##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AgeI AhdI Aor51HI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BbeI
BcgI BciVI BclI BfrI BlnI
BsaHI BsiWI BsmBI BsmI Bsp1407I
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Cfr10I DraI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII Hin1I HpaI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI SacII SapI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 1337 GTCGAC GT^[AC][GT]AC
AccII 986 CGCG CG^CG
AccIII 1250 TCCGGA T^CCGGA
AfaI 2283 GTAC GT^AC
Alw44I 649 GTGCAC G^TGCAC
AlwNI 743 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 649 GTGCAC G^TGCAC
AspI 1291 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 466 GGCACC G^G[CT][GA]CC
BbrPI 955 CACGTG CAC^GTG
BbsI 501 GAAGAC GAAGAC(2/6)
BglII 764 AGATCT A^GATCT
BlpI 1156 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1156 GCTGAGC GC^T[ACGT]AGC
BpuAI 501 GAAGAC GAAGAC(2/6)
BsaBI 1005 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1030 GAGACC GGTCTC(1/5)
BsaWI 1250 TCCGGA [AT]^CCGG[AT]
BseAI 1250 TCCGGA T^CCGGA
BsiEI 561 CGGTCG CG[GA][CT]^CG
BspDI 1675 ATCGAT AT^CGAT
BspEI 1250 TCCGGA T^CCGGA
BspHI 725 TCATGA T^CATGA
BspLU11I 2326 ACATGT A^CATGT
BsrDI 1746 CATTGC GCAATG(2/0)
BstUI 986 CGCG CG^CG
BstXI 1704 CCAGTGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1156 GCTGAGC GC^T[ACGT]AGC
ClaI 1675 ATCGAT AT^CGAT
CpoI 1252 CGGACCG CG^G[AT]CCG
EcoNI 582 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 2238 CCTTGG C^C[AT][AT]GG
HgaI 417 GACGC GACGC(5/10)
MamI 1005 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1250 TCCGGA T^CCGGA
MseI 1904 TTAA T^TAA
MslI 2 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 986 CGCG CG^CG
NspI 2326 ACATGT [GA]CATG^[CT]
PflFI 1291 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 955 CACGTG CAC^GTG
PmlI 955 CACGTG CAC^GTG
RcaI 725 TCATGA T^CATGA
RsaI 2283 GTAC GT^AC
RsrII 1252 CGGACCG CG^G[AT]CCG
SacI 2092 GAGCTC GAGCT^C
SalI 1337 GTCGAC G^TCGAC
SbfI 549 CCTGCAGG CCTGCA^GG
ScaI 2282 AGTACT AGT^ACT
SexAI 674 ACCAGGT A^CC[AT]GGT
Sse8387I 549 CCTGCAGG CCTGCA^GG
StuI 1602 AGGCCT AGG^CCT
StyI 2238 CCTTGG C^C[AT][AT]GG
Tru9I 1904 TTAA T^TAA
Tth111I 1291 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 2156 ACACGT A^C[GA][CT]GT
2326 ACATGT
AocI 1018 CCTCAGG CC^T[ACGT]AGG
2127 CCTGAGG
Asp700I 156 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
180 GAAAGCTTTC
AvaI 626 CCCGGG C^[CT]CG[GA]G
1479 CCCGGG
BalI 659 TGGCCA TGG^CCA
1697 TGGCCA
BanII 1987 GGGCTC G[GA]GC[CT]^C
2092 GAGCTC
BbvCI 1350 CCTCAGC CCTCAGC(-5/-2)
532 GCTGAGG
BglI 798 GCCTGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1771 GCCAGCTTGGC
BpmI 2335 CTCCAG CTGGAG(16/14)
808 CTGGAG
BsaAI 955 CACGTG [CT]AC^GT[GA]
2157 CACGTA
BsoBI 626 CCCGGG C^[CT]CG[GA]G
1479 CCCGGG
BspMI 552 GCAGGT ACCTGC(4/8)
1212 GCAGGT
Bsu36I 1018 CCTCAGG CC^T[ACGT]AGG
2127 CCTGAGG
CfoI 792 GCGC GCG^C
879 GCGC
DrdI 1240 GACATTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2155 GACACGTATGTC
EarI 2396 CTCTTC CTCTTC(1/4)
496 GAAGAG
Eco81I 1018 CCTCAGG CC^T[ACGT]AGG
2127 CCTGAGG
HhaI 792 GCGC GCG^C
879 GCGC
HinP1I 792 GCGC G^CGC
879 GCGC
HindIII 182 AAGCTT A^AGCTT
2292 AAGCTT
Ksp632I 2396 CTCTTC CTCTTC(1/4)
496 GAAGAG
MaeII 956 ACGT A^CGT
2158 ACGT
MluNI 659 TGGCCA TGG^CCA
1697 TGGCCA
MscI 659 TGGCCA TGG^CCA
1697 TGGCCA
SmaI 626 CCCGGG CCC^GGG
1479 CCCGGG
Tsp509I 2025 AATT ^AATT
2193 AATT
XcmI 1685 CCATGTTGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1743 CCACATTGCAGATGG
XmaCI 626 CCCGGG C^CCGGG
1479 CCCGGG
XmaI 626 CCCGGG C^CCGGG
1479 CCCGGG
XmnI 156 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
180 GAAAGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BbvI BcnI BfaI BmyI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DraIII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII