##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AscI AseI AsnI Asp700I AviII
AvrII BbrPI BcgI BfrI BlnI
BsaBI BseAI BsiWI Bsp1407I BspEI
BsrGI BssHII Bst1107I BstEII BstPI
BstZ17I CpoI DrdI EcoO65I EcoRV
FseI FspI HindIII KspI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SexAI SnaBI SpeI SspBI
SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 740 GACGTC GACGT^C
Acc65I 3008 GGTACC G^GTACC
AccII 122 CGCG CG^CG
Alw44I 588 GTGCAC G^TGCAC
Aor51HI 4 AGCGCT AGC^GCT
ApaLI 588 GTGCAC G^TGCAC
Asp718I 3008 GGTACC G^GTACC
AspI 4326 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2474 GGATCC G^GATCC
BbvCI 3045 GCTGAGG CCTCAGC(-5/-2)
BciVI 603 GTATCC GTATCC(6/5)
BsaWI 1555 ACCGGA [AT]^CCGG[AT]
BsiEI 3000 CGGCCG CG[GA][CT]^CG
BsmBI 905 CGTCTC CGTCTC(1/5)
BspDI 2077 ATCGAT AT^CGAT
BspHI 736 TCATGA T^CATGA
BsrBI 2070 GAGCGG CCGCTC(-3/-3)
BstAPI 1755 GCATGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3989 TTCGAA TT^CGAA
BstUI 122 CGCG CG^CG
ClaI 2077 ATCGAT AT^CGAT
EagI 3000 CGGCCG C^GGCCG
EclXI 3000 CGGCCG C^GGCCG
Eco47III 4 AGCGCT AGC^GCT
Eco52I 3000 CGGCCG C^GGCCG
EcoRI 3497 GAATTC G^AATTC
HpaI 578 GTTAAC GTT^AAC
KpnI 3008 GGTACC GGTAC^C
MvnI 122 CGCG CG^CG
NaeI 1851 GCCGGC GCC^GGC
NgoMIV 1851 GCCGGC G^CCGGC
NspV 3989 TTCGAA TT^CGAA
PflFI 4326 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 736 TCATGA T^CATGA
SfiI 2047 GGCCTGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3989 TTCGAA TT^CGAA
SgrAI 2063 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 3445 CCCGGG CCC^GGG
SmlI 4284 CTTGAG C^T[CT][GA]AG
SphI 2155 GCATGC GCATG^C
Tth111I 4326 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3971 TCTAGA T^CTAGA
XmaCI 3445 CCCGGG C^CCGGG
XmaI 3445 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 3406 GAATTT [GA]^AATT[CT]
3497 GAATTC
AhdI 2632 GACTCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3756 GACTTAATGTC
AocI 1601 CCTCAGG CC^T[ACGT]AGG
2112 CCTGAGG
ApaI 999 GGGCCC GGGCC^C
3552 GGGCCC
ApoI 3406 GAATTT [GA]^AATT[CT]
3497 GAATTC
AspEI 2632 GACTCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3756 GACTTAATGTC
BalI 3920 TGGCCA TGG^CCA
4090 TGGCCA
BbeI 1402 GGCGCC GGCGC^C
2061 GGCGCC
BbsI 1253 GAAGAC GAAGAC(2/6)
264 GTCTTC
BglII 869 AGATCT A^GATCT
2853 AGATCT
BpuAI 1253 GAAGAC GAAGAC(2/6)
264 GTCTTC
BsaAI 1440 TACGTG [CT]AC^GT[GA]
3338 TACGTG
BsaI 1769 GAGACC GGTCTC(1/5)
4301 GAGACC
BsmI 686 GAATGC GAATGC(1/-1)
3026 GAATGC
BspLU11I 1426 ACATGT A^CATGT
2339 ACATGT
BspMI 956 GCAGGT ACCTGC(4/8)
1893 GCAGGT
BssSI 884 CACGAG CACGAG(-5/-1)
3016 CTCGTG
Bsu36I 1601 CCTCAGG CC^T[ACGT]AGG
2112 CCTGAGG
DraI 3034 TTTAAA TTT^AAA
3692 TTTAAA
Eam1105I 2632 GACTCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3756 GACTTAATGTC
Eco81I 1601 CCTCAGG CC^T[ACGT]AGG
2112 CCTGAGG
KasI 1402 GGCGCC G^GCGCC
2061 GGCGCC
MluNI 3920 TGGCCA TGG^CCA
4090 TGGCCA
MscI 3920 TGGCCA TGG^CCA
4090 TGGCCA
NarI 1402 GGCGCC GG^CGCC
2061 GGCGCC
PflMI 2180 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4113 CCACAGGATGG
SacI 1338 GAGCTC GAGCT^C
4287 GAGCTC
SapI 914 GCTCTTC GCTCTTC(1/4)
2934 GCTCTTC
SbfI 3116 CCTGCAGG CCTGCA^GG
3221 CCTGCAGG
SfoI 1402 GGCGCC GGC^GCC
2061 GGCGCC
Sse8387I 3116 CCTGCAGG CCTGCA^GG
3221 CCTGCAGG
Van91I 2180 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4113 CCACAGGATGG
XcmI 1531 CCACCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2851 CCAGATCTGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvI BclI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PshAI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII