##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII BclI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BsrBI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
Eco47III EcoT22I FbaI FseI FspI
HpaI KpnI MfeI MluI MroI
MunI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 2268 GACGTC GACGT^C
AccI 2403 GTCGAC GT^[AC][GT]AC
AflIII 1264 ACATGT A^C[GA][CT]GT
Alw44I 1723 GTGCAC G^TGCAC
ApaLI 1723 GTGCAC G^TGCAC
Asp700I 4197 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3123 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2053 CCTAGG C^CTAGG
BbeI 90 GGCGCC GGCGC^C
BbrPI 659 CACGTG CAC^GTG
BbsI 2062 GAAGAC GAAGAC(2/6)
BciVI 705 GTATCC GTATCC(6/5)
BlnI 2053 CCTAGG C^CTAGG
BpuAI 2062 GAAGAC GAAGAC(2/6)
BsaBI 676 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3444 TCCGGT [AT]^CCGG[AT]
BsiEI 1739 CGGCCG CG[GA][CT]^CG
Bsp1407I 402 TGTACA T^GTACA
BspLU11I 1264 ACATGT A^CATGT
BsrGI 402 TGTACA T^GTACA
BssSI 1421 CACGAG CACGAG(-5/-1)
BstEII 787 GGTCACC G^GT[ACGT]ACC
BstPI 787 GGTCACC G^GT[ACGT]ACC
DraIII 3888 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1739 CGGCCG C^GGCCG
EarI 1905 CTCTTC CTCTTC(1/4)
EclXI 1739 CGGCCG C^GGCCG
Eco52I 1739 CGGCCG C^GGCCG
EcoO65I 787 GGTCACC G^GT[ACGT]ACC
EcoRI 192 GAATTC G^AATTC
KasI 90 GGCGCC G^GCGCC
Ksp632I 1905 CTCTTC CTCTTC(1/4)
MamI 676 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 742 GCCGGC GCC^GGC
NarI 90 GGCGCC GG^CGCC
NcoI 840 CCATGG C^CATGG
NdeI 1391 CATATG CA^TATG
NgoMIV 742 GCCGGC G^CCGGC
NheI 1748 GCTAGC G^CTAGC
PflFI 3123 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 659 CACGTG CAC^GTG
PmlI 659 CACGTG CAC^GTG
SalI 2403 GTCGAC G^TCGAC
SapI 1904 GCTCTTC GCTCTTC(1/4)
SfoI 90 GGCGCC GGC^GCC
SmaI 1898 CCCGGG CCC^GGG
SspBI 402 TGTACA T^GTACA
StuI 1553 AGGCCT AGG^CCT
Tth111I 3123 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1898 CCCGGG C^CCGGG
XmaI 1898 CCCGGG C^CCGGG
XmnI 4197 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaI 1898 CCCGGG C^[CT]CG[GA]G
3945 CCCGAG
BamHI 946 GGATCC G^GATCC
3039 GGATCC
BcgI 996 GCAAGGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3666 GCATGAGAATCG
BsaAI 659 CACGTG [CT]AC^GT[GA]
2487 TACGTG
BsoBI 1898 CCCGGG C^[CT]CG[GA]G
3945 CCCGAG
EcoNI 98 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
802 CCTGCCTCAGG
EcoRV 134 GATATC GAT^ATC
208 GATATC
HaeII 90 GGCGCC [GA]GCGC^[CT]
3979 GGCGCT
HgaI 69 GCGTC GACGC(5/10)
3969 GCGTC
HincII 2265 GTTGAC GT[CT]^[GA]AC
2403 GTCGAC
HindII 2265 GTTGAC GT[CT]^[GA]AC
2403 GTCGAC
HindIII 1441 AAGCTT A^AGCTT
2980 AAGCTT
KspI 64 CCGCGG CCGC^GG
2833 CCGCGG
SacII 64 CCGCGG CCGC^GG
2833 CCGCGG
XcmI 1311 CCACAAGCCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4089 CCAAGACTGCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BfrI BglI BglII BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII