##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
ApaI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BamHI
BbrPI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BsiEI BsiWI Bsp1407I
BspHI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII CpoI
DraIII EagI EclXI Eco52I EcoNI
EcoO65I FbaI FseI FspI HincII
HindII HpaI KpnI KspI MfeI
MluI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SfiI
SfuI SgrAI SnaBI SspBI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 577 TCCGGA T^CCGGA
AcyI 162 GGCGCC G[GA]^CG[CT]C
AflIII 651 ACATGT A^C[GA][CT]GT
AhdI 3268 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2389 GTGCAC G^TGCAC
Aor51HI 1035 AGCGCT AGC^GCT
ApaLI 2389 GTGCAC G^TGCAC
AspEI 3268 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 162 GGCGCC GGCGC^C
BcgI 3220 CGAGTGTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2722 GGATAC GTATCC(6/5)
BglI 1859 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2099 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 162 GGCGCC G[GA]^CG[CT]C
BsaWI 577 TCCGGA [AT]^CCGG[AT]
BseAI 577 TCCGGA T^CCGGA
BsmI 2183 GAATGC GAATGC(1/-1)
BspDI 3981 ATCGAT AT^CGAT
BspEI 577 TCCGGA T^CCGGA
BspLU11I 651 ACATGT A^CATGT
BsrBI 2691 GAGCGG CCGCTC(-3/-3)
BsrDI 980 CATTGC GCAATG(2/0)
ClaI 3981 ATCGAT AT^CGAT
DraI 3234 TTTAAA TTT^AAA
DrdI 1658 GACAGCTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 86 CCACGG C^C[GA][CT]GG
Eam1105I 3268 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1035 AGCGCT AGC^GCT
EcoRI 1799 GAATTC G^AATTC
EcoT14I 3010 CCAAGG C^C[AT][AT]GG
Hin1I 162 GGCGCC G[GA]^CG[CT]C
KasI 162 GGCGCC G^GCGCC
MamI 2099 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 577 TCCGGA T^CCGGA
NarI 162 GGCGCC GG^CGCC
PacI 729 TTAATTAA TTAAT^TAA
PshAI 1178 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2286 GAGCTC GAGCT^C
SfoI 162 GGCGCC GGC^GCC
SphI 2158 GCATGC GCATG^C
StyI 3010 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AocI 2766 CCTCAGG CC^T[ACGT]AGG
4073 CCTGAGG
BalI 1163 TGGCCA TGG^CCA
1619 TGGCCA
BanII 1448 GAGCCC G[GA]GC[CT]^C
2286 GAGCTC
BsmBI 144 GAGACG CGTCTC(1/5)
4033 GAGACG
BssHII 25 GCGCGC G^CGCGC
27 GCGCGC
BstAPI 2382 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2929 GCATCCACTGC
Bsu36I 2766 CCTCAGG CC^T[ACGT]AGG
4073 CCTGAGG
EarI 3289 CTCTTC CTCTTC(1/4)
2460 GAAGAG
Eco81I 2766 CCTCAGG CC^T[ACGT]AGG
4073 CCTGAGG
EcoRV 385 GATATC GAT^ATC
2526 GATATC
EcoT22I 2484 ATGCAT ATGCA^T
3090 ATGCAT
HaeII 162 GGCGCC [GA]GCGC^[CT]
1035 AGCGCT
HindIII 921 AAGCTT A^AGCTT
2682 AAGCTT
Ksp632I 3289 CTCTTC CTCTTC(1/4)
2460 GAAGAG
MaeII 2302 ACGT A^CGT
4036 ACGT
MluNI 1163 TGGCCA TGG^CCA
1619 TGGCCA
MscI 1163 TGGCCA TGG^CCA
1619 TGGCCA
NsiI 2484 ATGCAT ATGCA^T
3090 ATGCAT
SapI 2460 GAAGAGC GCTCTTC(1/4)
3288 GCTCTTC
SmaI 2784 CCCGGG CCC^GGG
3200 CCCGGG
SmlI 1304 CTTGAG C^T[CT][GA]AG
2364 CTTGAG
SpeI 1344 ACTAGT A^CTAGT
3871 ACTAGT
SspI 603 AATATT AAT^ATT
826 AATATT
XcmI 1121 CCAGGGTGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2394 CCATTTCTAAAGTGG
XmaCI 2784 CCCGGG C^CCGGG
3200 CCCGGG
XmaI 2784 CCCGGG C^CCGGG
3200 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BbsI BbvCI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
SbfI ScrFI SexAI SfaNI SfcI
Sse8387I StuI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII