##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AclI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI BalI BbeI BcgI
BglII BsiWI BspLU11I BssHII Bst1107I
BstBI BstZ17I CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoT22I FseI
HpaI KasI KpnI KspI MfeI
MluI MluNI MscI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1859 GACGTC GACGT^C
AflII 4437 CTTAAG C^TTAAG
AflIII 2014 ACACGT A^C[GA][CT]GT
AgeI 2779 ACCGGT A^CCGGT
AvrII 2135 CCTAGG C^CTAGG
BamHI 1397 GGATCC G^GATCC
BbrPI 3570 CACGTG CAC^GTG
BfrI 4437 CTTAAG C^TTAAG
BlnI 2135 CCTAGG C^CTAGG
BlpI 346 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 346 GCTCAGC GC^T[ACGT]AGC
BsiEI 3018 CGGTCG CG[GA][CT]^CG
BspDI 247 ATCGAT AT^CGAT
BsrDI 2502 GCAATG GCAATG(2/0)
BssSI 3891 CACGAG CACGAG(-5/-1)
BstEII 406 GGTAACC G^GT[ACGT]ACC
BstPI 406 GGTAACC G^GT[ACGT]ACC
CelII 346 GCTCAGC GC^T[ACGT]AGC
ClaI 247 ATCGAT AT^CGAT
DraIII 1956 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1438 CGGCCA [CT]^GGCC[GA]
EcoO65I 406 GGTAACC G^GT[ACGT]ACC
HaeII 1888 GGCGCT [GA]GCGC^[CT]
HincII 2292 GTCAAC GT[CT]^[GA]AC
HindII 2292 GTCAAC GT[CT]^[GA]AC
HindIII 220 AAGCTT A^AGCTT
NcoI 1949 CCATGG C^CATGG
NdeI 1053 CATATG CA^TATG
PflMI 699 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2779 ACCGGT A^CCGGT
PmaCI 3570 CACGTG CAC^GTG
PmlI 3570 CACGTG CAC^GTG
SexAI 2431 ACCTGGT A^CC[AT]GGT
SfiI 3806 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 4099 AGGCCT AGG^CCT
SwaI 807 ATTTAAAT ATTT^AAAT
Van91I 699 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1457 GTCTAC GT^[AC][GT]AC
3001 GTAGAC
AccIII 3746 TCCGGA T^CCGGA
3922 TCCGGA
AcyI 1859 GACGTC G[GA]^CG[CT]C
4239 GACGCC
AocI 2265 CCTAAGG CC^T[ACGT]AGG
3189 CCTGAGG
ApaI 2369 GGGCCC GGGCC^C
3797 GGGCCC
AspI 744 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3344 GACAGAGTC
BbvCI 3006 CCTCAGC CCTCAGC(-5/-2)
4166 GCTGAGG
BciVI 385 GTATCC GTATCC(6/5)
483 GTATCC
BglI 1880 GCCAGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3807 GCCCAGCAGGC
BsaBI 1934 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1940 GATCTTCATC
BsaHI 1859 GACGTC G[GA]^CG[CT]C
4239 GACGCC
BseAI 3746 TCCGGA T^CCGGA
3922 TCCGGA
BsmBI 1857 GAGACG CGTCTC(1/5)
4143 GAGACG
BsmI 1628 GAATGC GAATGC(1/-1)
3846 GAATGC
Bsp1407I 1990 TGTACA T^GTACA
2005 TGTACA
BspEI 3746 TCCGGA T^CCGGA
3922 TCCGGA
BspHI 997 TCATGA T^CATGA
1580 TCATGA
BsrBI 2930 CCGCTC CCGCTC(-3/-3)
1884 GAGCGG
BsrFI 2485 ACCGGC [GA]^CCGG[CT]
2779 ACCGGT
BsrGI 1990 TGTACA T^GTACA
2005 TGTACA
Bsu36I 2265 CCTAAGG CC^T[ACGT]AGG
3189 CCTGAGG
Cfr10I 2485 ACCGGC [GA]^CCGG[CT]
2779 ACCGGT
DrdI 3004 GACCTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3903 GACCTGCTTGTC
DsaI 1949 CCATGG C^C[GA][CT]GG
3619 CCGTGG
Eco81I 2265 CCTAAGG CC^T[ACGT]AGG
3189 CCTGAGG
EcoNI 4123 CCTCCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4190 CCTGCAAAAGG
EcoRI 1257 GAATTC G^AATTC
3989 GAATTC
Hin1I 1859 GACGTC G[GA]^CG[CT]C
4239 GACGCC
MamI 1934 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1940 GATCTTCATC
MroI 3746 TCCGGA T^CCGGA
3922 TCCGGA
PflFI 744 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3344 GACAGAGTC
PpuMI 1345 AGGACCT [GA]G^G[AT]CC[CT]
4252 AGGACCT
PstI 4158 CTGCAG CTGCA^G
4328 CTGCAG
RcaI 997 TCATGA T^CATGA
1580 TCATGA
ScaI 1699 AGTACT AGT^ACT
4024 AGTACT
SspBI 1990 TGTACA T^GTACA
2005 TGTACA
Tth111I 744 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3344 GACAGAGTC
XcmI 1614 CCACTGCCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3687 CCAAGAGACCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII AviII BanI BanII BbsI
BbvI BclI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoRV
EcoT14I FauI FbaI Fnu4HI FokI
FspI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI