##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII Acc65I AccIII AflII AhdI
AscI AseI AsnI Asp718I AspEI
AspI AviII BbrPI BfrI BseAI
BsiWI BspDI BspEI BspHI BstBI
BstXI ClaI CpoI Eam1105I FseI
FspI KpnI MroI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshBI
PvuI RcaI RsrII SexAI SfiI
SfuI SgrAI SnaBI SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AclI 106 AACGTT AA^CGTT
AgeI 1982 ACCGGT A^CCGGT
AocI 1508 CCTCAGG CC^T[ACGT]AGG
AvrII 5961 CCTAGG C^CTAGG
BamHI 2986 GGATCC G^GATCC
BbsI 496 GAAGAC GAAGAC(2/6)
BbvCI 5487 GCTGAGG CCTCAGC(-5/-2)
BcgI 6650 GCACAGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 5961 CCTAGG C^CTAGG
BpuAI 496 GAAGAC GAAGAC(2/6)
BsaBI 126 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 3732 GAATGC GAATGC(1/-1)
BsrBI 1284 GAGCGG CCGCTC(-3/-3)
Bst1107I 6115 GTATAC GTA^TAC
BstEII 2620 GGTAACC G^GT[ACGT]ACC
BstPI 2620 GGTAACC G^GT[ACGT]ACC
BstZ17I 6115 GTATAC GTA^TAC
Bsu36I 1508 CCTCAGG CC^T[ACGT]AGG
Eco81I 1508 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2620 GGTAACC G^GT[ACGT]ACC
EcoRV 4221 GATATC GAT^ATC
HindIII 706 AAGCTT A^AGCTT
MamI 126 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 4547 ACGCGT A^CGCGT
NaeI 5074 GCCGGC GCC^GGC
NgoMIV 5074 GCCGGC G^CCGGC
PinAI 1982 ACCGGT A^CCGGT
Psp1406I 106 AACGTT AA^CGTT
SalI 3458 GTCGAC G^TCGAC
SpeI 2932 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 3458 GTCGAC GT^[AC][GT]AC
6115 GTATAC
Aor51HI 1394 AGCGCT AGC^GCT
6654 AGCGCT
ApaI 4534 GGGCCC GGGCC^C
5005 GGGCCC
BciVI 3355 GGATAC GTATCC(6/5)
5401 GGATAC
BglII 1412 AGATCT A^GATCT
3341 AGATCT
BsaAI 3295 TACGTG [CT]AC^GT[GA]
6534 CACGTA
BsaWI 1831 ACCGGA [AT]^CCGG[AT]
1982 ACCGGT
BsmBI 393 CGTCTC CGTCTC(1/5)
3995 CGTCTC
BspLU11I 1716 ACATGT A^CATGT
6188 ACATGT
DrdI 466 GACAGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6782 GACCACCTTGTC
Eco47III 1394 AGCGCT AGC^GCT
6654 AGCGCT
EcoRI 2394 GAATTC G^AATTC
5318 GAATTC
EcoT22I 952 ATGCAT ATGCA^T
2012 ATGCAT
HpaI 729 GTTAAC GTT^AAC
1447 GTTAAC
MfeI 1175 CAATTG C^AATTG
6221 CAATTG
MunI 1175 CAATTG C^AATTG
6221 CAATTG
NsiI 952 ATGCAT ATGCA^T
2012 ATGCAT
PshAI 3026 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4617 GACCCCCGTC
SapI 1105 GAAGAGC GCTCTTC(1/4)
1575 GCTCTTC
SbfI 1670 CCTGCAGG CCTGCA^GG
5517 CCTGCAGG
ScaI 887 AGTACT AGT^ACT
4748 AGTACT
Sse8387I 1670 CCTGCAGG CCTGCA^GG
5517 CCTGCAGG
XbaI 3093 TCTAGA T^CTAGA
6270 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 154 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvI BclI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrDI
BsrFI BsrGI BsrI BssHII BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
SphI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI