##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
BbrPI BciVI BfrI BglI BseAI
BsiWI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII Eco47III Eco81I EcoRI
EcoT22I FseI HindIII KpnI MfeI
MluI MroI MunI NaeI NcoI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AcyI 3499 GGCGCC G[GA]^CG[CT]C
AhdI 279 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 283 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 279 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2402 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1347 TGCGCA TGC^GCA
AvrII 2435 CCTAGG C^CTAGG
BalI 3140 TGGCCA TGG^CCA
BamHI 2679 GGATCC G^GATCC
BbeI 3499 GGCGCC GGCGC^C
BbvCI 3324 CCTCAGC CCTCAGC(-5/-2)
BcgI 3313 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1410 AGATCT A^GATCT
BlnI 2435 CCTAGG C^CTAGG
BlpI 3753 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3753 GCTCAGC GC^T[ACGT]AGC
BsaAI 1484 TACGTG [CT]AC^GT[GA]
BsaHI 3499 GGCGCC G[GA]^CG[CT]C
BsgI 3262 GTGCAG GTGCAG(16/14)
BsiEI 756 CGGCCG CG[GA][CT]^CG
BsmBI 2258 GAGACG CGTCTC(1/5)
BspHI 2977 TCATGA T^CATGA
BsrDI 3899 GCAATG GCAATG(2/0)
BstXI 3467 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3753 GCTCAGC GC^T[ACGT]AGC
EagI 756 CGGCCG C^GGCCG
Eam1105I 279 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 756 CGGCCG C^GGCCG
Eco52I 756 CGGCCG C^GGCCG
EcoNI 2493 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1347 TGCGCA TGC^GCA
HaeII 3499 GGCGCC [GA]GCGC^[CT]
HgaI 2402 GACGC GACGC(5/10)
Hin1I 3499 GGCGCC G[GA]^CG[CT]C
KasI 3499 GGCGCC G^GCGCC
KspI 3104 CCGCGG CCGC^GG
MluNI 3140 TGGCCA TGG^CCA
MscI 3140 TGGCCA TGG^CCA
NarI 3499 GGCGCC GG^CGCC
NdeI 1440 CATATG CA^TATG
PflFI 2402 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2977 TCATGA T^CATGA
SacII 3104 CCGCGG CCGC^GG
SbfI 692 CCTGCAGG CCTGCA^GG
SfoI 3499 GGCGCC GGC^GCC
SmaI 2759 CCCGGG CCC^GGG
Sse8387I 692 CCTGCAGG CCTGCA^GG
SwaI 2184 ATTTAAAT ATTT^AAAT
Tth111I 2402 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2759 CCCGGG C^CCGGG
XmaI 2759 CCCGGG C^CCGGG
XmnI 283 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 2399 GTAGAC GT^[AC][GT]AC
3672 GTAGAC
AlwNI 1532 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
1968 CAGCATCTG
BbsI 547 GAAGAC GAAGAC(2/6)
287 GTCTTC
BclI 1082 TGATCA T^GATCA
3809 TGATCA
BpuAI 547 GAAGAC GAAGAC(2/6)
287 GTCTTC
BsaI 688 GAGACC GGTCTC(1/5)
3425 GGTCTC
BseRI 2752 CTCCTC GAGGAG(10/8)
1138 GAGGAG
BssSI 367 CTCGTG CACGAG(-5/-1)
3442 CTCGTG
BstEII 2264 GGTTACC G^GT[ACGT]ACC
2763 GGTCACC
BstPI 2264 GGTTACC G^GT[ACGT]ACC
2763 GGTCACC
DsaI 3104 CCGCGG C^C[GA][CT]GG
3495 CCACGG
EaeI 756 CGGCCG [CT]^GGCC[GA]
3140 TGGCCA
EcoO65I 2264 GGTTACC G^GT[ACGT]ACC
2763 GGTCACC
EcoRV 442 GATATC GAT^ATC
799 GATATC
FbaI 1082 TGATCA T^GATCA
3809 TGATCA
HpaI 1579 GTTAAC GTT^AAC
2459 GTTAAC
NheI 1275 GCTAGC G^CTAGC
2888 GCTAGC
PpuMI 650 GGGTCCT [GA]G^G[AT]CC[CT]
899 AGGTCCT
PvuII 1270 CAGCTG CAG^CTG
2454 CAGCTG
ScaI 1394 AGTACT AGT^ACT
3592 AGTACT
SspI 1354 AATATT AAT^ATT
2816 AATATT
StuI 1876 AGGCCT AGG^CCT
2428 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaBI BsaJI
BsaWI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspLU11I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DrdI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII