##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AlwNI AocI Aor51HI ApaI AscI
Asp718I AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BciVI BclI
BcnI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BsrBI BsrFI
BssHII BssSI BstAPI BstBI Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoRV
FbaI FseI FspI HapII HgaI
Hin1I HpaII KasI KpnI KspI
MluI MroI MspI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PstI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 245 GTATAC GT^[AC][GT]AC
AccII 22 CGCG CG^CG
AclI 2862 AACGTT AA^CGTT
AflII 3476 CTTAAG C^TTAAG
AflIII 3284 ACATGT A^C[GA][CT]GT
AhdI 169 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 169 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1191 TGGCCA TGG^CCA
BanI 1426 GGTGCC G^G[CT][GA]CC
BanII 1002 GGGCTC G[GA]GC[CT]^C
BcgI 1225 CGAAGCAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3476 CTTAAG C^TTAAG
BglI 9 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3472 AGATCT A^GATCT
BpmI 1884 CTGGAG CTGGAG(16/14)
BsaBI 693 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2007 GGTCTC GGTCTC(1/5)
BsmI 657 GCATTC GAATGC(1/-1)
BspLU11I 3284 ACATGT A^CATGT
BspMI 271 GCAGGT ACCTGC(4/8)
BsrDI 1074 CATTGC GCAATG(2/0)
Bst1107I 245 GTATAC GTA^TAC
BstEII 186 GGTTACC G^GT[ACGT]ACC
BstPI 186 GGTTACC G^GT[ACGT]ACC
BstUI 22 CGCG CG^CG
BstXI 1615 CCAAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3472 AGATCT [GA]^GATC[CT]
BstZ17I 245 GTATAC GTA^TAC
EaeI 1191 TGGCCA [CT]^GGCC[GA]
Eam1105I 169 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1729 CTCTTC CTCTTC(1/4)
EcoNI 2684 CCTCTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 186 GGTTACC G^GT[ACGT]ACC
EcoT22I 655 ATGCAT ATGCA^T
HaeII 3118 AGCGCC [GA]GCGC^[CT]
HpaI 3280 GTTAAC GTT^AAC
Ksp632I 1729 CTCTTC CTCTTC(1/4)
MamI 693 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1230 CAATTG C^AATTG
MflI 3472 AGATCT [GA]^GATC[CT]
MluNI 1191 TGGCCA TGG^CCA
MscI 1191 TGGCCA TGG^CCA
MslI 802 CACTACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1230 CAATTG C^AATTG
MvnI 22 CGCG CG^CG
NdeI 476 CATATG CA^TATG
NsiI 655 ATGCAT ATGCA^T
Psp1406I 2862 AACGTT AA^CGTT
SapI 1728 GCTCTTC GCTCTTC(1/4)
ScaI 2836 AGTACT AGT^ACT
SfcI 3224 CTGTAG C^T[GA][CT]AG
SpeI 514 ACTAGT A^CTAGT
StuI 2681 AGGCCT AGG^CCT
XhoII 3472 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 799 GTGCAC G^TGCAC
2462 GTGCAC
ApaLI 799 GTGCAC G^TGCAC
2462 GTGCAC
AvaI 702 CTCGAG C^[CT]CG[GA]G
836 CTCGAG
BsoBI 702 CTCGAG C^[CT]CG[GA]G
836 CTCGAG
Bsp1407I 828 TGTACA T^GTACA
3064 TGTACA
BspHI 2375 TCATGA T^CATGA
2434 TCATGA
BsrGI 828 TGTACA T^GTACA
3064 TGTACA
HincII 3280 GTTAAC GT[CT]^[GA]AC
3394 GTCAAC
HindII 3280 GTTAAC GT[CT]^[GA]AC
3394 GTCAAC
HphI 313 GGTGA GGTGA(8/7)
1696 GGTGA
MaeII 155 ACGT A^CGT
2863 ACGT
NlaIV 860 GGTCCC GG[ACGT]^[ACGT]CC
1426 GGTGCC
NspI 1323 GCATGT [GA]CATG^[CT]
3284 ACATGT
PaeR7I 702 CTCGAG C^TCGAG
836 CTCGAG
RcaI 2375 TCATGA T^CATGA
2434 TCATGA
SspBI 828 TGTACA T^GTACA
3064 TGTACA
SwaI 2964 ATTTAAAT ATTT^AAAT
3355 ATTTAAAT
XbaI 1245 TCTAGA T^CTAGA
2702 TCTAGA
XhoI 702 CTCGAG C^TCGAG
836 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AspHI
AvaII BbsI BbvI BfaI BmyI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HhaI HinP1I HindIII
HinfI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII PflMI PleI
PshBI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XmnI