##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AgeI AocI ApaI AscI AseI
AsnI AspI AvrII BbeI BbrPI
BclI BlnI BsaHI BseAI BsiWI
BspDI BspEI BspHI BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
Eco81I EcoRI FbaI FseI Hin1I
HindIII HpaI KasI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NspV PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 1539 GGTACC G^GTACC
AflII 2209 CTTAAG C^TTAAG
Alw44I 743 GTGCAC G^TGCAC
Aor51HI 1917 AGCGCT AGC^GCT
ApaLI 743 GTGCAC G^TGCAC
Asp718I 1539 GGTACC G^GTACC
AviII 624 TGCGCA TGC^GCA
BamHI 2362 GGATCC G^GATCC
BbvCI 4046 CCTCAGC CCTCAGC(-5/-2)
BciVI 1724 GTATCC GTATCC(6/5)
BfrI 2209 CTTAAG C^TTAAG
BglII 852 AGATCT A^GATCT
BsaAI 1589 TACGTG [CT]AC^GT[GA]
BsaBI 1294 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 127 ACCGGA [AT]^CCGG[AT]
BstEII 1567 GGTCACC G^GT[ACGT]ACC
BstPI 1567 GGTCACC G^GT[ACGT]ACC
EagI 1561 CGGCCG C^GGCCG
EclXI 1561 CGGCCG C^GGCCG
Eco47III 1917 AGCGCT AGC^GCT
Eco52I 1561 CGGCCG C^GGCCG
EcoO65I 1567 GGTCACC G^GT[ACGT]ACC
EcoRV 1703 GATATC GAT^ATC
EcoT22I 3142 ATGCAT ATGCA^T
FspI 624 TGCGCA TGC^GCA
HincII 2461 GTCAAC GT[CT]^[GA]AC
HindII 2461 GTCAAC GT[CT]^[GA]AC
KpnI 1539 GGTACC GGTAC^C
MamI 1294 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 226 GCCGGC GCC^GGC
NgoMIV 226 GCCGGC G^CCGGC
NsiI 3142 ATGCAT ATGCA^T
PacI 3068 TTAATTAA TTAAT^TAA
PaeR7I 529 CTCGAG C^TCGAG
ScaI 1599 AGTACT AGT^ACT
SexAI 1434 ACCTGGT A^CC[AT]GGT
SfiI 2263 GGCCCCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XbaI 4516 TCTAGA T^CTAGA
XcmI 1299 CCATCCGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 529 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AhdI 1128 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2547 GACATGCAGTC
AspEI 1128 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2547 GACATGCAGTC
BanII 97 GAGCCC G[GA]GC[CT]^C
694 GGGCTC
BcgI 2214 GCAGGCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4550 GCAATATGTTCG
BsiEI 84 CGGTCG CG[GA][CT]^CG
1561 CGGCCG
Bsp1407I 1092 TGTACA T^GTACA
2927 TGTACA
BspLU11I 3096 ACATGT A^CATGT
3936 ACATGT
BspMI 1235 ACCTGC ACCTGC(4/8)
1392 ACCTGC
BsrBI 776 CCGCTC CCGCTC(-3/-3)
2256 CCGCTC
BsrDI 3595 CATTGC GCAATG(2/0)
4421 CATTGC
BsrGI 1092 TGTACA T^GTACA
2927 TGTACA
DrdI 1868 GACTGCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2452 GACATTCTTGTC
Eam1105I 1128 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2547 GACATGCAGTC
PflMI 838 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1654 CCACGGTGTGG
PshAI 2418 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3443 GACTCTGGTC
SphI 621 GCATGC GCATG^C
3140 GCATGC
SspBI 1092 TGTACA T^GTACA
2927 TGTACA
Van91I 838 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1654 CCACGGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BsrFI BsrI
BssHII BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI XmnI