GCCGGC
EcoT22I 2546 ATGCAT ATGCA^T
4385 ATGCAT
FbaI 2799 TGATCA T^GATCA
3146 TGATCA
HincII 925 GTTAAC GT[CT]^[GA]AC
3029 GTCAAC
HindII 925 GTTAAC GT[CT]^[GA]AC
3029 GTCAAC
MspA1I 23 CCGCGG C[AC]G^C[GT]G
856 CAGCTG
NaeI 131 GCCGGC GCC^GGC
154 GCCGGC
NgoMIV 131 GCCGGC G^CCGGC
154 GCCGGC
NheI 3451 GCTAGC G^CTAGC
3470 GCTAGC
NsiI 2546 ATGCAT ATGCA^T
4385 ATGCAT
PleI 567 GACTC GAGTC(4/5)
3605 GACTC
PpuMI 190 GGGACCC [GA]G^G[AT]CC[CT]
3003 GGGTCCC
SacI 14 GAGCTC GAGCT^C
2777 GAGCTC
SmaI 281 CCCGGG CCC^GGG
788 CCCGGG
XmaCI 281 CCCGGG C^CCGGG
788 CCCGGG
XmaI 281 CCCGGG C^CCGGG
788 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaII BalI BanI
BanII BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspI MunI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII