##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AclI AgeI AscI AseI
AsnI Asp718I AvrII BbeI BglII
BlnI BsiWI Bsp1407I BspDI BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI EcoRI EcoRV EcoT22I FseI
HindIII HpaI KasI KpnI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI SalI SfoI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2437 GACGTC GACGT^C
AccII 3031 CGCG CG^CG
AflII 3200 CTTAAG C^TTAAG
AocI 1492 CCTGAGG CC^T[ACGT]AGG
BciVI 1557 GGATAC GTATCC(6/5)
BclI 2108 TGATCA T^GATCA
BfrI 3200 CTTAAG C^TTAAG
BsaBI 3227 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2772 CGTCTC CGTCTC(1/5)
BsmI 381 GAATGC GAATGC(1/-1)
BspHI 1790 TCATGA T^CATGA
BspLU11I 3882 ACATGT A^CATGT
BstEII 945 GGTCACC G^GT[ACGT]ACC
BstPI 945 GGTCACC G^GT[ACGT]ACC
BstUI 3031 CGCG CG^CG
Bsu36I 1492 CCTGAGG CC^T[ACGT]AGG
DraI 3867 TTTAAA TTT^AAA
EagI 3033 CGGCCG C^GGCCG
EclXI 3033 CGGCCG C^GGCCG
Eco52I 3033 CGGCCG C^GGCCG
Eco81I 1492 CCTGAGG CC^T[ACGT]AGG
EcoO65I 945 GGTCACC G^GT[ACGT]ACC
FbaI 2108 TGATCA T^GATCA
HincII 3763 GTTGAC GT[CT]^[GA]AC
HindII 3763 GTTGAC GT[CT]^[GA]AC
KspI 3030 CCGCGG CCGC^GG
MamI 3227 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 3031 CGCG CG^CG
PaeR7I 931 CTCGAG C^TCGAG
RcaI 1790 TCATGA T^CATGA
SacI 928 GAGCTC GAGCT^C
SacII 3030 CCGCGG CCGC^GG
SbfI 1453 CCTGCAGG CCTGCA^GG
SfiI 2577 GGCCGAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1453 CCTGCAGG CCTGCA^GG
StuI 779 AGGCCT AGG^CCT
XhoI 931 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1765 GTAGAC GT^[AC][GT]AC
1906 GTCTAC
AflIII 1799 ACGTGT A^C[GA][CT]GT
3882 ACATGT
AhdI 571 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1978 GACTATGTGTC
ApaI 2277 GGGCCC GGGCC^C
3464 GGGCCC
Asp700I 810 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2967 GAAGAAATTC
AspEI 571 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1978 GACTATGTGTC
AviII 3543 TGCGCA TGC^GCA
3574 TGCGCA
BbsI 538 GAAGAC GAAGAC(2/6)
2379 GAAGAC
BbvCI 1696 GCTGAGG CCTCAGC(-5/-2)
3059 GCTGAGG
BlpI 1291 GCTGAGC GC^T[ACGT]AGC
2397 GCTCAGC
Bpu1102I 1291 GCTGAGC GC^T[ACGT]AGC
2397 GCTCAGC
BpuAI 538 GAAGAC GAAGAC(2/6)
2379 GAAGAC
BsiEI 2541 CGACCG CG[GA][CT]^CG
3033 CGGCCG
BsrBI 747 CCGCTC CCGCTC(-3/-3)
2733 CCGCTC
BssSI 1563 CACGAG CACGAG(-5/-1)
202 CTCGTG
CelII 1291 GCTGAGC GC^T[ACGT]AGC
2397 GCTCAGC
CpoI 1315 CGGACCG CG^G[AT]CCG
2426 CGGACCG
DrdI 423 GACCTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1897 GACTGCTATGTC
Eam1105I 571 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1978 GACTATGTGTC
FspI 3543 TGCGCA TGC^GCA
3574 TGCGCA
MseI 3201 TTAA T^TAA
3868 TTAA
MslI 858 CACCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1203 CACCTCCATG
NaeI 828 GCCGGC GCC^GGC
3112 GCCGGC
NgoMIV 828 GCCGGC G^CCGGC
3112 GCCGGC
RsrII 1315 CGGACCG CG^G[AT]CCG
2426 CGGACCG
SapI 1816 GAAGAGC GCTCTTC(1/4)
414 GCTCTTC
ScaI 368 AGTACT AGT^ACT
2795 AGTACT
SexAI 1276 ACCTGGT A^CC[AT]GGT
1943 ACCTGGT
SmaI 1327 CCCGGG CCC^GGG
3340 CCCGGG
SphI 2330 GCATGC GCATG^C
3644 GCATGC
TfiI 1702 GATTC G^A[AT]TC
2197 GAATC
Tru9I 3201 TTAA T^TAA
3868 TTAA
XmaCI 1327 CCCGGG C^CCGGG
3340 CCCGGG
XmaI 1327 CCCGGG C^CCGGG
3340 CCCGGG
XmnI 810 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2967 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccIII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI Aor51HI
ApaLI ApoI AspHI AspI AvaI
AvaII BalI BamHI BanI BanII
BbrPI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco47III
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII