##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AscI Asp718I AspEI AviII
BbrPI BcgI BfrI BsaBI BseRI
BsiEI BsiWI BspDI BssHII Bst1107I
BstAPI BstEII BstPI BstZ17I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I FseI FspI
HgaI HindIII HpaI KpnI KspI
MamI MluI NheI NotI NruI
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
RsrII SacII SalI SbfI SfiI
SgrAI SmaI SpeI Sse8387I SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 2742 GTCTAC GT^[AC][GT]AC
AccII 4593 CGCG CG^CG
Alw44I 1530 GTGCAC G^TGCAC
Aor51HI 1301 AGCGCT AGC^GCT
ApaLI 1530 GTGCAC G^TGCAC
AseI 3410 ATTAAT AT^TAAT
AsnI 3410 ATTAAT AT^TAAT
AvrII 2890 CCTAGG C^CTAGG
BciVI 167 GGATAC GTATCC(6/5)
BlnI 2890 CCTAGG C^CTAGG
BlpI 441 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 441 GCTTAGC GC^T[ACGT]AGC
BsmI 3985 GAATGC GAATGC(1/-1)
Bsp1407I 4288 TGTACA T^GTACA
BspHI 2823 TCATGA T^CATGA
BspMI 2097 GCAGGT ACCTGC(4/8)
BsrBI 670 CCGCTC CCGCTC(-3/-3)
BsrGI 4288 TGTACA T^GTACA
BstBI 832 TTCGAA TT^CGAA
BstUI 4593 CGCG CG^CG
BstXI 656 CCATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 441 GCTTAGC GC^T[ACGT]AGC
Eco47III 1301 AGCGCT AGC^GCT
EcoNI 181 CCTGAGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3315 GAATTC G^AATTC
EcoRV 1183 GATATC GAT^ATC
EcoT22I 4059 ATGCAT ATGCA^T
MfeI 564 CAATTG C^AATTG
MunI 564 CAATTG C^AATTG
MvnI 4593 CGCG CG^CG
NaeI 1779 GCCGGC GCC^GGC
NgoMIV 1779 GCCGGC G^CCGGC
NsiI 4059 ATGCAT ATGCA^T
NspV 832 TTCGAA TT^CGAA
PaeR7I 2946 CTCGAG C^TCGAG
PshBI 3410 ATTAAT AT^TAAT
RcaI 2823 TCATGA T^CATGA
SacI 3069 GAGCTC GAGCT^C
SapI 2212 GAAGAGC GCTCTTC(1/4)
ScaI 2192 AGTACT AGT^ACT
SexAI 422 ACCAGGT A^CC[AT]GGT
SfuI 832 TTCGAA TT^CGAA
SnaBI 2745 TACGTA TAC^GTA
SspBI 4288 TGTACA T^GTACA
XbaI 3961 TCTAGA T^CTAGA
XhoI 2946 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 1587 GGCGCC G[GA]^CG[CT]C
4739 GGCGCC
AlwNI 499 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2648 CAGTAACTG
AocI 162 CCTGAGG CC^T[ACGT]AGG
1571 CCTCAGG
Asp700I 2793 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3503 GAACAATTTC
AspI 2871 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4858 GACTCCGTC
BalI 906 TGGCCA TGG^CCA
1518 TGGCCA
BamHI 3078 GGATCC G^GATCC
3351 GGATCC
BbeI 1587 GGCGCC GGCGC^C
4739 GGCGCC
BbvCI 379 CCTCAGC CCTCAGC(-5/-2)
4767 GCTGAGG
BclI 582 TGATCA T^GATCA
812 TGATCA
BglI 523 GCCGACAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4790 GCCTGGGAGGC
BglII 1271 AGATCT A^GATCT
2010 AGATCT
BsaAI 1579 CACGTA [CT]AC^GT[GA]
2745 TACGTA
BsaHI 1587 GGCGCC G[GA]^CG[CT]C
4739 GGCGCC
BsgI 2016 CTGCAC GTGCAG(16/14)
2901 GTGCAG
BsrFI 281 ACCGGC [GA]^CCGG[CT]
1779 GCCGGC
Bsu36I 162 CCTGAGG CC^T[ACGT]AGG
1571 CCTCAGG
Cfr10I 281 ACCGGC [GA]^CCGG[CT]
1779 GCCGGC
Eco81I 162 CCTGAGG CC^T[ACGT]AGG
1571 CCTCAGG
FbaI 582 TGATCA T^GATCA
812 TGATCA
Hin1I 1587 GGCGCC G[GA]^CG[CT]C
4739 GGCGCC
HincII 3333 GTTGAC GT[CT]^[GA]AC
3766 GTCAAC
HindII 3333 GTTGAC GT[CT]^[GA]AC
3766 GTCAAC
KasI 1587 GGCGCC G^GCGCC
4739 GGCGCC
MluNI 906 TGGCCA TGG^CCA
1518 TGGCCA
MscI 906 TGGCCA TGG^CCA
1518 TGGCCA
NarI 1587 GGCGCC GG^CGCC
4739 GGCGCC
PflFI 2871 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4858 GACTCCGTC
PvuII 1535 CAGCTG CAG^CTG
2321 CAGCTG
SfoI 1587 GGCGCC GGC^GCC
4739 GGCGCC
SphI 574 GCATGC GCATG^C
2999 GCATGC
Tth111I 2871 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4858 GACTCCGTC
XmnI 2793 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3503 GAACAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccIII AciI AcsI AfaI AflIII
AluI AlwI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseAI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspEI BspLU11I
BsrDI BsrI BssKI BssSI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MroI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NspI PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII