##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AviII AvrII BciVI BlnI
BsiWI Bsp1407I BspLU11I BspMI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI FseI FspI
HincII HindII HpaI KpnI KspI
MfeI MluI MunI NdeI NheI
NotI NruI NspV PacI PinAI
PmeI Psp1406I PvuI SacII SalI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SspBI StuI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 958 GTAGAC GT^[AC][GT]AC
AfaI 1035 GTAC GT^AC
AflII 682 CTTAAG C^TTAAG
AhdI 2088 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3816 ATTAAT AT^TAAT
AsnI 3816 ATTAAT AT^TAAT
AspEI 2088 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 275 TGGCCA TGG^CCA
BamHI 2539 GGATCC G^GATCC
BbrPI 667 CACGTG CAC^GTG
BbvCI 3951 CCTCAGC CCTCAGC(-5/-2)
BclI 2048 TGATCA T^GATCA
BfrI 682 CTTAAG C^TTAAG
BglI 3054 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1725 AGATCT A^GATCT
BsaBI 1831 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3203 CGACCG CG[GA][CT]^CG
BspDI 3601 ATCGAT AT^CGAT
BspHI 1404 TCATGA T^CATGA
BsrFI 2870 GCCGGC [GA]^CCGG[CT]
BstAPI 784 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 4099 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2870 GCCGGC [GA]^CCGG[CT]
ClaI 3601 ATCGAT AT^CGAT
CpoI 3489 CGGTCCG CG^G[AT]CCG
DraIII 3303 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 258 GACGTTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 275 TGGCCA [CT]^GGCC[GA]
Eam1105I 2088 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 723 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 988 GATATC GAT^ATC
EcoT22I 402 ATGCAT ATGCA^T
FbaI 2048 TGATCA T^GATCA
MamI 1831 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 275 TGGCCA TGG^CCA
MscI 275 TGGCCA TGG^CCA
NaeI 2870 GCCGGC GCC^GGC
NgoMIV 2870 GCCGGC G^CCGGC
NsiI 402 ATGCAT ATGCA^T
NspI 299 GCATGC [GA]CATG^[CT]
PaeR7I 974 CTCGAG C^TCGAG
PflMI 735 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 667 CACGTG CAC^GTG
PmlI 667 CACGTG CAC^GTG
PshAI 2731 GACGACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3816 ATTAAT AT^TAAT
RcaI 1404 TCATGA T^CATGA
RsaI 1035 GTAC GT^AC
RsrII 3489 CGGTCCG CG^G[AT]CCG
SapI 625 GAAGAGC GCTCTTC(1/4)
SbfI 2403 CCTGCAGG CCTGCA^GG
SphI 299 GCATGC GCATG^C
Sse8387I 2403 CCTGCAGG CCTGCA^GG
Van91I 735 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2864 CCACGGGCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 974 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 2097 TCCGGA T^CCGGA
2542 TCCGGA
ApaI 3015 GGGCCC GGGCC^C
3021 GGGCCC
AspI 904 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3860 GACGGAGTC
BbeI 3161 GGCGCC GGCGC^C
3980 GGCGCC
BsaAI 667 CACGTG [CT]AC^GT[GA]
1475 TACGTG
BsaI 1448 GAGACC GGTCTC(1/5)
4057 GGTCTC
BsaWI 2097 TCCGGA [AT]^CCGG[AT]
2542 TCCGGA
BseAI 2097 TCCGGA T^CCGGA
2542 TCCGGA
BsgI 2131 CTGCAC GTGCAG(16/14)
3891 GTGCAG
BsmBI 3858 GAGACG CGTCTC(1/5)
4027 GAGACG
BspEI 2097 TCCGGA T^CCGGA
2542 TCCGGA
BsrBI 2967 CCGCTC CCGCTC(-3/-3)
3451 CCGCTC
BssHII 2850 GCGCGC G^CGCGC
3120 GCGCGC
BssSI 2517 CACGAG CACGAG(-5/-1)
4074 CTCGTG
BstYI 1725 AGATCT [GA]^GATC[CT]
2539 GGATCC
EarI 625 GAAGAG CTCTTC(1/4)
1495 GAAGAG
KasI 3161 GGCGCC G^GCGCC
3980 GGCGCC
Ksp632I 625 GAAGAG CTCTTC(1/4)
1495 GAAGAG
MflI 1725 AGATCT [GA]^GATC[CT]
2539 GGATCC
MroI 2097 TCCGGA T^CCGGA
2542 TCCGGA
NarI 3161 GGCGCC GG^CGCC
3980 GGCGCC
PflFI 904 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3860 GACGGAGTC
PvuII 736 CAGCTG CAG^CTG
898 CAGCTG
SacI 814 GAGCTC GAGCT^C
3482 GAGCTC
SfoI 3161 GGCGCC GGC^GCC
3980 GGCGCC
SspI 1146 AATATT AAT^ATT
1618 AATATT
Tth111I 904 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3860 GACGGAGTC
XhoII 1725 AGATCT [GA]^GATC[CT]
2539 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcgI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmaCI XmaI XmnI