##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
Aor51HI ApaI AscI AspI AviII
AvrII BalI BamHI BbrPI BbvCI
BglI BlnI BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspEI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Cfr10I CpoI DraIII EaeI
EagI EclXI Eco47III Eco52I EcoNI
EcoRI FseI FspI KspI MamI
MluI MluNI MroI MscI MspA1I
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SfiI SfuI
SgrAI SnaBI SspBI SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 1466 GGTACC G^GTACC
AccI 2268 GTAGAC GT^[AC][GT]AC
AflII 3135 CTTAAG C^TTAAG
AhdI 2148 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2871 CCTGAGG CC^T[ACGT]AGG
AseI 3170 ATTAAT AT^TAAT
AsnI 3170 ATTAAT AT^TAAT
Asp700I 429 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1466 GGTACC G^GTACC
AspEI 2148 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 726 GAGCCC G[GA]GC[CT]^C
BbeI 1916 GGCGCC GGCGC^C
BbsI 3674 GTCTTC GAAGAC(2/6)
BfrI 3135 CTTAAG C^TTAAG
BpuAI 3674 GTCTTC GAAGAC(2/6)
BspDI 851 ATCGAT AT^CGAT
BspHI 502 TCATGA T^CATGA
BspLU11I 2994 ACATGT A^CATGT
BstAPI 1117 GCAATTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 173 GGTCACC G^GT[ACGT]ACC
BstPI 173 GGTCACC G^GT[ACGT]ACC
Bsu36I 2871 CCTGAGG CC^T[ACGT]AGG
ClaI 851 ATCGAT AT^CGAT
DrdI 1506 GACCCCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2148 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2871 CCTGAGG CC^T[ACGT]AGG
EcoO65I 173 GGTCACC G^GT[ACGT]ACC
EcoRV 46 GATATC GAT^ATC
EcoT22I 4069 ATGCAT ATGCA^T
FauI 1803 GCGGG CCCGC(4/6)
HaeII 1916 GGCGCC [GA]GCGC^[CT]
KasI 1916 GGCGCC G^GCGCC
KpnI 1466 GGTACC GGTAC^C
MfeI 1859 CAATTG C^AATTG
MunI 1859 CAATTG C^AATTG
NarI 1916 GGCGCC GG^CGCC
NsiI 4069 ATGCAT ATGCA^T
PflMI 887 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3170 ATTAAT AT^TAAT
RcaI 502 TCATGA T^CATGA
SapI 2056 GCTCTTC GCTCTTC(1/4)
SbfI 1897 CCTGCAGG CCTGCA^GG
ScaI 2590 AGTACT AGT^ACT
SexAI 3571 ACCAGGT A^CC[AT]GGT
SfoI 1916 GGCGCC GGC^GCC
SmaI 1912 CCCGGG CCC^GGG
SphI 2249 GCATGC GCATG^C
Sse8387I 1897 CCTGCAGG CCTGCA^GG
SspI 2714 AATATT AAT^ATT
Van91I 887 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2613 TCTAGA T^CTAGA
XmaCI 1912 CCCGGG C^CCGGG
XmaI 1912 CCCGGG C^CCGGG
XmnI 429 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 1448 GACGCC G[GA]^CG[CT]C
1916 GGCGCC
Alw44I 320 GTGCAC G^TGCAC
3601 GTGCAC
AlwNI 2131 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2892 CAGACACTG
ApaLI 320 GTGCAC G^TGCAC
3601 GTGCAC
AvaI 1912 CCCGGG C^[CT]CG[GA]G
2150 CTCGGG
BanI 1466 GGTACC G^G[CT][GA]CC
1916 GGCGCC
BcgI 1793 CGAACACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
185 GCACTAACATCG
BciVI 1833 GGATAC GTATCC(6/5)
684 GTATCC
BclI 1339 TGATCA T^GATCA
1565 TGATCA
BglII 490 AGATCT A^GATCT
2068 AGATCT
BlpI 741 GCTGAGC GC^T[ACGT]AGC
3723 GCTCAGC
Bpu1102I 741 GCTGAGC GC^T[ACGT]AGC
3723 GCTCAGC
BsaHI 1448 GACGCC G[GA]^CG[CT]C
1916 GGCGCC
BsoBI 1912 CCCGGG C^[CT]CG[GA]G
2150 CTCGGG
BspMI 1345 ACCTGC ACCTGC(4/8)
3754 GCAGGT
BsrDI 472 GCAATG GCAATG(2/0)
4061 GCAATG
CelII 741 GCTGAGC GC^T[ACGT]AGC
3723 GCTCAGC
CfoI 1917 GCGC GCG^C
1974 GCGC
DraII 2154 GGGTCCC [GA]G^G[ACGT]CC[CT]
3756 AGGTCCC
EcoO109I 2154 GGGTCCC [GA]G^G[ACGT]CC[CT]
3756 AGGTCCC
FbaI 1339 TGATCA T^GATCA
1565 TGATCA
HapII 1913 CCGG C^CGG
2273 CCGG
HgaI 1448 GACGC GACGC(5/10)
3027 GCGTC
HhaI 1917 GCGC GCG^C
1974 GCGC
Hin1I 1448 GACGCC G[GA]^CG[CT]C
1916 GGCGCC
HinP1I 1917 GCGC G^CGC
1974 GCGC
HpaII 1913 CCGG C^CGG
2273 CCGG
MspI 1913 CCGG C^CGG
2273 CCGG
PpuMI 2154 GGGTCCC [GA]G^G[AT]CC[CT]
3756 AGGTCCC
XcmI 1453 CCAACAAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3293 CCACCAAACTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI HpaI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SpeI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII