##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
AscI Asp718I AspEI AvaI AvrII
BamHI BanI BbeI BcgI BcnI
BfrI BlnI BsaHI BsaWI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BspHI BsrBI BsrFI BssHII BssSI
BstBI BstUI Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV EcoT22I FauI FseI
HaeII HapII HgaI Hin1I HpaII
KasI KpnI KspI MluI MroI
MspA1I MspI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I StuI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 2992 GTATAC GT^[AC][GT]AC
Alw44I 4647 GTGCAC G^TGCAC
ApaI 1001 GGGCCC GGGCC^C
ApaLI 4647 GTGCAC G^TGCAC
AseI 2339 ATTAAT AT^TAAT
AsnI 2339 ATTAAT AT^TAAT
Asp700I 350 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 26 TGCGCA TGC^GCA
BbrPI 176 CACGTG CAC^GTG
BbvCI 3931 CCTCAGC CCTCAGC(-5/-2)
BciVI 821 GTATCC GTATCC(6/5)
BclI 2861 TGATCA T^GATCA
BglI 1175 GCCCACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 956 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 956 GCTCAGC GC^T[ACGT]AGC
BsaAI 176 CACGTG [CT]AC^GT[GA]
BseRI 666 CTCCTC GAGGAG(10/8)
BsmBI 334 GAGACG CGTCTC(1/5)
Bst1107I 2992 GTATAC GTA^TAC
BstZ17I 2992 GTATAC GTA^TAC
CelII 956 GCTCAGC GC^T[ACGT]AGC
DraIII 4641 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 4591 CCTTCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2861 TGATCA T^GATCA
FspI 26 TGCGCA TGC^GCA
HindIII 3393 AAGCTT A^AGCTT
HpaI 1354 GTTAAC GTT^AAC
MfeI 3406 CAATTG C^AATTG
MunI 3406 CAATTG C^AATTG
NdeI 4554 CATATG CA^TATG
PmaCI 176 CACGTG CAC^GTG
PmlI 176 CACGTG CAC^GTG
PshAI 1658 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2339 ATTAAT AT^TAAT
PstI 1551 CTGCAG CTGCA^G
SapI 928 GCTCTTC GCTCTTC(1/4)
ScaI 49 AGTACT AGT^ACT
SexAI 1035 ACCTGGT A^CC[AT]GGT
SpeI 2879 ACTAGT A^CTAGT
XmnI 350 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AocI 209 CCTCAGG CC^T[ACGT]AGG
1048 CCTCAGG
BglII 2978 AGATCT A^GATCT
3354 AGATCT
BpmI 3579 CTCCAG CTGGAG(16/14)
4437 CTCCAG
BsaBI 16 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3349 GATACAGATC
BsaI 908 GAGACC GGTCTC(1/5)
3531 GGTCTC
BspLU11I 4537 ACATGT A^CATGT
4651 ACATGT
BspMI 3246 ACCTGC ACCTGC(4/8)
4038 GCAGGT
BstEII 1252 GGTGACC G^GT[ACGT]ACC
3242 GGTTACC
BstPI 1252 GGTGACC G^GT[ACGT]ACC
3242 GGTTACC
Bsu36I 209 CCTCAGG CC^T[ACGT]AGG
1048 CCTCAGG
CfoI 27 GCGC GCG^C
4398 GCGC
Eco81I 209 CCTCAGG CC^T[ACGT]AGG
1048 CCTCAGG
EcoO65I 1252 GGTGACC G^GT[ACGT]ACC
3242 GGTTACC
EcoRI 434 GAATTC G^AATTC
3272 GAATTC
HhaI 27 GCGC GCG^C
4398 GCGC
HinP1I 27 GCGC G^CGC
4398 GCGC
HincII 1354 GTTAAC GT[CT]^[GA]AC
2496 GTTGAC
HindII 1354 GTTAAC GT[CT]^[GA]AC
2496 GTTGAC
MamI 16 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3349 GATACAGATC
NcoI 1308 CCATGG C^CATGG
1996 CCATGG
NspI 4537 ACATGT [GA]CATG^[CT]
4651 ACATGT
PflMI 1996 CCATGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3037 CCATACAATGG
SacI 1831 GAGCTC GAGCT^C
2195 GAGCTC
Van91I 1996 CCATGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3037 CCATACAATGG
XcmI 1170 CCACAGCCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4530 CCATTGTACATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AspI
AvaII BalI BanII BbsI BbvI
BfaI BmyI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV PflFI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XbaI
XhoII