##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Alw44I ApaI ApaLI AscI
AvaI BanII BbeI BcgI BclI
BcnI BglII BlpI Bpu1102I BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspEI BsrBI BsrFI
BssHII BssSI BstAPI BstEII BstPI
BstUI CelII Cfr10I CpoI DraII
DraIII DrdI EagI EclXI Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI FseI HgaI Hin1I
KasI KspI MluI MroI MspA1I
MvnI NaeI NarI NciI NcoI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmeI PpuMI PshAI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
StuI SwaI XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 74 enzymes) #
###################################
Acc65I 4035 GGTACC G^GTACC
AciI 1656 GCGG CCGC(-3/-1)
AflII 1259 CTTAAG C^TTAAG
AhdI 1518 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 118 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 4342 CCTAAGG CC^T[ACGT]AGG
AseI 2361 ATTAAT AT^TAAT
AsnI 2361 ATTAAT AT^TAAT
Asp700I 3339 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4035 GGTACC G^GTACC
AspEI 1518 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 52 GTGCTC G[AT]GC[AT]^C
AspI 2691 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 2447 GGACC G^G[AT]CC
AviII 3518 TGCGCA TGC^GCA
AvrII 269 CCTAGG C^CTAGG
BalI 464 TGGCCA TGG^CCA
BamHI 59 GGATCC G^GATCC
BanI 4035 GGTACC G^G[CT][GA]CC
BbrPI 988 CACGTG CAC^GTG
BbvCI 996 CCTCAGC CCTCAGC(-5/-2)
BfrI 1259 CTTAAG C^TTAAG
BglI 3642 GCCAGAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 269 CCTAGG C^CTAGG
BmyI 52 GTGCTC G[AGT]GC[ACT]^C
BsaAI 988 CACGTG [CT]AC^GT[GA]
BseRI 2646 CTCCTC GAGGAG(10/8)
BsgI 2341 GTGCAG GTGCAG(16/14)
BsiHKAI 52 GTGCTC G[AT]GC[AT]^C
BsiYI 3415 CCAGCTAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 3415 CCAGCTAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 584 GAATGC GAATGC(1/-1)
Bsp1286I 52 GTGCTC G[AGT]GC[ACT]^C
BspDI 128 ATCGAT AT^CGAT
BspHI 4318 TCATGA T^CATGA
BspMI 2517 GCAGGT ACCTGC(4/8)
BsrDI 2895 CATTGC GCAATG(2/0)
Bst1107I 1500 GTATAC GTA^TAC
BstBI 3662 TTCGAA TT^CGAA
BstZ17I 1500 GTATAC GTA^TAC
Bsu36I 4342 CCTAAGG CC^T[ACGT]AGG
ClaI 128 ATCGAT AT^CGAT
DsaI 969 CCACGG C^C[GA][CT]GG
EaeI 464 TGGCCA [CT]^GGCC[GA]
Eam1105I 1518 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 4307 CTCTTC CTCTTC(1/4)
Eco81I 4342 CCTAAGG CC^T[ACGT]AGG
EcoT14I 269 CCTAGG C^C[AT][AT]GG
FspI 3518 TGCGCA TGC^GCA
HapII 63 CCGG C^CGG
HpaI 1776 GTTAAC GTT^AAC
HpaII 63 CCGG C^CGG
KpnI 4035 GGTACC GGTAC^C
Ksp632I 4307 CTCTTC CTCTTC(1/4)
MfeI 1904 CAATTG C^AATTG
MluNI 464 TGGCCA TGG^CCA
MscI 464 TGGCCA TGG^CCA
MspI 63 CCGG C^CGG
MunI 1904 CAATTG C^AATTG
NheI 873 GCTAGC G^CTAGC
NspV 3662 TTCGAA TT^CGAA
PflFI 2691 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3415 CCAGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 988 CACGTG CAC^GTG
PmlI 988 CACGTG CAC^GTG
PshBI 2361 ATTAAT AT^TAAT
PstI 2616 CTGCAG CTGCA^G
RcaI 4318 TCATGA T^CATGA
SfcI 2616 CTGCAG C^T[GA][CT]AG
SfuI 3662 TTCGAA TT^CGAA
StyI 269 CCTAGG C^C[AT][AT]GG
Tth111I 2691 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3415 CCAGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3339 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 1500 GTATAC GT^[AC][GT]AC
2654 GTAGAC
Aor51HI 2100 AGCGCT AGC^GCT
2116 AGCGCT
BbsI 455 GTCTTC GAAGAC(2/6)
2307 GTCTTC
BciVI 3800 GGATAC GTATCC(6/5)
1395 GTATCC
BpuAI 455 GTCTTC GAAGAC(2/6)
2307 GTCTTC
BsaBI 945 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2346 GATGTAAATC
BsmFI 300 GTCCC GGGAC(10/14)
1015 GTCCC
BspLU11I 2399 ACATGT A^CATGT
3534 ACATGT
BstXI 2450 CCAAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3901 CCAGAGGTCTGG
Eco47III 2100 AGCGCT AGC^GCT
2116 AGCGCT
EcoT22I 430 ATGCAT ATGCA^T
738 ATGCAT
HaeII 2100 AGCGCT [GA]GCGC^[CT]
2116 AGCGCT
HincII 722 GTCAAC GT[CT]^[GA]AC
1776 GTTAAC
HindII 722 GTCAAC GT[CT]^[GA]AC
1776 GTTAAC
MamI 945 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2346 GATGTAAATC
NdeI 141 CATATG CA^TATG
154 CATATG
NsiI 430 ATGCAT ATGCA^T
738 ATGCAT
Tsp45I 986 GTCAC ^GT[GC]AC
2662 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI BbvI BfaI BpmI BsaJI
BsmAI Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HindIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SmlI SpeI SphI SspBI
SspI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII