##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AccIII AflII Aor51HI
AscI AseI AsnI Asp718I AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BfrI BlnI BlpI Bpu1102I
BsaAI BseAI BsiWI BspEI Bst1107I
BstZ17I CelII CpoI DrdI Eco47III
EcoNI EcoRV FspI HindIII KpnI
MroI NdeI NotI NruI PacI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SalI SapI SbfI
SexAI SfiI SnaBI SpeI SphI
Sse8387I StuI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AclI 1378 AACGTT AA^CGTT
AgeI 16 ACCGGT A^CCGGT
AhdI 917 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2397 GTGCAC G^TGCAC
AocI 624 CCTCAGG CC^T[ACGT]AGG
ApaI 388 GGGCCC GGGCC^C
ApaLI 2397 GTGCAC G^TGCAC
AspEI 917 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1316 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1008 TGGCCA TGG^CCA
BbeI 553 GGCGCC GGCGC^C
BclI 1891 TGATCA T^GATCA
BglI 545 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2277 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 16 ACCGGT [AT]^CCGG[AT]
BsmBI 581 GAGACG CGTCTC(1/5)
BspDI 1167 ATCGAT AT^CGAT
BspHI 2762 TCATGA T^CATGA
BspLU11I 2323 ACATGT A^CATGT
BspMI 285 ACCTGC ACCTGC(4/8)
BsrBI 225 GAGCGG CCGCTC(-3/-3)
BsrDI 1132 CATTGC GCAATG(2/0)
BssHII 35 GCGCGC G^CGCGC
BssSI 2476 CTCGTG CACGAG(-5/-1)
Bsu36I 624 CCTCAGG CC^T[ACGT]AGG
ClaI 1167 ATCGAT AT^CGAT
DraIII 346 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 917 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 624 CCTCAGG CC^T[ACGT]AGG
EcoRI 1791 GAATTC G^AATTC
FbaI 1891 TGATCA T^GATCA
FseI 378 GGCCGGCC GGCCGG^CC
HpaI 2792 GTTAAC GTT^AAC
KasI 553 GGCGCC G^GCGCC
KspI 384 CCGCGG CCGC^GG
MamI 2277 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 588 ACGCGT A^CGCGT
MluNI 1008 TGGCCA TGG^CCA
MscI 1008 TGGCCA TGG^CCA
NarI 553 GGCGCC GG^CGCC
NheI 2519 GCTAGC G^CTAGC
PaeR7I 3560 CTCGAG C^TCGAG
PflFI 1316 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1571 CCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 16 ACCGGT A^CCGGT
PpuMI 282 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1378 AACGTT AA^CGTT
PvuII 1496 CAGCTG CAG^CTG
RcaI 2762 TCATGA T^CATGA
SacI 3656 GAGCTC GAGCT^C
SacII 384 CCGCGG CCGC^GG
ScaI 1588 AGTACT AGT^ACT
SfoI 553 GGCGCC GGC^GCC
SgrAI 304 CGCCGGCG C[GA]^CCGG[CT]G
SspI 2468 AATATT AAT^ATT
SwaI 2278 ATTTAAAT ATTT^AAAT
Tth111I 1316 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1571 CCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2006 TCTAGA T^CTAGA
XcmI 3837 CCATTACCTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3560 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 3077 GTCTAC GT^[AC][GT]AC
4044 GTAGAC
AcyI 357 GGCGTC G[GA]^CG[CT]C
553 GGCGCC
AlwNI 491 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
647 CAGGGTCTG
Asp700I 1601 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3902 GAAGCTGTTC
BglII 483 AGATCT A^GATCT
841 AGATCT
BsaHI 357 GGCGTC G[GA]^CG[CT]C
553 GGCGCC
BsaI 1752 GGTCTC GGTCTC(1/5)
2262 GGTCTC
Bsp1407I 1046 TGTACA T^GTACA
2615 TGTACA
BsrGI 1046 TGTACA T^GTACA
2615 TGTACA
BstBI 1608 TTCGAA TT^CGAA
1956 TTCGAA
BstEII 343 GGTCACC G^GT[ACGT]ACC
2862 GGTGACC
BstPI 343 GGTCACC G^GT[ACGT]ACC
2862 GGTGACC
BstXI 1530 CCAGTGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1728 CCAATGTTATGG
DraII 282 GGGACCT [GA]G^G[ACGT]CC[CT]
459 GGGGCCT
EcoO109I 282 GGGACCT [GA]G^G[ACGT]CC[CT]
459 GGGGCCT
EcoO65I 343 GGTCACC G^GT[ACGT]ACC
2862 GGTGACC
EcoT22I 1628 ATGCAT ATGCA^T
2591 ATGCAT
HaeII 230 GGCGCT [GA]GCGC^[CT]
553 GGCGCC
HgaI 587 GACGC GACGC(5/10)
358 GCGTC
Hin1I 357 GGCGTC G[GA]^CG[CT]C
553 GGCGCC
HincII 880 GTTGAC GT[CT]^[GA]AC
2792 GTTAAC
HindII 880 GTTGAC GT[CT]^[GA]AC
2792 GTTAAC
MfeI 687 CAATTG C^AATTG
1263 CAATTG
MunI 687 CAATTG C^AATTG
1263 CAATTG
NsiI 1628 ATGCAT ATGCA^T
2591 ATGCAT
NspV 1608 TTCGAA TT^CGAA
1956 TTCGAA
PstI 2031 CTGCAG CTGCA^G
3160 CTGCAG
SfuI 1608 TTCGAA TT^CGAA
1956 TTCGAA
SmaI 524 CCCGGG CCC^GGG
575 CCCGGG
SspBI 1046 TGTACA T^GTACA
2615 TGTACA
XmaCI 524 CCCGGG C^CCGGG
575 CCCGGG
XmaI 524 CCCGGG C^CCGGG
575 CCCGGG
XmnI 1601 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3902 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DsaI EaeI EagI
EarI EclXI Eco52I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII