##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
ApaI AscI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbvCI
BcnI BglI BlnI BlpI Bpu1102I
BsiWI BssHII BstEII BstPI CelII
CpoI DraII DrdI EagI Eam1105I
EclXI Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI KasI
KpnI KspI MfeI MluI MunI
NarI NciI NheI NotI NruI
NsiI PaeR7I PflFI PinAI PmeI
PpuMI PshAI Psp1406I PstI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SmaI SnaBI SphI Sse8387I
StuI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccII 1830 CGCG CG^CG
AccIII 434 TCCGGA T^CCGGA
AcyI 1406 GACGCC G[GA]^CG[CT]C
Alw44I 697 GTGCAC G^TGCAC
AocI 3830 CCTGAGG CC^T[ACGT]AGG
Aor51HI 370 AGCGCT AGC^GCT
ApaLI 697 GTGCAC G^TGCAC
AseI 3471 ATTAAT AT^TAAT
AsnI 3471 ATTAAT AT^TAAT
BanI 2736 GGCACC G^G[CT][GA]CC
BcgI 1465 GCAGCAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1652 TGATCA T^GATCA
BsaHI 1406 GACGCC G[GA]^CG[CT]C
BsaWI 434 TCCGGA [AT]^CCGG[AT]
BseAI 434 TCCGGA T^CCGGA
BsiEI 98 CGATCG CG[GA][CT]^CG
BsmBI 1404 GAGACG CGTCTC(1/5)
Bsp1407I 3336 TGTACA T^GTACA
BspDI 1807 ATCGAT AT^CGAT
BspEI 434 TCCGGA T^CCGGA
BsrBI 105 GAGCGG CCGCTC(-3/-3)
BsrDI 1036 GCAATG GCAATG(2/0)
BsrFI 418 GCCGGC [GA]^CCGG[CT]
BsrGI 3336 TGTACA T^GTACA
Bst1107I 3938 GTATAC GTA^TAC
BstBI 2707 TTCGAA TT^CGAA
BstUI 1830 CGCG CG^CG
BstXI 1541 CCATCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3938 GTATAC GTA^TAC
Bsu36I 3830 CCTGAGG CC^T[ACGT]AGG
Cfr10I 418 GCCGGC [GA]^CCGG[CT]
ClaI 1807 ATCGAT AT^CGAT
DraIII 3316 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 370 AGCGCT AGC^GCT
Eco81I 3830 CCTGAGG CC^T[ACGT]AGG
FauI 107 GCGGG CCCGC(4/6)
FbaI 1652 TGATCA T^GATCA
HgaI 1406 GACGC GACGC(5/10)
Hin1I 1406 GACGCC G[GA]^CG[CT]C
HincII 3811 GTTAAC GT[CT]^[GA]AC
HindII 3811 GTTAAC GT[CT]^[GA]AC
HindIII 2749 AAGCTT A^AGCTT
HpaI 3811 GTTAAC GTT^AAC
MroI 434 TCCGGA T^CCGGA
MslI 1542 CATCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 3897 CAGCTG C[AC]G^C[GT]G
MvnI 1830 CGCG CG^CG
NaeI 418 GCCGGC GCC^GGC
NcoI 3238 CCATGG C^CATGG
NdeI 4193 CATATG CA^TATG
NgoMIV 418 GCCGGC G^CCGGC
NspV 2707 TTCGAA TT^CGAA
PacI 3468 TTAATTAA TTAAT^TAA
PflMI 336 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3471 ATTAAT AT^TAAT
PvuI 98 CGATCG CGAT^CG
PvuII 3897 CAGCTG CAG^CTG
SacI 751 GAGCTC GAGCT^C
SfuI 2707 TTCGAA TT^CGAA
SgrAI 417 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 3059 ACTAGT A^CTAGT
SspBI 3336 TGTACA T^GTACA
Van91I 336 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1757 CCATTCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 2772 GTAGAC GT^[AC][GT]AC
3938 GTATAC
AflIII 2541 ACATGT A^C[GA][CT]GT
3656 ACATGT
AvaI 1473 CTCGGG C^[CT]CG[GA]G
2116 CCCGAG
BanII 751 GAGCTC G[GA]GC[CT]^C
2602 GGGCTC
BbrPI 1413 CACGTG CAC^GTG
3345 CACGTG
BciVI 2277 GGATAC GTATCC(6/5)
2878 GTATCC
BglII 1764 AGATCT A^GATCT
2156 AGATCT
BsaAI 1413 CACGTG [CT]AC^GT[GA]
3345 CACGTG
BsaBI 327 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2151 GATAAAGATC
BsaI 85 GAGACC GGTCTC(1/5)
91 GAGACC
BsoBI 1473 CTCGGG C^[CT]CG[GA]G
2116 CCCGAG
BspHI 755 TCATGA T^CATGA
3973 TCATGA
BspLU11I 2541 ACATGT A^CATGT
3656 ACATGT
BstAPI 1248 GCAACTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2319 GCAGGTGATGC
DsaI 990 CCACGG C^C[GA][CT]GG
3238 CCATGG
EcoNI 4087 CCTCTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4224 CCTGCCAAAGG
EcoT14I 2131 CCTTGG C^C[AT][AT]GG
3238 CCATGG
HapII 419 CCGG C^CGG
435 CCGG
HpaII 419 CCGG C^CGG
435 CCGG
MamI 327 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2151 GATAAAGATC
MspI 419 CCGG C^CGG
435 CCGG
PmaCI 1413 CACGTG CAC^GTG
3345 CACGTG
PmlI 1413 CACGTG CAC^GTG
3345 CACGTG
RcaI 755 TCATGA T^CATGA
3973 TCATGA
SapI 3202 GCTCTTC GCTCTTC(1/4)
3605 GCTCTTC
SspI 3218 AATATT AAT^ATT
3840 AATATT
StyI 2131 CCTTGG C^C[AT][AT]GG
3238 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BbsI BbvI BfaI
BfrI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BssSI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI