##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI Asp700I Asp718I AviII BamHI
BbeI BbvCI BciVI BspDI BspLU11I
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI Eco47III EcoRV FseI FspI
HindIII HpaI KasI KpnI MfeI
MluI MunI NarI NdeI NheI
NotI NspV PacI PaeR7I PinAI
Psp1406I PvuI RsrII SalI SfoI
SfuI SnaBI SpeI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 4197 GTCTAC GT^[AC][GT]AC
AccIII 4811 TCCGGA T^CCGGA
AflII 6068 CTTAAG C^TTAAG
Alw44I 736 GTGCAC G^TGCAC
AocI 4472 CCTCAGG CC^T[ACGT]AGG
ApaI 2639 GGGCCC GGGCC^C
ApaLI 736 GTGCAC G^TGCAC
AseI 6296 ATTAAT AT^TAAT
AsnI 6296 ATTAAT AT^TAAT
BbrPI 2188 CACGTG CAC^GTG
BcgI 368 CGAAAAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 6068 CTTAAG C^TTAAG
BsaBI 3135 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4811 TCCGGA T^CCGGA
BsiWI 1697 CGTACG C^GTACG
BsmBI 2194 GAGACG CGTCTC(1/5)
BspEI 4811 TCCGGA T^CCGGA
BssSI 1144 CACGAG CACGAG(-5/-1)
Bsu36I 4472 CCTCAGG CC^T[ACGT]AGG
DraIII 3269 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2765 CGGCCG C^GGCCG
EclXI 2765 CGGCCG C^GGCCG
Eco52I 2765 CGGCCG C^GGCCG
Eco81I 4472 CCTCAGG CC^T[ACGT]AGG
EcoNI 1788 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 5911 ATGCAT ATGCA^T
HincII 3508 GTTGAC GT[CT]^[GA]AC
HindII 3508 GTTGAC GT[CT]^[GA]AC
MamI 3135 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4811 TCCGGA T^CCGGA
NruI 191 TCGCGA TCG^CGA
NsiI 5911 ATGCAT ATGCA^T
PmaCI 2188 CACGTG CAC^GTG
PmeI 5944 GTTTAAAC GTTT^AAAC
PmlI 2188 CACGTG CAC^GTG
PshAI 2992 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6296 ATTAAT AT^TAAT
SbfI 2058 CCTGCAGG CCTGCA^GG
SexAI 1247 ACCAGGT A^CC[AT]GGT
SfiI 2766 GGCCGCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2394 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2792 GCATGC GCATG^C
Sse8387I 2058 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AhdI 1987 GACTACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3103 GACTGGCTGTC
AspEI 1987 GACTACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3103 GACTGGCTGTC
AspI 724 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3186 GACGGGGTC
AvrII 2787 CCTAGG C^CTAGG
3564 CCTAGG
BbsI 3872 GAAGAC GAAGAC(2/6)
5228 GAAGAC
BglII 2930 AGATCT A^GATCT
4179 AGATCT
BlnI 2787 CCTAGG C^CTAGG
3564 CCTAGG
BpuAI 3872 GAAGAC GAAGAC(2/6)
5228 GAAGAC
BsaAI 2188 CACGTG [CT]AC^GT[GA]
5342 CACGTA
BsiEI 188 CGGTCG CG[GA][CT]^CG
2765 CGGCCG
BsmI 4360 GAATGC GAATGC(1/-1)
5662 GAATGC
BspHI 4761 TCATGA T^CATGA
6133 TCATGA
DraI 5945 TTTAAA TTT^AAA
6535 TTTAAA
Eam1105I 1987 GACTACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3103 GACTGGCTGTC
EcoRI 4170 GAATTC G^AATTC
4696 GAATTC
PflFI 724 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3186 GACGGGGTC
RcaI 4761 TCATGA T^CATGA
6133 TCATGA
StuI 1710 AGGCCT AGG^CCT
2156 AGGCCT
Tth111I 724 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3186 GACGGGGTC
XcmI 1241 CCACCAACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1937 CCAGCACCACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvI BclI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmaI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI