##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
Acc65I AclI Aor51HI AscI Asp718I
AviII AvrII BbeI BbrPI BlnI
BsaBI BsiWI BspDI BssHII Bst1107I
BstZ17I ClaI CpoI DrdI Eco47III
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NarI NheI
NruI NsiI PacI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SbfI ScaI SfoI SgrAI
Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 826 GACGTC GACGT^C
AccIII 5465 TCCGGA T^CCGGA
AflII 2698 CTTAAG C^TTAAG
AgeI 6542 ACCGGT A^CCGGT
Alw44I 1734 GTGCAC G^TGCAC
AocI 387 CCTGAGG CC^T[ACGT]AGG
ApaLI 1734 GTGCAC G^TGCAC
AseI 1234 ATTAAT AT^TAAT
AsnI 1234 ATTAAT AT^TAAT
BamHI 1442 GGATCC G^GATCC
BciVI 1379 GGATAC GTATCC(6/5)
BfrI 2698 CTTAAG C^TTAAG
BsaAI 420 TACGTA [CT]AC^GT[GA]
BseAI 5465 TCCGGA T^CCGGA
BspEI 5465 TCCGGA T^CCGGA
BspHI 2321 TCATGA T^CATGA
BspLU11I 494 ACATGT A^CATGT
BstBI 4801 TTCGAA TT^CGAA
BstEII 6538 GGTAACC G^GT[ACGT]ACC
BstPI 6538 GGTAACC G^GT[ACGT]ACC
Bsu36I 387 CCTGAGG CC^T[ACGT]AGG
DraI 6222 TTTAAA TTT^AAA
DraIII 6717 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 4310 CGGCCG C^GGCCG
EclXI 4310 CGGCCG C^GGCCG
Eco52I 4310 CGGCCG C^GGCCG
Eco81I 387 CCTGAGG CC^T[ACGT]AGG
EcoO65I 6538 GGTAACC G^GT[ACGT]ACC
EcoRI 6669 GAATTC G^AATTC
MroI 5465 TCCGGA T^CCGGA
NdeI 1358 CATATG CA^TATG
NotI 4309 GCGGCCGC GC^GGCCGC
NspV 4801 TTCGAA TT^CGAA
PinAI 6542 ACCGGT A^CCGGT
PshBI 1234 ATTAAT AT^TAAT
RcaI 2321 TCATGA T^CATGA
SalI 490 GTCGAC G^TCGAC
SfiI 660 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 4801 TTCGAA TT^CGAA
SnaBI 420 TACGTA TAC^GTA
SpeI 35 ACTAGT A^CTAGT
SphI 566 GCATGC GCATG^C
XbaI 5890 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 490 GTCGAC GT^[AC][GT]AC
4077 GTCTAC
AflIII 494 ACATGT A^C[GA][CT]GT
4073 ACGTGT
AhdI 1177 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4000 GACGTGGCGTC
AspEI 1177 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4000 GACGTGGCGTC
BanI 70 GGCACC G^G[CT][GA]CC
6595 GGTGCC
BcgI 6588 CGAAATGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
815 GCATCAACTTCG
BlpI 5379 GCTCAGC GC^T[ACGT]AGC
6277 GCTGAGC
Bpu1102I 5379 GCTCAGC GC^T[ACGT]AGC
6277 GCTGAGC
Bsp1407I 497 TGTACA T^GTACA
2042 TGTACA
BsrGI 497 TGTACA T^GTACA
2042 TGTACA
CelII 5379 GCTCAGC GC^T[ACGT]AGC
6277 GCTGAGC
Eam1105I 1177 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4000 GACGTGGCGTC
NaeI 2207 GCCGGC GCC^GGC
6601 GCCGGC
NgoMIV 2207 GCCGGC G^CCGGC
6601 GCCGGC
SexAI 4367 ACCTGGT A^CC[AT]GGT
4619 ACCTGGT
SmaI 3896 CCCGGG CCC^GGG
4820 CCCGGG
SspBI 497 TGTACA T^GTACA
2042 TGTACA
XmaCI 3896 CCCGGG C^CCGGG
4820 CCCGGG
XmaI 3896 CCCGGG C^CCGGG
4820 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BanII BbsI BbvCI BbvI
BclI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PaeR7I PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoI XhoII XmnI