##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BamHI BbeI BbsI BbvCI
BclI BfrI BglI BglII BlnI
BpuAI BsaHI BseAI BsiWI Bsp1407I
BspDI BspEI BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DrdI Eco47III EcoO65I
EcoRV FbaI FspI Hin1I HindIII
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NarI
NdeI NheI NotI NspV PacI
PflFI PinAI PmeI PshAI PshBI
RsrII SacII SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AclI 577 AACGTT AA^CGTT
AhdI 2848 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2389 GTGCAC G^TGCAC
ApaI 811 GGGCCC GGGCC^C
ApaLI 2389 GTGCAC G^TGCAC
AspEI 2848 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 732 CACGTG CAC^GTG
BcgI 2152 GCATGCATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2575 GGATAC GTATCC(6/5)
BlpI 939 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 939 GCTGAGC GC^T[ACGT]AGC
BsaBI 882 GATACGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1272 GAGACG CGTCTC(1/5)
BspLU11I 3334 ACATGT A^CATGT
BspMI 2562 GCAGGT ACCTGC(4/8)
BsrDI 3490 CATTGC GCAATG(2/0)
BssSI 1200 CACGAG CACGAG(-5/-1)
CelII 939 GCTGAGC GC^T[ACGT]AGC
DraIII 3286 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2848 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2290 GAATTC G^AATTC
FseI 563 GGCCGGCC GGCCGG^CC
HaeII 2873 GGCGCT [GA]GCGC^[CT]
HpaI 4137 GTTAAC GTT^AAC
MamI 882 GATACGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 3909 CCATGG C^CATGG
NruI 1094 TCGCGA TCG^CGA
PmaCI 732 CACGTG CAC^GTG
PmlI 732 CACGTG CAC^GTG
PpuMI 3777 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 577 AACGTT AA^CGTT
PvuI 3345 CGATCG CGAT^CG
SalI 346 GTCGAC G^TCGAC
SapI 2352 GCTCTTC GCTCTTC(1/4)
XbaI 3062 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 346 GTCGAC GT^[AC][GT]AC
3225 GTAGAC
AccII 286 CGCG CG^CG
1095 CGCG
AflIII 731 ACACGT A^C[GA][CT]GT
3334 ACATGT
BanI 183 GGTGCC G^G[CT][GA]CC
2600 GGTGCC
BcnI 953 CCCGG CC^[GC]GG
1494 CCGGG
BsaAI 732 CACGTG [CT]AC^GT[GA]
2174 TACGTG
BsaI 610 GGTCTC GGTCTC(1/5)
2072 GGTCTC
BsaWI 1420 ACCGGA [AT]^CCGG[AT]
2597 TCCGGT
BsrFI 564 GCCGGC [GA]^CCGG[CT]
3747 GCCGGC
BstUI 286 CGCG CG^CG
1095 CGCG
BstXI 553 CCAAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1720 CCAACAGCGTGG
BstYI 101 GGATCT [GA]^GATC[CT]
557 GGATCT
Cfr10I 564 GCCGGC [GA]^CCGG[CT]
3747 GCCGGC
DraII 810 AGGGCCC [GA]G^G[ACGT]CC[CT]
3777 GGGTCCT
DsaI 1073 CCGTGG C^C[GA][CT]GG
3909 CCATGG
EagI 566 CGGCCG C^GGCCG
1285 CGGCCG
EclXI 566 CGGCCG C^GGCCG
1285 CGGCCG
Eco52I 566 CGGCCG C^GGCCG
1285 CGGCCG
EcoO109I 810 AGGGCCC [GA]G^G[ACGT]CC[CT]
3777 GGGTCCT
HincII 346 GTCGAC GT[CT]^[GA]AC
4137 GTTAAC
HindII 346 GTCGAC GT[CT]^[GA]AC
4137 GTTAAC
MflI 101 GGATCT [GA]^GATC[CT]
557 GGATCT
MvnI 286 CGCG CG^CG
1095 CGCG
NaeI 564 GCCGGC GCC^GGC
3747 GCCGGC
NciI 953 CCCGG CC^[GC]GG
1494 CCGGG
NgoMIV 564 GCCGGC G^CCGGC
3747 GCCGGC
PaeR7I 326 CTCGAG C^TCGAG
659 CTCGAG
PflMI 12 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1262 CCAGGTGATGG
ScaI 2424 AGTACT AGT^ACT
3195 AGTACT
SphI 281 GCATGC GCATG^C
2152 GCATGC
SspI 3438 AATATT AAT^ATT
3956 AATATT
TfiI 1408 GATTC G^A[AT]TC
3526 GAATC
Van91I 12 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1262 CCAGGTGATGG
XcmI 1505 CCATCATCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1860 CCATGCTTTTGCTGG
XhoI 326 CTCGAG C^TCGAG
659 CTCGAG
XhoII 101 GGATCT [GA]^GATC[CT]
557 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BanII BbvI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BsrI
BssKI BstAPI BstF5I BstNI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI EaeI EarI Eco81I EcoNI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PleI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I