##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AvaI
AviII BamHI BanI BbeI BbrPI
BbvCI BcnI BglI BsaAI BsaWI
BseAI BsiWI BsoBI BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I FseI FspI
HaeII HapII HhaI HinP1I HpaI
HpaII KasI KpnI KspI MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AseI 1048 ATTAAT AT^TAAT
AsnI 1048 ATTAAT AT^TAAT
BalI 762 TGGCCA TGG^CCA
BanII 1743 GAGCTC G[GA]GC[CT]^C
BciVI 2809 GTATCC GTATCC(6/5)
BglII 991 AGATCT A^GATCT
BlpI 213 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 213 GCTCAGC GC^T[ACGT]AGC
BsaBI 1630 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 608 GAGACC GGTCTC(1/5)
BsiEI 558 CGACCG CG[GA][CT]^CG
BsmBI 1296 GAGACG CGTCTC(1/5)
BsmI 1360 GAATGC GAATGC(1/-1)
Bsp1407I 2263 TGTACA T^GTACA
BsrDI 2357 CATTGC GCAATG(2/0)
BsrGI 2263 TGTACA T^GTACA
BstEII 2632 GGTAACC G^GT[ACGT]ACC
BstPI 2632 GGTAACC G^GT[ACGT]ACC
BstXI 3460 CCAACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 213 GCTCAGC GC^T[ACGT]AGC
DraIII 1560 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 762 TGGCCA [CT]^GGCC[GA]
EcoO65I 2632 GGTAACC G^GT[ACGT]ACC
EcoRI 1112 GAATTC G^AATTC
EcoT22I 1395 ATGCAT ATGCA^T
FauI 194 GCGGG CCCGC(4/6)
MaeII 1966 ACGT A^CGT
MamI 1630 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 762 TGGCCA TGG^CCA
MscI 762 TGGCCA TGG^CCA
NdeI 3448 CATATG CA^TATG
NsiI 1395 ATGCAT ATGCA^T
PshBI 1048 ATTAAT AT^TAAT
SacI 1743 GAGCTC GAGCT^C
SapI 1026 GAAGAGC GCTCTTC(1/4)
SphI 3651 GCATGC GCATG^C
SspBI 2263 TGTACA T^GTACA
SspI 3073 AATATT AAT^ATT
TaqI 151 TCGA T^CGA
TthHB8I 151 TCGA T^CGA
XbaI 934 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 550 GACGCC G[GA]^CG[CT]C
2659 GACGCC
AflII 1004 CTTAAG C^TTAAG
2906 CTTAAG
AlwNI 14 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
1724 CAGCAGCTG
AvrII 2245 CCTAGG C^CTAGG
3903 CCTAGG
BcgI 558 CGACCGATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1120 CGAACCCAATGC
BclI 1086 TGATCA T^GATCA
3610 TGATCA
BfrI 1004 CTTAAG C^TTAAG
2906 CTTAAG
BlnI 2245 CCTAGG C^CTAGG
3903 CCTAGG
BsaHI 550 GACGCC G[GA]^CG[CT]C
2659 GACGCC
BspHI 753 TCATGA T^CATGA
1134 TCATGA
DsaI 603 CCATGG C^C[GA][CT]GG
639 CCATGG
EcoNI 3213 CCTTCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3903 CCTAGGTGAGG
EcoRV 1979 GATATC GAT^ATC
3060 GATATC
FbaI 1086 TGATCA T^GATCA
3610 TGATCA
HgaI 550 GACGC GACGC(5/10)
2659 GACGC
Hin1I 550 GACGCC G[GA]^CG[CT]C
2659 GACGCC
HincII 2062 GTTGAC GT[CT]^[GA]AC
2206 GTTGAC
HindII 2062 GTTGAC GT[CT]^[GA]AC
2206 GTTGAC
NcoI 603 CCATGG C^CATGG
639 CCATGG
NspI 3651 GCATGC [GA]CATG^[CT]
3702 ACATGC
RcaI 753 TCATGA T^CATGA
1134 TCATGA
ScaI 2485 AGTACT AGT^ACT
4180 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII