HUGE

Gene/Protein Characteristic Table for KIAA0837

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK01127
Accession No. :	AB020644
Description :	Long-chain-fatty-acid--CoA ligase 6.
HUGO Gene Name :	acyl-CoA synthetase long-chain family member 6 (ACSL6)
Clone Name :	hj06536 [Vector Info]
Flexi ORF Clone :	pF1KA0837
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 4868 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 2630 bp

Genome contig ID gi51511721r_131215479
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
ACTCCAGCCTGGGCAACAGAACGAGACTCCGTCTC
Flanking genome sequence
(99717 - 99668)
----+----*----+----*----+----*----+----*----+----*
AAAAAGAAAGAAAAAAAAAAAACAAAAACAAGGTTTCTTCTTTTTGCCAC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 5 r 131315196 131375214 21 99.4 Perfect prediction

Features of the protein sequence	Description

Length: 745 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAG11245	100.0	acyl-CoA synthe...	synthetic construct
NP_056071	98.5	acyl-CoA synthe...	Homo sapiens
Q9UKU0	100.0	Long-chain-fatt...	Homo sapiens
BAE02116	99.1	unnamed protein...	Macaca fascicularis
EAW62358	98.4	acyl-CoA synthe...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB014531	1.5e-30	26.3	KIAA0631

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR000873 316 327 PR00154 AMP-dependent synthetase and ligase

IPR000873 328 336 PR00154 AMP-dependent synthetase and ligase

HMMPfam IPR000873 170 438 PF00501 AMP-dependent synthetase and ligase

IPR000873 476 636 PF00501 AMP-dependent synthetase and ligase

ScanRegExp IPR000873 321 332 PS00455 AMP-dependent synthetase and ligase

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000873	316	327	PR00154	AMP-dependent synthetase and ligase
IPR000873	328	336	PR00154	AMP-dependent synthetase and ligase
HMMPfam	IPR000873	170	438	PF00501	AMP-dependent synthetase and ligase
IPR000873	476	636	PF00501	AMP-dependent synthetase and ligase
ScanRegExp	IPR000873	321	332	PS00455	AMP-dependent synthetase and ligase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 22 TAMLTFFLVSGGSLWLFVEFVLS 44 PRIMARY 23

2 73 LSATTLVSMGALAAILAYWFTHR 95 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	22	TAMLTFFLVSGGSLWLFVEFVLS	44	PRIMARY	23
2	73	LSATTLVSMGALAAILAYWFTHR	95	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TTTCCCATTGTCCTCCTACTC

Primer_r
: TCTCCTGACCTCGTGATCTGC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 5

Experimental conditions

Panel name	: UniGene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	2630 bp
Genome contig ID	gi51511721r_131215479
PolyA signal sequence (None)	+--------+--------+----*----+---- ACTCCAGCCTGGGCAACAGAACGAGACTCCGTCTC
Flanking genome sequence (99717 - 99668)	----+--------+--------+--------+--------+----* AAAAAGAAAGAAAAAAAAAAAACAAAAACAAGGTTTCTTCTTTTTGCCAC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	5	r	131315196	131375214	21	99.4	Perfect prediction

Primer_f	: TTTCCCATTGTCCTCCTACTC
Primer_r	: TCTCCTGACCTCGTGATCTGC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA0837

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information