##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Aor51HI AscI Asp718I
AspEI BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bpu1102I
BsaBI BseAI BsiEI BsiWI BspDI
BspEI BspHI BsrBI BsrFI BssHII
Bst1107I BstBI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FbaI FseI HpaI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmeI Psp1406I PvuI RcaI RsrII
SacII SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 2279 GTCGAC GT^[AC][GT]AC
AflII 481 CTTAAG C^TTAAG
Alw44I 2854 GTGCAC G^TGCAC
AlwNI 3514 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1710 CCTGAGG CC^T[ACGT]AGG
ApaLI 2854 GTGCAC G^TGCAC
AspI 1816 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2971 CTCGAG C^[CT]CG[GA]G
AviII 1916 TGCGCA TGC^GCA
BalI 1027 TGGCCA TGG^CCA
BamHI 3378 GGATCC G^GATCC
BanI 2013 GGCACC G^G[CT][GA]CC
BbrPI 2851 CACGTG CAC^GTG
BfrI 481 CTTAAG C^TTAAG
BsaI 895 GAGACC GGTCTC(1/5)
BsaWI 2534 ACCGGA [AT]^CCGG[AT]
BsmBI 82 GAGACG CGTCTC(1/5)
BsoBI 2971 CTCGAG C^[CT]CG[GA]G
BssSI 2384 CTCGTG CACGAG(-5/-1)
BstAPI 2425 GCAGGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2349 GGTTACC G^GT[ACGT]ACC
BstPI 2349 GGTTACC G^GT[ACGT]ACC
BstXI 906 CCACTTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1710 CCTGAGG CC^T[ACGT]AGG
DraIII 1233 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1027 TGGCCA [CT]^GGCC[GA]
Eco81I 1710 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2349 GGTTACC G^GT[ACGT]ACC
FauI 3584 GCGGG CCCGC(4/6)
FspI 1916 TGCGCA TGC^GCA
HaeII 64 GGCGCT [GA]GCGC^[CT]
HindIII 1157 AAGCTT A^AGCTT
MfeI 176 CAATTG C^AATTG
MluNI 1027 TGGCCA TGG^CCA
MscI 1027 TGGCCA TGG^CCA
MunI 176 CAATTG C^AATTG
PaeR7I 2971 CTCGAG C^TCGAG
PflFI 1816 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2632 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2851 CACGTG CAC^GTG
PmlI 2851 CACGTG CAC^GTG
PpuMI 920 AGGACCT [GA]G^G[AT]CC[CT]
SacI 787 GAGCTC GAGCT^C
SalI 2279 GTCGAC G^TCGAC
SexAI 1817 ACCTGGT A^CC[AT]GGT
Tth111I 1816 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2632 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 561 TCTAGA T^CTAGA
XhoI 2971 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 1675 GACGCC G[GA]^CG[CT]C
2153 GGCGTC
AflIII 820 ACATGT A^C[GA][CT]GT
3524 ACATGT
ApaI 2055 GGGCCC GGGCC^C
2564 GGGCCC
AseI 427 ATTAAT AT^TAAT
3260 ATTAAT
AsnI 427 ATTAAT AT^TAAT
3260 ATTAAT
AvrII 2484 CCTAGG C^CTAGG
3923 CCTAGG
BlnI 2484 CCTAGG C^CTAGG
3923 CCTAGG
BsaHI 1675 GACGCC G[GA]^CG[CT]C
2153 GGCGTC
BspLU11I 820 ACATGT A^CATGT
3524 ACATGT
BspMI 3660 ACCTGC ACCTGC(4/8)
3683 GCAGGT
BstYI 1413 GGATCT [GA]^GATC[CT]
3378 GGATCC
CfoI 65 GCGC GCG^C
1917 GCGC
EcoNI 207 CCTAATAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2731 CCTGGGGGAGG
EcoRI 369 GAATTC G^AATTC
992 GAATTC
EcoT22I 443 ATGCAT ATGCA^T
1022 ATGCAT
HgaI 1675 GACGC GACGC(5/10)
2154 GCGTC
HhaI 65 GCGC GCG^C
1917 GCGC
Hin1I 1675 GACGCC G[GA]^CG[CT]C
2153 GGCGTC
HinP1I 65 GCGC G^CGC
1917 GCGC
MflI 1413 GGATCT [GA]^GATC[CT]
3378 GGATCC
MspA1I 793 CAGCTG C[AC]G^C[GT]G
1967 CAGCTG
NcoI 912 CCATGG C^CATGG
3647 CCATGG
NsiI 443 ATGCAT ATGCA^T
1022 ATGCAT
PshAI 3473 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3645 GACCATGGTC
PshBI 427 ATTAAT AT^TAAT
3260 ATTAAT
PvuII 793 CAGCTG CAG^CTG
1967 CAGCTG
SphI 2867 GCATGC GCATG^C
3549 GCATGC
SspI 313 AATATT AAT^ATT
4009 AATATT
XcmI 2628 CCACCCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2697 CCATGAGAAAAATGG
XhoII 1413 GGATCT [GA]^GATC[CT]
3378 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI