##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccII AccIII AflIII AhdI
ApaI AscI AspEI AvaI AviII
BamHI BbeI BbrPI BcgI BciVI
BclI BcnI BseAI BseRI BsiWI
BsoBI BspDI BspEI BspLU11I BsrBI
BssHII BssSI BstBI BstEII BstPI
BstUI ClaI CpoI DraII EagI
Eam1105I EclXI Eco52I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI KasI KspI MluI
MroI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PpuMI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 564 GGTACC G^GTACC
AccI 1094 GTATAC GT^[AC][GT]AC
AcyI 341 GACGCC G[GA]^CG[CT]C
AflII 2289 CTTAAG C^TTAAG
AgeI 1098 ACCGGT A^CCGGT
AlwNI 3238 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1345 AGCGCT AGC^GCT
Asp718I 564 GGTACC G^GTACC
AspI 305 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4844 CCTAGG C^CTAGG
BanI 564 GGTACC G^G[CT][GA]CC
BanII 2728 GAGCTC G[GA]GC[CT]^C
BbvCI 824 CCTCAGC CCTCAGC(-5/-2)
BfrI 2289 CTTAAG C^TTAAG
BglII 897 AGATCT A^GATCT
BlnI 4844 CCTAGG C^CTAGG
BlpI 3120 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3120 GCTAAGC GC^T[ACGT]AGC
BsaAI 762 TACGTG [CT]AC^GT[GA]
BsaHI 341 GACGCC G[GA]^CG[CT]C
BsmBI 4828 CGTCTC CGTCTC(1/5)
BspHI 708 TCATGA T^CATGA
BspMI 4839 ACCTGC ACCTGC(4/8)
BsrDI 2320 CATTGC GCAATG(2/0)
Bst1107I 1094 GTATAC GTA^TAC
BstAPI 2302 GCATCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 319 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1094 GTATAC GTA^TAC
CelII 3120 GCTAAGC GC^T[ACGT]AGC
DraIII 954 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 914 GACCCTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2895 CCATGG C^C[GA][CT]GG
Eco47III 1345 AGCGCT AGC^GCT
HaeII 1345 AGCGCT [GA]GCGC^[CT]
Hin1I 341 GACGCC G[GA]^CG[CT]C
HpaI 1617 GTTAAC GTT^AAC
KpnI 564 GGTACC GGTAC^C
NcoI 2895 CCATGG C^CATGG
PflFI 305 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1098 ACCGGT A^CCGGT
RcaI 708 TCATGA T^CATGA
SacI 2728 GAGCTC GAGCT^C
ScaI 4306 AGTACT AGT^ACT
SphI 729 GCATGC GCATG^C
StuI 1174 AGGCCT AGG^CCT
SwaI 4730 ATTTAAAT ATTT^AAAT
Tth111I 305 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AclI 2205 AACGTT AA^CGTT
2296 AACGTT
Alw44I 127 GTGCAC G^TGCAC
2847 GTGCAC
AocI 1423 CCTGAGG CC^T[ACGT]AGG
3540 CCTCAGG
ApaLI 127 GTGCAC G^TGCAC
2847 GTGCAC
AseI 2615 ATTAAT AT^TAAT
3096 ATTAAT
AsnI 2615 ATTAAT AT^TAAT
3096 ATTAAT
BalI 373 TGGCCA TGG^CCA
1366 TGGCCA
BglI 324 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
593 GCCTGCAAGGC
BsaI 1101 GGTCTC GGTCTC(1/5)
2080 GGTCTC
BsaWI 889 ACCGGA [AT]^CCGG[AT]
1098 ACCGGT
BsiEI 146 CGGTCG CG[GA][CT]^CG
970 CGACCG
BsmI 602 GCATTC GAATGC(1/-1)
3440 GCATTC
Bsp1407I 3339 TGTACA T^GTACA
3531 TGTACA
BsrFI 144 GCCGGT [GA]^CCGG[CT]
1098 ACCGGT
BsrGI 3339 TGTACA T^GTACA
3531 TGTACA
Bsu36I 1423 CCTGAGG CC^T[ACGT]AGG
3540 CCTCAGG
Cfr10I 144 GCCGGT [GA]^CCGG[CT]
1098 ACCGGT
Eco81I 1423 CCTGAGG CC^T[ACGT]AGG
3540 CCTCAGG
EcoNI 5 CCTTGATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2111 CCTGCCAAAGG
HincII 311 GTCAAC GT[CT]^[GA]AC
1617 GTTAAC
HindII 311 GTCAAC GT[CT]^[GA]AC
1617 GTTAAC
MfeI 2871 CAATTG C^AATTG
5014 CAATTG
MluNI 373 TGGCCA TGG^CCA
1366 TGGCCA
MscI 373 TGGCCA TGG^CCA
1366 TGGCCA
MslI 4048 CATTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4729 CATTTAAATG
MunI 2871 CAATTG C^AATTG
5014 CAATTG
NdeI 2878 CATATG CA^TATG
4599 CATATG
NspI 507 ACATGC [GA]CATG^[CT]
729 GCATGC
PshAI 1382 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3449 GACTGTTGTC
PshBI 2615 ATTAAT AT^TAAT
3096 ATTAAT
Psp1406I 2205 AACGTT AA^CGTT
2296 AACGTT
SapI 206 GAAGAGC GCTCTTC(1/4)
333 GCTCTTC
SspBI 3339 TGTACA T^GTACA
3531 TGTACA
XbaI 2333 TCTAGA T^CTAGA
4023 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaBI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI