##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AclI AgeI Aor51HI ApaI AscI
Asp700I AspI AviII AvrII BbrPI
BcgI BclI BlnI BsiEI BsiWI
BsmBI BsmI BspDI BspHI BspLU11I
BsrDI BssHII Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
KspI MfeI MluI MunI NcoI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacII ScaI
SexAI SfiI SgrAI SnaBI SpeI
Tth111I Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 1971 GGTACC G^GTACC
AccI 1657 GTCGAC GT^[AC][GT]AC
AccII 1424 CGCG CG^CG
AflII 1558 CTTAAG C^TTAAG
Alw44I 2546 GTGCAC G^TGCAC
ApaLI 2546 GTGCAC G^TGCAC
AseI 1360 ATTAAT AT^TAAT
AsnI 1360 ATTAAT AT^TAAT
Asp718I 1971 GGTACC G^GTACC
BamHI 647 GGATCC G^GATCC
BbeI 1613 GGCGCC GGCGC^C
BbvCI 2458 GCTGAGG CCTCAGC(-5/-2)
BciVI 1523 GTATCC GTATCC(6/5)
BfrI 1558 CTTAAG C^TTAAG
BglI 1605 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3271 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3271 GCTAAGC GC^T[ACGT]AGC
BsaAI 2141 TACGTG [CT]AC^GT[GA]
BssSI 833 CACGAG CACGAG(-5/-1)
BstBI 2205 TTCGAA TT^CGAA
BstUI 1424 CGCG CG^CG
BstXI 1153 CCAGGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3271 GCTAAGC GC^T[ACGT]AGC
DraI 2954 TTTAAA TTT^AAA
EcoRI 1311 GAATTC G^AATTC
HpaI 3128 GTTAAC GTT^AAC
KasI 1613 GGCGCC G^GCGCC
KpnI 1971 GGTACC GGTAC^C
MvnI 1424 CGCG CG^CG
NaeI 2148 GCCGGC GCC^GGC
NarI 1613 GGCGCC GG^CGCC
NgoMIV 2148 GCCGGC G^CCGGC
NspI 615 GCATGC [GA]CATG^[CT]
NspV 2205 TTCGAA TT^CGAA
PshBI 1360 ATTAAT AT^TAAT
SalI 1657 GTCGAC G^TCGAC
SapI 2985 GCTCTTC GCTCTTC(1/4)
SfoI 1613 GGCGCC GGC^GCC
SfuI 2205 TTCGAA TT^CGAA
SmaI 480 CCCGGG CCC^GGG
SphI 615 GCATGC GCATG^C
StuI 312 AGGCCT AGG^CCT
SwaI 2953 ATTTAAAT ATTT^AAAT
XmaCI 480 CCCGGG C^CCGGG
XmaI 480 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 2660 GACGTC GACGT^C
3089 GACGTC
AccIII 1467 TCCGGA T^CCGGA
2232 TCCGGA
AcsI 1311 GAATTC [GA]^AATT[CT]
2951 AAATTT
AflIII 2142 ACGTGT A^C[GA][CT]GT
3216 ACGTGT
ApoI 1311 GAATTC [GA]^AATT[CT]
2951 AAATTT
AvaI 187 CTCGGG C^[CT]CG[GA]G
480 CCCGGG
BalI 294 TGGCCA TGG^CCA
846 TGGCCA
BglII 37 AGATCT A^GATCT
2352 AGATCT
BsaBI 352 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2632 GATGAATATC
BseAI 1467 TCCGGA T^CCGGA
2232 TCCGGA
BsgI 584 CTGCAC GTGCAG(16/14)
1241 GTGCAG
BsoBI 187 CTCGGG C^[CT]CG[GA]G
480 CCCGGG
Bsp1407I 3019 TGTACA T^GTACA
3140 TGTACA
BspEI 1467 TCCGGA T^CCGGA
2232 TCCGGA
BsrGI 3019 TGTACA T^GTACA
3140 TGTACA
DsaI 2401 CCACGG C^C[GA][CT]GG
3194 CCACGG
HincII 1657 GTCGAC GT[CT]^[GA]AC
3128 GTTAAC
HindII 1657 GTCGAC GT[CT]^[GA]AC
3128 GTTAAC
HindIII 193 AAGCTT A^AGCTT
299 AAGCTT
MamI 352 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2632 GATGAATATC
MluNI 294 TGGCCA TGG^CCA
846 TGGCCA
MroI 1467 TCCGGA T^CCGGA
2232 TCCGGA
MscI 294 TGGCCA TGG^CCA
846 TGGCCA
SspBI 3019 TGTACA T^GTACA
3140 TGTACA
SspI 1357 AATATT AAT^ATT
3145 AATATT
XcmI 564 CCACCCGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3382 CCATCAATAAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcyI AfaI AhdI AluI
AlwI AlwNI AocI AspEI AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI Eam1105I
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI Sse8387I StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII