##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccIII AcsI AflII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BcgI BclI BfrI BglII BlnI
BsaBI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MamI
MfeI MroI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI PvuI
RcaI RsrII SbfI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 47 GACGTC GACGT^C
AclI 3914 AACGTT AA^CGTT
AviII 960 TGCGCA TGC^GCA
BbrPI 3838 CACGTG CAC^GTG
BlpI 2086 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2086 GCTGAGC GC^T[ACGT]AGC
BsaAI 3838 CACGTG [CT]AC^GT[GA]
BsmI 1938 GCATTC GAATGC(1/-1)
BssSI 2883 CACGAG CACGAG(-5/-1)
CelII 2086 GCTGAGC GC^T[ACGT]AGC
FspI 960 TGCGCA TGC^GCA
MluI 369 ACGCGT A^CGCGT
NaeI 265 GCCGGC GCC^GGC
NcoI 240 CCATGG C^CATGG
NgoMIV 265 GCCGGC G^CCGGC
NheI 2551 GCTAGC G^CTAGC
NspI 2104 GCATGT [GA]CATG^[CT]
PmaCI 3838 CACGTG CAC^GTG
PmlI 3838 CACGTG CAC^GTG
Psp1406I 3914 AACGTT AA^CGTT
SapI 1239 GCTCTTC GCTCTTC(1/4)
ScaI 2028 AGTACT AGT^ACT
SexAI 186 ACCAGGT A^CC[AT]GGT
SfiI 1480 GGCCCCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2185 CCCGGG CCC^GGG
SmlI 1774 CTTGAG C^T[CT][GA]AG
XmaCI 2185 CCCGGG C^CCGGG
XmaI 2185 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 599 GTCGAC GT^[AC][GT]AC
3143 GTCGAC
AflIII 369 ACGCGT A^C[GA][CT]GT
3837 ACACGT
Alw44I 2394 GTGCAC G^TGCAC
3587 GTGCAC
AocI 3413 CCTCAGG CC^T[ACGT]AGG
3827 CCTCAGG
ApaLI 2394 GTGCAC G^TGCAC
3587 GTGCAC
AspI 635 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
976 GACCCAGTC
BalI 1969 TGGCCA TGG^CCA
3278 TGGCCA
BamHI 895 GGATCC G^GATCC
3339 GGATCC
BbeI 526 GGCGCC GGCGC^C
1052 GGCGCC
BbvCI 1017 CCTCAGC CCTCAGC(-5/-2)
2454 CCTCAGC
BciVI 3739 GGATAC GTATCC(6/5)
3956 GTATCC
BspMI 2435 ACCTGC ACCTGC(4/8)
4213 GCAGGT
Bsu36I 3413 CCTCAGG CC^T[ACGT]AGG
3827 CCTCAGG
DraIII 667 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1814 CACCCCGTG
EagI 97 CGGCCG C^GGCCG
172 CGGCCG
EclXI 97 CGGCCG C^GGCCG
172 CGGCCG
Eco52I 97 CGGCCG C^GGCCG
172 CGGCCG
Eco81I 3413 CCTCAGG CC^T[ACGT]AGG
3827 CCTCAGG
HincII 599 GTCGAC GT[CT]^[GA]AC
3143 GTCGAC
HindII 599 GTCGAC GT[CT]^[GA]AC
3143 GTCGAC
KasI 526 GGCGCC G^GCGCC
1052 GGCGCC
MluNI 1969 TGGCCA TGG^CCA
3278 TGGCCA
MscI 1969 TGGCCA TGG^CCA
3278 TGGCCA
NarI 526 GGCGCC GG^CGCC
1052 GGCGCC
PflFI 635 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
976 GACCCAGTC
SalI 599 GTCGAC G^TCGAC
3143 GTCGAC
SfoI 526 GGCGCC GGC^GCC
1052 GGCGCC
Tth111I 635 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
976 GACCCAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII