##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AviII
AvrII BbeI BcgI BclI BlnI
BlpI Bpu1102I BsiWI BspLU11I BsrBI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII CpoI DraIII DrdI
Eco47III Eco81I EcoNI EcoRI EcoRV
FbaI FseI FspI HpaI KasI
KpnI KspI MluI NarI NheI
NotI NruI NspV PaeR7I PinAI
PmeI PshAI PvuI RsrII SacII
SbfI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1935 GACGTC GACGT^C
AclI 4051 AACGTT AA^CGTT
AflII 545 CTTAAG C^TTAAG
Asp700I 2297 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 275 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1316 TGGCCA TGG^CCA
BbrPI 1109 CACGTG CAC^GTG
BbvCI 79 CCTCAGC CCTCAGC(-5/-2)
BfrI 545 CTTAAG C^TTAAG
BglI 728 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1109 CACGTG [CT]AC^GT[GA]
BsaBI 1238 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1937 CGTCTC CGTCTC(1/5)
BspDI 3250 ATCGAT AT^CGAT
BssSI 1571 CACGAG CACGAG(-5/-1)
BstEII 3432 GGTGACC G^GT[ACGT]ACC
BstPI 3432 GGTGACC G^GT[ACGT]ACC
BstXI 750 CCATGATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 3250 ATCGAT AT^CGAT
EcoO65I 3432 GGTGACC G^GT[ACGT]ACC
HaeII 43 GGCGCT [GA]GCGC^[CT]
HindIII 474 AAGCTT A^AGCTT
MamI 1238 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1316 TGGCCA TGG^CCA
MscI 1316 TGGCCA TGG^CCA
NcoI 2801 CCATGG C^CATGG
NdeI 3847 CATATG CA^TATG
PacI 3702 TTAATTAA TTAAT^TAA
PflFI 275 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1109 CACGTG CAC^GTG
PmlI 1109 CACGTG CAC^GTG
Psp1406I 4051 AACGTT AA^CGTT
PstI 1116 CTGCAG CTGCA^G
SacI 3196 GAGCTC GAGCT^C
SapI 2027 GCTCTTC GCTCTTC(1/4)
SmaI 1363 CCCGGG CCC^GGG
SpeI 2068 ACTAGT A^CTAGT
SwaI 2498 ATTTAAAT ATTT^AAAT
Tth111I 275 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1554 CCACCTGCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1363 CCCGGG C^CCGGG
XmaI 1363 CCCGGG C^CCGGG
XmnI 2297 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 17 GTCGAC GT^[AC][GT]AC
2167 GTCGAC
AccIII 1581 TCCGGA T^CCGGA
1925 TCCGGA
AcyI 1935 GACGTC G[GA]^CG[CT]C
3091 GGCGTC
AflIII 2299 ACGTGT A^C[GA][CT]GT
3961 ACGTGT
BamHI 1578 GGATCC G^GATCC
3970 GGATCC
BanI 37 GGTGCC G^G[CT][GA]CC
996 GGTGCC
BciVI 450 GTATCC GTATCC(6/5)
1789 GTATCC
BglII 531 AGATCT A^GATCT
1798 AGATCT
BsaHI 1935 GACGTC G[GA]^CG[CT]C
3091 GGCGTC
BsaI 3458 GAGACC GGTCTC(1/5)
2158 GGTCTC
BseAI 1581 TCCGGA T^CCGGA
1925 TCCGGA
BsiEI 144 CGGCCG CG[GA][CT]^CG
157 CGGCCG
BspEI 1581 TCCGGA T^CCGGA
1925 TCCGGA
BsrDI 1881 CATTGC GCAATG(2/0)
1250 GCAATG
BsrFI 40 GCCGGC [GA]^CCGG[CT]
177 GCCGGC
Cfr10I 40 GCCGGC [GA]^CCGG[CT]
177 GCCGGC
DsaI 1942 CCACGG C^C[GA][CT]GG
2801 CCATGG
EagI 144 CGGCCG C^GGCCG
157 CGGCCG
EclXI 144 CGGCCG C^GGCCG
157 CGGCCG
Eco52I 144 CGGCCG C^GGCCG
157 CGGCCG
Hin1I 1935 GACGTC G[GA]^CG[CT]C
3091 GGCGTC
MroI 1581 TCCGGA T^CCGGA
1925 TCCGGA
NaeI 40 GCCGGC GCC^GGC
177 GCCGGC
NgoMIV 40 GCCGGC G^CCGGC
177 GCCGGC
PflMI 778 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3437 CCAAGGTTTGG
SalI 17 GTCGAC G^TCGAC
2167 GTCGAC
ScaI 612 AGTACT AGT^ACT
1092 AGTACT
SexAI 1263 ACCAGGT A^CC[AT]GGT
1389 ACCTGGT
SfcI 262 CTACAG C^T[GA][CT]AG
1116 CTGCAG
SphI 3371 GCATGC GCATG^C
3740 GCATGC
SspI 1829 AATATT AAT^ATT
4082 AATATT
StuI 549 AGGCCT AGG^CCT
2945 AGGCCT
Van91I 778 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3437 CCAAGGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AhdI
AluI AlwI AlwNI ApoI AseI
AsnI AspEI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI Eam1105I EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MspI MunI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PshBI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII